Stable lodinated polypeptide hormones prepared by polyacrylamide gel electrophoresis
(1980) In Analytical Biochemistry 107(1). p.165-176- Abstract
Iodination of several insulin and proinsulin preparations, human growth hormone and bovine pancreatic polypeptide was performed using H2O2 and lactoperoxidase or chloramine T. The iodination mixtures were fractionated by polyacrylamide gel electrophoresis at pH 9.15 in long gel rods followed by simple elution of the iodinated products from thin gel slices. With this method 125I tracers with long shelf life and high specific activity suitable for radioimmunoassays could easily be obtained.
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- author
- Linde, Susanne ; Hansen, Bruno and Lernmark, Åke LU
- publishing date
- 1980-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Biochemistry
- volume
- 107
- issue
- 1
- pages
- 12 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:7001948
- scopus:0019167449
- ISSN
- 0003-2697
- DOI
- 10.1016/0003-2697(80)90507-2
- language
- English
- LU publication?
- no
- id
- c5097e5f-793f-4677-935e-87d792905e1b
- date added to LUP
- 2019-12-12 23:25:27
- date last changed
- 2024-03-13 08:26:20
@article{c5097e5f-793f-4677-935e-87d792905e1b, abstract = {{<p>Iodination of several insulin and proinsulin preparations, human growth hormone and bovine pancreatic polypeptide was performed using H<sub>2</sub>O<sub>2</sub> and lactoperoxidase or chloramine T. The iodination mixtures were fractionated by polyacrylamide gel electrophoresis at pH 9.15 in long gel rods followed by simple elution of the iodinated products from thin gel slices. With this method <sup>125</sup>I tracers with long shelf life and high specific activity suitable for radioimmunoassays could easily be obtained.</p>}}, author = {{Linde, Susanne and Hansen, Bruno and Lernmark, Åke}}, issn = {{0003-2697}}, language = {{eng}}, month = {{01}}, number = {{1}}, pages = {{165--176}}, publisher = {{Elsevier}}, series = {{Analytical Biochemistry}}, title = {{Stable lodinated polypeptide hormones prepared by polyacrylamide gel electrophoresis}}, url = {{http://dx.doi.org/10.1016/0003-2697(80)90507-2}}, doi = {{10.1016/0003-2697(80)90507-2}}, volume = {{107}}, year = {{1980}}, }