Cobalt nanoparticles cause allergic contact dermatitis in humans
(2023) In British Journal of Dermatology 188(2). p.278-287- Abstract
Background: Cobalt (Co) causes allergic contact dermatitis (ACD) and the emerging use of Co nanoparticles (CoNPs) warrants gaining further insight into its potential to elicit ACD in sensitized individuals. Objectives: The aims of the study were to clarify to what extent CoNPs may elicit ACD responses in participants with Co contact allergy, and to evaluate whether the nanoparticles cause a distinct immune response compared with cobalt chloride (CoCl2) in the skin reactions. Methods: Fourteen individuals with Co contact allergy were exposed to CoNPs, CoCl2, a Co-containing hard-metal disc (positive control), and an empty test chamber (negative control) by patch testing. Allergic responses were evaluated clinically by a dermatologist at... (More)
Background: Cobalt (Co) causes allergic contact dermatitis (ACD) and the emerging use of Co nanoparticles (CoNPs) warrants gaining further insight into its potential to elicit ACD in sensitized individuals. Objectives: The aims of the study were to clarify to what extent CoNPs may elicit ACD responses in participants with Co contact allergy, and to evaluate whether the nanoparticles cause a distinct immune response compared with cobalt chloride (CoCl2) in the skin reactions. Methods: Fourteen individuals with Co contact allergy were exposed to CoNPs, CoCl2, a Co-containing hard-metal disc (positive control), and an empty test chamber (negative control) by patch testing. Allergic responses were evaluated clinically by a dermatologist at Days 2, 4 and 7. At Day 2, patch-test chambers were removed, and remaining test-substance and skin-wipe samples were collected for inductive-coupled plasma mass spectrometry (ICP-MS) analysis. Additionally, skin biopsies were taken from patch-test reactions at Day 4 for quantitative real-time polymerase chain reaction analysis, histopathology and ICP-MS analysis of Co skin penetration. Results: Patch testing with CoNPs elicited allergic reactions in Co-sensitized individuals. At all timepoints, clinical assessment revealed significantly lower frequencies of positive patch-test reactions to CoNPs compared with CoCl2 or to the positive control. CoNPs elicited comparable immune responses to CoCl2. Chemical analysis of Co residues in patch-test filters, and on skin, shows lower doses for CoNPs compared with CoCl2. Conclusions: CoNPs potently elicit immune responses in Co-sensitized individuals. Even though patch testing with CoNPs resulted in a lower skin dose than CoCl2, identical immunological profiles were present. Further research is needed to identify the potential harm of CoNPs to human health.
(Less)
- author
- Midander, Klara ; Werner, Paulina ; Isaksson, Marlene LU ; Wisgrill, Lukas ; Liden, Carola ; Fyhrquist, Nanna and Julander, Anneli
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- in
- British Journal of Dermatology
- volume
- 188
- issue
- 2
- pages
- 10 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:36637098
- scopus:85153965959
- ISSN
- 0007-0963
- DOI
- 10.1093/bjd/ljac043
- language
- English
- LU publication?
- yes
- id
- c3cedfc4-1f14-4d16-a323-90fe380d74bc
- date added to LUP
- 2023-07-14 09:19:16
- date last changed
- 2024-04-19 23:25:34
@article{c3cedfc4-1f14-4d16-a323-90fe380d74bc,
abstract = {{<p>Background: Cobalt (Co) causes allergic contact dermatitis (ACD) and the emerging use of Co nanoparticles (CoNPs) warrants gaining further insight into its potential to elicit ACD in sensitized individuals. Objectives: The aims of the study were to clarify to what extent CoNPs may elicit ACD responses in participants with Co contact allergy, and to evaluate whether the nanoparticles cause a distinct immune response compared with cobalt chloride (CoCl2) in the skin reactions. Methods: Fourteen individuals with Co contact allergy were exposed to CoNPs, CoCl2, a Co-containing hard-metal disc (positive control), and an empty test chamber (negative control) by patch testing. Allergic responses were evaluated clinically by a dermatologist at Days 2, 4 and 7. At Day 2, patch-test chambers were removed, and remaining test-substance and skin-wipe samples were collected for inductive-coupled plasma mass spectrometry (ICP-MS) analysis. Additionally, skin biopsies were taken from patch-test reactions at Day 4 for quantitative real-time polymerase chain reaction analysis, histopathology and ICP-MS analysis of Co skin penetration. Results: Patch testing with CoNPs elicited allergic reactions in Co-sensitized individuals. At all timepoints, clinical assessment revealed significantly lower frequencies of positive patch-test reactions to CoNPs compared with CoCl2 or to the positive control. CoNPs elicited comparable immune responses to CoCl2. Chemical analysis of Co residues in patch-test filters, and on skin, shows lower doses for CoNPs compared with CoCl2. Conclusions: CoNPs potently elicit immune responses in Co-sensitized individuals. Even though patch testing with CoNPs resulted in a lower skin dose than CoCl2, identical immunological profiles were present. Further research is needed to identify the potential harm of CoNPs to human health.</p>}},
author = {{Midander, Klara and Werner, Paulina and Isaksson, Marlene and Wisgrill, Lukas and Liden, Carola and Fyhrquist, Nanna and Julander, Anneli}},
issn = {{0007-0963}},
language = {{eng}},
number = {{2}},
pages = {{278--287}},
publisher = {{Wiley-Blackwell}},
series = {{British Journal of Dermatology}},
title = {{Cobalt nanoparticles cause allergic contact dermatitis in humans}},
url = {{http://dx.doi.org/10.1093/bjd/ljac043}},
doi = {{10.1093/bjd/ljac043}},
volume = {{188}},
year = {{2023}},
}