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Stable lodinated polypeptide hormones prepared by polyacrylamide gel electrophoresis

Linde, Susanne ; Hansen, Bruno and Lernmark, Åke LU orcid (1980) In Analytical Biochemistry 107(1). p.165-176
Abstract

Iodination of several insulin and proinsulin preparations, human growth hormone and bovine pancreatic polypeptide was performed using H2O2 and lactoperoxidase or chloramine T. The iodination mixtures were fractionated by polyacrylamide gel electrophoresis at pH 9.15 in long gel rods followed by simple elution of the iodinated products from thin gel slices. With this method 125I tracers with long shelf life and high specific activity suitable for radioimmunoassays could easily be obtained.

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author
; and
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Biochemistry
volume
107
issue
1
pages
12 pages
publisher
Elsevier
external identifiers
  • pmid:7001948
  • scopus:0019167449
ISSN
0003-2697
DOI
10.1016/0003-2697(80)90507-2
language
English
LU publication?
no
id
c5097e5f-793f-4677-935e-87d792905e1b
date added to LUP
2019-12-12 23:25:27
date last changed
2024-03-13 08:26:20
@article{c5097e5f-793f-4677-935e-87d792905e1b,
  abstract     = {{<p>Iodination of several insulin and proinsulin preparations, human growth hormone and bovine pancreatic polypeptide was performed using H<sub>2</sub>O<sub>2</sub> and lactoperoxidase or chloramine T. The iodination mixtures were fractionated by polyacrylamide gel electrophoresis at pH 9.15 in long gel rods followed by simple elution of the iodinated products from thin gel slices. With this method <sup>125</sup>I tracers with long shelf life and high specific activity suitable for radioimmunoassays could easily be obtained.</p>}},
  author       = {{Linde, Susanne and Hansen, Bruno and Lernmark, Åke}},
  issn         = {{0003-2697}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1}},
  pages        = {{165--176}},
  publisher    = {{Elsevier}},
  series       = {{Analytical Biochemistry}},
  title        = {{Stable lodinated polypeptide hormones prepared by polyacrylamide gel electrophoresis}},
  url          = {{http://dx.doi.org/10.1016/0003-2697(80)90507-2}},
  doi          = {{10.1016/0003-2697(80)90507-2}},
  volume       = {{107}},
  year         = {{1980}},
}