Microcontact-BSA imprinted capacitive biosensor for real-time, sensitive and selective detection of BSA
(2014) In Biotechnology Reports 3. p.65-72- Abstract
An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 × 10-20-1.0 × 10-8 M with a limit of detection (LOD) of 1.0 × 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for >70 assays during 2... (More)
An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 × 10-20-1.0 × 10-8 M with a limit of detection (LOD) of 1.0 × 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for >70 assays during 2 months and retained their binding properties during all that time. The NIP (non-imprinted) electrode was used as a reference. The microcontact imprinting technology combined with the biosensor applications is a promising technology for future applications.
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- author
- Ertürk, Gizem LU ; Berillo, Dmitriy LU ; Hedström, Martin LU and Mattiasson, Bo LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Journal Article
- in
- Biotechnology Reports
- volume
- 3
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:28626651
- scopus:84904895683
- ISSN
- 2215-017X
- DOI
- 10.1016/j.btre.2014.06.006
- language
- English
- LU publication?
- yes
- id
- c3371d52-4a86-4e71-a591-24193dbc1a67
- date added to LUP
- 2018-01-25 13:44:04
- date last changed
- 2024-06-10 07:08:28
@article{c3371d52-4a86-4e71-a591-24193dbc1a67,
abstract = {{<p>An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 × 10-20-1.0 × 10-8 M with a limit of detection (LOD) of 1.0 × 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for >70 assays during 2 months and retained their binding properties during all that time. The NIP (non-imprinted) electrode was used as a reference. The microcontact imprinting technology combined with the biosensor applications is a promising technology for future applications.</p>}},
author = {{Ertürk, Gizem and Berillo, Dmitriy and Hedström, Martin and Mattiasson, Bo}},
issn = {{2215-017X}},
keywords = {{Journal Article}},
language = {{eng}},
pages = {{65--72}},
publisher = {{Elsevier}},
series = {{Biotechnology Reports}},
title = {{Microcontact-BSA imprinted capacitive biosensor for real-time, sensitive and selective detection of BSA}},
url = {{http://dx.doi.org/10.1016/j.btre.2014.06.006}},
doi = {{10.1016/j.btre.2014.06.006}},
volume = {{3}},
year = {{2014}},
}