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Growth cones of regenerating adult sciatic sensory axons release axonally transported proteins

Remgård, Pär; Edbladh, Magnus LU ; Ekström, Per A R LU and Edström, Anders LU (1992) In Brain Research 572(1-2). p.139-145
Abstract

Labelled, rapidly transported axonal proteins were shown to be released frog adult frog sciatic sensory neurons, regenerating in vitro after a crush injury. The spatial distribution of the transported, released proteins could accurately be resolved by culturing the nerve on nitrocellulose paper, which trapped the released proteins. The release was located to the crush and to the entire outgrowth region. When regeneration was inhibited by adenosine, the release was limited to the crush site, implying that the release was linked to the growing axons. Other experiments suggested that the release emanated from growth cones. Furthermore, two-dimensional electrophoretical analysis of both fast axonally transported and of released proteins... (More)

Labelled, rapidly transported axonal proteins were shown to be released frog adult frog sciatic sensory neurons, regenerating in vitro after a crush injury. The spatial distribution of the transported, released proteins could accurately be resolved by culturing the nerve on nitrocellulose paper, which trapped the released proteins. The release was located to the crush and to the entire outgrowth region. When regeneration was inhibited by adenosine, the release was limited to the crush site, implying that the release was linked to the growing axons. Other experiments suggested that the release emanated from growth cones. Furthermore, two-dimensional electrophoretical analysis of both fast axonally transported and of released proteins showed that the represented a selection of the transported protein species. © 1992.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Axonal transport, Frog, Growth cone, Regeneration, Release, Sciatic nerve
in
Brain Research
volume
572
issue
1-2
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:0026505364
ISSN
0006-8993
DOI
10.1016/0006-8993(92)90462-I
language
English
LU publication?
yes
id
c33d238b-4980-4976-9804-3f8d09561d98
date added to LUP
2016-12-07 14:37:52
date last changed
2017-01-27 15:29:56
@article{c33d238b-4980-4976-9804-3f8d09561d98,
  abstract     = {<p>Labelled, rapidly transported axonal proteins were shown to be released frog adult frog sciatic sensory neurons, regenerating in vitro after a crush injury. The spatial distribution of the transported, released proteins could accurately be resolved by culturing the nerve on nitrocellulose paper, which trapped the released proteins. The release was located to the crush and to the entire outgrowth region. When regeneration was inhibited by adenosine, the release was limited to the crush site, implying that the release was linked to the growing axons. Other experiments suggested that the release emanated from growth cones. Furthermore, two-dimensional electrophoretical analysis of both fast axonally transported and of released proteins showed that the represented a selection of the transported protein species. © 1992.</p>},
  author       = {Remgård, Pär and Edbladh, Magnus and Ekström, Per A R and Edström, Anders},
  issn         = {0006-8993},
  keyword      = {Axonal transport,Frog,Growth cone,Regeneration,Release,Sciatic nerve},
  language     = {eng},
  month        = {02},
  number       = {1-2},
  pages        = {139--145},
  publisher    = {Elsevier},
  series       = {Brain Research},
  title        = {Growth cones of regenerating adult sciatic sensory axons release axonally transported proteins},
  url          = {http://dx.doi.org/10.1016/0006-8993(92)90462-I},
  volume       = {572},
  year         = {1992},
}