Impact of molecular linker size on physicochemical properties of assembled gold nanoparticle mono-/multi-layers and their applicability for functional binding of biomolecules
(2019) In Journal of Colloid and Interface Science 543. p.307-316- Abstract
In this work the impact of molecular inter-linker size on gold nanoparticle (AuNP) mono-/multilayer structural properties, density and homogeneity has been investigated. These characteristics are of great importance for functional binding of biomolecules. Positively charged high or low molecular weight inter-linkers, poly-L-lysine (PLL) or N-(6-mercapto)hexylpyridinium (MHP), were used to attach negatively charged AuNPs on a planar gold surface as well as to further interlink into a multilayer structure via layer-by-layer deposition. The inter-particle interaction within the assembled AuNP films was adjusted by the ionic strength in the AuNPs dispersions. The AuNP layer density and structural/viscoelastic properties were evaluated by... (More)
In this work the impact of molecular inter-linker size on gold nanoparticle (AuNP) mono-/multilayer structural properties, density and homogeneity has been investigated. These characteristics are of great importance for functional binding of biomolecules. Positively charged high or low molecular weight inter-linkers, poly-L-lysine (PLL) or N-(6-mercapto)hexylpyridinium (MHP), were used to attach negatively charged AuNPs on a planar gold surface as well as to further interlink into a multilayer structure via layer-by-layer deposition. The inter-particle interaction within the assembled AuNP films was adjusted by the ionic strength in the AuNPs dispersions. The AuNP layer density and structural/viscoelastic properties were evaluated by the quartz crystal microbalance with dissipation (QCM-D) technique. The validity of the commercial Voigt model, specifically developed for quantitative QCM-D data analysis of homogeneous viscoelastic films, was evaluated by a model independent analysis when comparing the assembled AuNP films with a homogeneous layer of a mucin from bovine submaxillary glands. Both AuNP mono- and multilayers, attached/interlinked via long flexible PLL molecules assembled to denser and more soft/viscous structures compared to those interlinked by short MHP compounds. Thus, PLL-interlinked AuNP mono-/multilayer structures were further investigated as a platform for laccase enzyme functional adsorption via qualitative assessment of bioelectrochemical characteristics of the enzyme.
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- author
- Krikstolaityte, Vida ; Hamit-Eminovski, Jildiz ; Abariute, Laura LU ; Niaura, Gediminas ; Meskys, Rolandas ; Arnebrant, Thomas ; Lisak, Grzegorz and Ruzgas, Tautgirdas LU
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bio-electro-catalysis, Biomolecule adsorption, Electrochemical-quartz crystal microbalance with dissipation, Gold nanoparticles, Layer-by-layer deposition, N-(6-mercaptohexyl)pyridinium, Poly-L-lysine
- in
- Journal of Colloid and Interface Science
- volume
- 543
- pages
- 10 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85062011660
- pmid:30825679
- ISSN
- 0021-9797
- DOI
- 10.1016/j.jcis.2019.02.048
- language
- English
- LU publication?
- yes
- id
- c4d67419-c547-4917-8f4e-50af69ec1e82
- date added to LUP
- 2019-03-05 14:17:56
- date last changed
- 2024-06-12 08:39:00
@article{c4d67419-c547-4917-8f4e-50af69ec1e82,
abstract = {{<p>In this work the impact of molecular inter-linker size on gold nanoparticle (AuNP) mono-/multilayer structural properties, density and homogeneity has been investigated. These characteristics are of great importance for functional binding of biomolecules. Positively charged high or low molecular weight inter-linkers, poly-L-lysine (PLL) or N-(6-mercapto)hexylpyridinium (MHP), were used to attach negatively charged AuNPs on a planar gold surface as well as to further interlink into a multilayer structure via layer-by-layer deposition. The inter-particle interaction within the assembled AuNP films was adjusted by the ionic strength in the AuNPs dispersions. The AuNP layer density and structural/viscoelastic properties were evaluated by the quartz crystal microbalance with dissipation (QCM-D) technique. The validity of the commercial Voigt model, specifically developed for quantitative QCM-D data analysis of homogeneous viscoelastic films, was evaluated by a model independent analysis when comparing the assembled AuNP films with a homogeneous layer of a mucin from bovine submaxillary glands. Both AuNP mono- and multilayers, attached/interlinked via long flexible PLL molecules assembled to denser and more soft/viscous structures compared to those interlinked by short MHP compounds. Thus, PLL-interlinked AuNP mono-/multilayer structures were further investigated as a platform for laccase enzyme functional adsorption via qualitative assessment of bioelectrochemical characteristics of the enzyme.</p>}},
author = {{Krikstolaityte, Vida and Hamit-Eminovski, Jildiz and Abariute, Laura and Niaura, Gediminas and Meskys, Rolandas and Arnebrant, Thomas and Lisak, Grzegorz and Ruzgas, Tautgirdas}},
issn = {{0021-9797}},
keywords = {{Bio-electro-catalysis; Biomolecule adsorption; Electrochemical-quartz crystal microbalance with dissipation; Gold nanoparticles; Layer-by-layer deposition; N-(6-mercaptohexyl)pyridinium; Poly-L-lysine}},
language = {{eng}},
pages = {{307--316}},
publisher = {{Elsevier}},
series = {{Journal of Colloid and Interface Science}},
title = {{Impact of molecular linker size on physicochemical properties of assembled gold nanoparticle mono-/multi-layers and their applicability for functional binding of biomolecules}},
url = {{http://dx.doi.org/10.1016/j.jcis.2019.02.048}},
doi = {{10.1016/j.jcis.2019.02.048}},
volume = {{543}},
year = {{2019}},
}