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Microcontact-BSA imprinted capacitive biosensor for real-time, sensitive and selective detection of BSA

Ertürk, Gizem LU ; Berillo, Dmitriy LU ; Hedström, Martin LU and Mattiasson, Bo LU (2014) In Biotechnology Reports 3. p.65-72
Abstract

An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 × 10-20-1.0 × 10-8 M with a limit of detection (LOD) of 1.0 × 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for >70 assays during 2... (More)

An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 × 10-20-1.0 × 10-8 M with a limit of detection (LOD) of 1.0 × 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for >70 assays during 2 months and retained their binding properties during all that time. The NIP (non-imprinted) electrode was used as a reference. The microcontact imprinting technology combined with the biosensor applications is a promising technology for future applications.

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publication status
published
subject
keywords
Journal Article
in
Biotechnology Reports
volume
3
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:84904895683
  • pmid:28626651
ISSN
2215-017X
DOI
10.1016/j.btre.2014.06.006
language
English
LU publication?
yes
id
c3371d52-4a86-4e71-a591-24193dbc1a67
date added to LUP
2018-01-25 13:44:04
date last changed
2021-10-06 05:12:25
@article{c3371d52-4a86-4e71-a591-24193dbc1a67,
  abstract     = {<p>An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 × 10-20-1.0 × 10-8 M with a limit of detection (LOD) of 1.0 × 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for &gt;70 assays during 2 months and retained their binding properties during all that time. The NIP (non-imprinted) electrode was used as a reference. The microcontact imprinting technology combined with the biosensor applications is a promising technology for future applications.</p>},
  author       = {Ertürk, Gizem and Berillo, Dmitriy and Hedström, Martin and Mattiasson, Bo},
  issn         = {2215-017X},
  language     = {eng},
  pages        = {65--72},
  publisher    = {Elsevier},
  series       = {Biotechnology Reports},
  title        = {Microcontact-BSA imprinted capacitive biosensor for real-time, sensitive and selective detection of BSA},
  url          = {http://dx.doi.org/10.1016/j.btre.2014.06.006},
  doi          = {10.1016/j.btre.2014.06.006},
  volume       = {3},
  year         = {2014},
}