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Differentiation of myeloid leukemic cells in vitro demonstrated by microcalorimetry : Stimulation of leukemic and remission cells by IgG-binding Fc receptors

Fäldt, R. LU and Ankerst, J. LU orcid (1986) In Leukemia Research 10(9). p.1147-1150
Abstract

Interaction of immunoglobulin G (IgG)-coated latex particles with Fc receptors on myeloid leukemic blood cells and on polymorphonuclear granulocytes (PMN) from remission patients and healthy blood donors was investigated using microcalorimetry. The induced heat production by leukemic cells from 13 patients with the M2, M4 and M5 FAB groups (French-American-British classification) of acute myeloid leukemia (AML) was significantly higher than that of leukemic cells from 7 patients with the M1 FAB group (p < 0.005) and mononuclear blood cells from 10 healthy individuals (p < 0.005). The values were similar for PMN from 10 remission patients and 10 healthy blood donors. After incubation of M1 cells in vitro for 24-30 h at 37°C the... (More)

Interaction of immunoglobulin G (IgG)-coated latex particles with Fc receptors on myeloid leukemic blood cells and on polymorphonuclear granulocytes (PMN) from remission patients and healthy blood donors was investigated using microcalorimetry. The induced heat production by leukemic cells from 13 patients with the M2, M4 and M5 FAB groups (French-American-British classification) of acute myeloid leukemia (AML) was significantly higher than that of leukemic cells from 7 patients with the M1 FAB group (p < 0.005) and mononuclear blood cells from 10 healthy individuals (p < 0.005). The values were similar for PMN from 10 remission patients and 10 healthy blood donors. After incubation of M1 cells in vitro for 24-30 h at 37°C the heat production induced by IgG-coated latex particles by the cells increased significantly, indicating the appearance of Fc receptors for IgG. In addition, the heat production by unstimulated M2, M4 and M5 cells was significantly higher than that by unstimulated M1 cells (p < 0.005) and normal mononuclear cells (p < 0.0005). The heat production by unstimulated PMN suspended in tissue culture medium was similar in remission patients and healthy blood donors.

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author
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organization
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type
Contribution to journal
publication status
published
subject
keywords
Acute myeloid leukemia, cellular differentiation, Fc receptors, microcalorimetry
in
Leukemia Research
volume
10
issue
9
pages
1147 - 1150
publisher
Elsevier
external identifiers
  • scopus:0022530564
  • pmid:2945056
ISSN
0145-2126
DOI
10.1016/0145-2126(86)90060-3
language
English
LU publication?
yes
id
c30489a1-bc4f-4573-9d73-885aa0d2d40d
date added to LUP
2021-01-15 15:20:33
date last changed
2024-01-03 04:42:35
@article{c30489a1-bc4f-4573-9d73-885aa0d2d40d,
  abstract     = {{<p>Interaction of immunoglobulin G (IgG)-coated latex particles with Fc receptors on myeloid leukemic blood cells and on polymorphonuclear granulocytes (PMN) from remission patients and healthy blood donors was investigated using microcalorimetry. The induced heat production by leukemic cells from 13 patients with the M2, M4 and M5 FAB groups (French-American-British classification) of acute myeloid leukemia (AML) was significantly higher than that of leukemic cells from 7 patients with the M1 FAB group (p &lt; 0.005) and mononuclear blood cells from 10 healthy individuals (p &lt; 0.005). The values were similar for PMN from 10 remission patients and 10 healthy blood donors. After incubation of M1 cells in vitro for 24-30 h at 37°C the heat production induced by IgG-coated latex particles by the cells increased significantly, indicating the appearance of Fc receptors for IgG. In addition, the heat production by unstimulated M2, M4 and M5 cells was significantly higher than that by unstimulated M1 cells (p &lt; 0.005) and normal mononuclear cells (p &lt; 0.0005). The heat production by unstimulated PMN suspended in tissue culture medium was similar in remission patients and healthy blood donors.</p>}},
  author       = {{Fäldt, R. and Ankerst, J.}},
  issn         = {{0145-2126}},
  keywords     = {{Acute myeloid leukemia; cellular differentiation; Fc receptors; microcalorimetry}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{1147--1150}},
  publisher    = {{Elsevier}},
  series       = {{Leukemia Research}},
  title        = {{Differentiation of myeloid leukemic cells in vitro demonstrated by microcalorimetry : Stimulation of leukemic and remission cells by IgG-binding Fc receptors}},
  url          = {{http://dx.doi.org/10.1016/0145-2126(86)90060-3}},
  doi          = {{10.1016/0145-2126(86)90060-3}},
  volume       = {{10}},
  year         = {{1986}},
}