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Production of moth sex pheromone precursors in Nicotiana spp. : a worthwhile new approach to pest control

Xia, Yi Han LU ; Ding, Bao Jian LU ; Wang, Hong Lei LU ; Hofvander, Per ; Jarl-Sunesson, Carin LU and Löfstedt, Christer LU (2020) In Journal of Pest Science 93(4). p.1333-1346
Abstract

Pheromones are environmentally friendly alternatives to traditional pesticides for pest control. They are widely applied for insect monitoring, mating disruption and mass trapping. Nicotiana benthamiana and N. tabacum are potential green biomass production platforms of moth sex pheromones. Using these two Nicotiana species as plant factories, we expressed biosynthetic genes of plant and insect origin in leaf tissue. Moth sex pheromone precursors (E)-11-tetradecenoic acid, (Z)-11-tetradecenoic acid and (Z)-11-hexadecenoic acid were produced by introducing the acyl-ACP thioesterases CpuFatB1 from Cuphea pulcherrima or CpaFatB2 from C. palustris and the fatty acyl desaturases Ave∆11 from Argyrotaenia velutinana, CpaE11 from Choristoneura... (More)

Pheromones are environmentally friendly alternatives to traditional pesticides for pest control. They are widely applied for insect monitoring, mating disruption and mass trapping. Nicotiana benthamiana and N. tabacum are potential green biomass production platforms of moth sex pheromones. Using these two Nicotiana species as plant factories, we expressed biosynthetic genes of plant and insect origin in leaf tissue. Moth sex pheromone precursors (E)-11-tetradecenoic acid, (Z)-11-tetradecenoic acid and (Z)-11-hexadecenoic acid were produced by introducing the acyl-ACP thioesterases CpuFatB1 from Cuphea pulcherrima or CpaFatB2 from C. palustris and the fatty acyl desaturases Ave∆11 from Argyrotaenia velutinana, CpaE11 from Choristoneura parallela or Atr∆11 from Amyelois transitella, under the control of CaMV-35S promoter. Among the Nicotiana spp. transformants, the best line produced (Z)-11-hexadecenoic acid at 17.6% of total fatty acids in leaves, during flowering stage, corresponding to 335 µg of (Z)-11-hexadecenoic acid per gram of fresh leaf. The (Z)-11-hexadecenoic acid production lines from N. benthamiana were selected for further propagation to obtain homozygous lines. In the N. benthamiana T2 generation, the production quantity of (Z)-11-hexadecenoic acid was stable. Our study demonstrates the feasibility of stable transformation of N. benthamiana for production of moth pheromone precursors in vegetative tissue.

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Contribution to journal
publication status
published
subject
keywords
Acyl-ACP thioesterase, Fatty acyl desaturase, Insect pheromone precursors, Nicotiana spp, Pest control, Stable transformation
in
Journal of Pest Science
volume
93
issue
4
pages
14 pages
publisher
Springer
external identifiers
  • scopus:85086934127
ISSN
1612-4758
DOI
10.1007/s10340-020-01250-6
language
English
LU publication?
yes
id
c55691b7-d86f-47bc-8c95-980b257536e5
date added to LUP
2020-07-13 11:32:30
date last changed
2021-01-06 03:47:50
@article{c55691b7-d86f-47bc-8c95-980b257536e5,
  abstract     = {<p>Pheromones are environmentally friendly alternatives to traditional pesticides for pest control. They are widely applied for insect monitoring, mating disruption and mass trapping. Nicotiana benthamiana and N. tabacum are potential green biomass production platforms of moth sex pheromones. Using these two Nicotiana species as plant factories, we expressed biosynthetic genes of plant and insect origin in leaf tissue. Moth sex pheromone precursors (E)-11-tetradecenoic acid, (Z)-11-tetradecenoic acid and (Z)-11-hexadecenoic acid were produced by introducing the acyl-ACP thioesterases CpuFatB1 from Cuphea pulcherrima or CpaFatB2 from C. palustris and the fatty acyl desaturases Ave∆11 from Argyrotaenia velutinana, CpaE11 from Choristoneura parallela or Atr∆11 from Amyelois transitella, under the control of CaMV-35S promoter. Among the Nicotiana spp. transformants, the best line produced (Z)-11-hexadecenoic acid at 17.6% of total fatty acids in leaves, during flowering stage, corresponding to 335 µg of (Z)-11-hexadecenoic acid per gram of fresh leaf. The (Z)-11-hexadecenoic acid production lines from N. benthamiana were selected for further propagation to obtain homozygous lines. In the N. benthamiana T2 generation, the production quantity of (Z)-11-hexadecenoic acid was stable. Our study demonstrates the feasibility of stable transformation of N. benthamiana for production of moth pheromone precursors in vegetative tissue.</p>},
  author       = {Xia, Yi Han and Ding, Bao Jian and Wang, Hong Lei and Hofvander, Per and Jarl-Sunesson, Carin and Löfstedt, Christer},
  issn         = {1612-4758},
  language     = {eng},
  number       = {4},
  pages        = {1333--1346},
  publisher    = {Springer},
  series       = {Journal of Pest Science},
  title        = {Production of moth sex pheromone precursors in Nicotiana spp. : a worthwhile new approach to pest control},
  url          = {http://dx.doi.org/10.1007/s10340-020-01250-6},
  doi          = {10.1007/s10340-020-01250-6},
  volume       = {93},
  year         = {2020},
}