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Impact of molecular linker size on physicochemical properties of assembled gold nanoparticle mono-/multi-layers and their applicability for functional binding of biomolecules

Krikstolaityte, Vida ; Hamit-Eminovski, Jildiz ; Abariute, Laura LU ; Niaura, Gediminas ; Meskys, Rolandas ; Arnebrant, Thomas ; Lisak, Grzegorz and Ruzgas, Tautgirdas LU (2019) In Journal of Colloid and Interface Science 543. p.307-316
Abstract

In this work the impact of molecular inter-linker size on gold nanoparticle (AuNP) mono-/multilayer structural properties, density and homogeneity has been investigated. These characteristics are of great importance for functional binding of biomolecules. Positively charged high or low molecular weight inter-linkers, poly-L-lysine (PLL) or N-(6-mercapto)hexylpyridinium (MHP), were used to attach negatively charged AuNPs on a planar gold surface as well as to further interlink into a multilayer structure via layer-by-layer deposition. The inter-particle interaction within the assembled AuNP films was adjusted by the ionic strength in the AuNPs dispersions. The AuNP layer density and structural/viscoelastic properties were evaluated by... (More)

In this work the impact of molecular inter-linker size on gold nanoparticle (AuNP) mono-/multilayer structural properties, density and homogeneity has been investigated. These characteristics are of great importance for functional binding of biomolecules. Positively charged high or low molecular weight inter-linkers, poly-L-lysine (PLL) or N-(6-mercapto)hexylpyridinium (MHP), were used to attach negatively charged AuNPs on a planar gold surface as well as to further interlink into a multilayer structure via layer-by-layer deposition. The inter-particle interaction within the assembled AuNP films was adjusted by the ionic strength in the AuNPs dispersions. The AuNP layer density and structural/viscoelastic properties were evaluated by the quartz crystal microbalance with dissipation (QCM-D) technique. The validity of the commercial Voigt model, specifically developed for quantitative QCM-D data analysis of homogeneous viscoelastic films, was evaluated by a model independent analysis when comparing the assembled AuNP films with a homogeneous layer of a mucin from bovine submaxillary glands. Both AuNP mono- and multilayers, attached/interlinked via long flexible PLL molecules assembled to denser and more soft/viscous structures compared to those interlinked by short MHP compounds. Thus, PLL-interlinked AuNP mono-/multilayer structures were further investigated as a platform for laccase enzyme functional adsorption via qualitative assessment of bioelectrochemical characteristics of the enzyme.

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organization
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publication status
published
subject
keywords
Bio-electro-catalysis, Biomolecule adsorption, Electrochemical-quartz crystal microbalance with dissipation, Gold nanoparticles, Layer-by-layer deposition, N-(6-mercaptohexyl)pyridinium, Poly-L-lysine
in
Journal of Colloid and Interface Science
volume
543
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:85062011660
  • pmid:30825679
ISSN
0021-9797
DOI
10.1016/j.jcis.2019.02.048
language
English
LU publication?
yes
id
c4d67419-c547-4917-8f4e-50af69ec1e82
date added to LUP
2019-03-05 14:17:56
date last changed
2020-02-19 05:21:48
@article{c4d67419-c547-4917-8f4e-50af69ec1e82,
  abstract     = {<p>In this work the impact of molecular inter-linker size on gold nanoparticle (AuNP) mono-/multilayer structural properties, density and homogeneity has been investigated. These characteristics are of great importance for functional binding of biomolecules. Positively charged high or low molecular weight inter-linkers, poly-L-lysine (PLL) or N-(6-mercapto)hexylpyridinium (MHP), were used to attach negatively charged AuNPs on a planar gold surface as well as to further interlink into a multilayer structure via layer-by-layer deposition. The inter-particle interaction within the assembled AuNP films was adjusted by the ionic strength in the AuNPs dispersions. The AuNP layer density and structural/viscoelastic properties were evaluated by the quartz crystal microbalance with dissipation (QCM-D) technique. The validity of the commercial Voigt model, specifically developed for quantitative QCM-D data analysis of homogeneous viscoelastic films, was evaluated by a model independent analysis when comparing the assembled AuNP films with a homogeneous layer of a mucin from bovine submaxillary glands. Both AuNP mono- and multilayers, attached/interlinked via long flexible PLL molecules assembled to denser and more soft/viscous structures compared to those interlinked by short MHP compounds. Thus, PLL-interlinked AuNP mono-/multilayer structures were further investigated as a platform for laccase enzyme functional adsorption via qualitative assessment of bioelectrochemical characteristics of the enzyme.</p>},
  author       = {Krikstolaityte, Vida and Hamit-Eminovski, Jildiz and Abariute, Laura and Niaura, Gediminas and Meskys, Rolandas and Arnebrant, Thomas and Lisak, Grzegorz and Ruzgas, Tautgirdas},
  issn         = {0021-9797},
  language     = {eng},
  pages        = {307--316},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {Impact of molecular linker size on physicochemical properties of assembled gold nanoparticle mono-/multi-layers and their applicability for functional binding of biomolecules},
  url          = {http://dx.doi.org/10.1016/j.jcis.2019.02.048},
  doi          = {10.1016/j.jcis.2019.02.048},
  volume       = {543},
  year         = {2019},
}