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Water Sorption and Glass Transition of Pig Gastric Mucin Studied by QCM-D.

Znamenskaya, Yana ; Sotres, Javier ; Gavryushov, Sergei LU ; Engblom, Johan ; Arnebrant, Thomas and Kocherbitov, Vitaly LU (2013) In The Journal of Physical Chemistry Part B 117(8). p.2554-2563
Abstract
Hydration of films of pig gastric mucin was studied using a quartz crystal microbalance with dissipation monitoring (QCM-D) equipped with a humidity module. As a prerequisite, the water adsorption isotherm of a clean silica surface was determined. Atomic force microscopy was used to characterize the changes occurring on the silica surface after repeated sorption/desorption and cleaning cycles. The water sorption isotherms of several hundreds of nanometers thick mucin films were obtained in QCM-D experiments using analysis of overtone behavior. The results show that the sorption isotherms are not dependent on the film thicknesses and are in good agreement with sorption calorimetric data on mucin in the bulk phase. Moreover,... (More)
Hydration of films of pig gastric mucin was studied using a quartz crystal microbalance with dissipation monitoring (QCM-D) equipped with a humidity module. As a prerequisite, the water adsorption isotherm of a clean silica surface was determined. Atomic force microscopy was used to characterize the changes occurring on the silica surface after repeated sorption/desorption and cleaning cycles. The water sorption isotherms of several hundreds of nanometers thick mucin films were obtained in QCM-D experiments using analysis of overtone behavior. The results show that the sorption isotherms are not dependent on the film thicknesses and are in good agreement with sorption calorimetric data on mucin in the bulk phase. Moreover, hydration-induced changes of rheological properties of mucin films were investigated using a model-free approach. The ratio of G'/G″ was evaluated as a function of relative humidity. The transition from solidlike behavior to liquidlike behavior was observed in the same humidity range as in sorption calorimetric experiments. Thus, ability of QCM-D to monitor glass transition in biopolymers was demonstrated. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of Physical Chemistry Part B
volume
117
issue
8
pages
2554 - 2563
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:23369097
  • wos:000315707900037
  • scopus:84874604458
  • pmid:23369097
ISSN
1520-5207
DOI
10.1021/jp311968b
language
English
LU publication?
yes
id
c48c7ade-3376-4524-b213-46096cfaa3d8 (old id 3560353)
date added to LUP
2016-04-01 14:43:08
date last changed
2022-02-19 20:30:33
@article{c48c7ade-3376-4524-b213-46096cfaa3d8,
  abstract     = {{Hydration of films of pig gastric mucin was studied using a quartz crystal microbalance with dissipation monitoring (QCM-D) equipped with a humidity module. As a prerequisite, the water adsorption isotherm of a clean silica surface was determined. Atomic force microscopy was used to characterize the changes occurring on the silica surface after repeated sorption/desorption and cleaning cycles. The water sorption isotherms of several hundreds of nanometers thick mucin films were obtained in QCM-D experiments using analysis of overtone behavior. The results show that the sorption isotherms are not dependent on the film thicknesses and are in good agreement with sorption calorimetric data on mucin in the bulk phase. Moreover, hydration-induced changes of rheological properties of mucin films were investigated using a model-free approach. The ratio of G'/G″ was evaluated as a function of relative humidity. The transition from solidlike behavior to liquidlike behavior was observed in the same humidity range as in sorption calorimetric experiments. Thus, ability of QCM-D to monitor glass transition in biopolymers was demonstrated.}},
  author       = {{Znamenskaya, Yana and Sotres, Javier and Gavryushov, Sergei and Engblom, Johan and Arnebrant, Thomas and Kocherbitov, Vitaly}},
  issn         = {{1520-5207}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{2554--2563}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{The Journal of Physical Chemistry Part B}},
  title        = {{Water Sorption and Glass Transition of Pig Gastric Mucin Studied by QCM-D.}},
  url          = {{http://dx.doi.org/10.1021/jp311968b}},
  doi          = {{10.1021/jp311968b}},
  volume       = {{117}},
  year         = {{2013}},
}