Crystallization and preliminary X-ray analysis of three serotypes of foot-and-mouth disease virus
Curry, Stephen ; Abu-Ghazaleh, Robin ; Blakemore, Wendy ; Fry, Elizabeth ; Jackson, Terry ; King, Andrew ; Lea, Susan ; Logan, Derek LU
; Newman, John
and Stuart, David
(1992)
In Journal of Molecular Biology
228(4).
p.1263-1268
- Abstract
Foot-and-mouth disease viruses from serotypes O, A and C have been crystallized. The particular strains studied include O1K, A1061, A22 Iraq 24/64, A24 Cruzeiro and C-S8c1. In addition, crystals have been grown of G67, a monoclonal antibody neutralization escape mutant derived from O1K, and of virus R100, recovered after the establishment of a persistent infection in baby hamster kidney cells with C-S8c1. Empty particles, capsids which lack the RNA genome, have also been crystallized for subtypes A22 Iraq 24/64 and A1061. In almost all cases, crystals suitable for high resolution structure determination were obtained from (NH4)2S04 or mixtures of polyethylene... (More)
Foot-and-mouth disease viruses from serotypes O, A and C have been crystallized. The particular strains studied include O1K, A1061, A22 Iraq 24/64, A24 Cruzeiro and C-S8c1. In addition, crystals have been grown of G67, a monoclonal antibody neutralization escape mutant derived from O1K, and of virus R100, recovered after the establishment of a persistent infection in baby hamster kidney cells with C-S8c1. Empty particles, capsids which lack the RNA genome, have also been crystallized for subtypes A22 Iraq 24/64 and A1061. In almost all cases, crystals suitable for high resolution structure determination were obtained from (NH4)2S04 or mixtures of polyethylene glycol and NH4Cl.
(Less)
- author
- Curry, Stephen
; Abu-Ghazaleh, Robin
; Blakemore, Wendy
; Fry, Elizabeth
; Jackson, Terry
; King, Andrew
; Lea, Susan
; Logan, Derek
LU
; Newman, John
and Stuart, David
- publishing date
- 1992-12-20
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- crystallization, empty particles, foot-and-mouth disease virus, virus crystallography, X-ray diffraction
- in
- Journal of Molecular Biology
- volume
- 228
- issue
- 4
- pages
- 6 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:1335517
- scopus:0027080969
- ISSN
- 0022-2836
- DOI
- 10.1016/0022-2836(92)90332-E
- language
- English
- LU publication?
- no
- id
- c5584168-7def-449c-991d-0c465cd992eb
- date added to LUP
- 2022-04-25 11:34:32
- date last changed
- 2024-01-03 10:19:54
@article{c5584168-7def-449c-991d-0c465cd992eb,
abstract = {{<p>Foot-and-mouth disease viruses from serotypes O, A and C have been crystallized. The particular strains studied include O<sub>1</sub>K, A10<sub>61</sub>, A22 Iraq 24/64, A24 Cruzeiro and C-S8c1. In addition, crystals have been grown of G67, a monoclonal antibody neutralization escape mutant derived from O<sub>1</sub>K, and of virus R100, recovered after the establishment of a persistent infection in baby hamster kidney cells with C-S8c1. Empty particles, capsids which lack the RNA genome, have also been crystallized for subtypes A22 Iraq 24/64 and A10<sub>61</sub>. In almost all cases, crystals suitable for high resolution structure determination were obtained from (NH<sub>4</sub>)<sub>2</sub>S0<sub>4</sub> or mixtures of polyethylene glycol and NH<sub>4</sub>Cl.</p>}},
author = {{Curry, Stephen and Abu-Ghazaleh, Robin and Blakemore, Wendy and Fry, Elizabeth and Jackson, Terry and King, Andrew and Lea, Susan and Logan, Derek and Newman, John and Stuart, David}},
issn = {{0022-2836}},
keywords = {{crystallization; empty particles; foot-and-mouth disease virus; virus crystallography; X-ray diffraction}},
language = {{eng}},
month = {{12}},
number = {{4}},
pages = {{1263--1268}},
publisher = {{Elsevier}},
series = {{Journal of Molecular Biology}},
title = {{Crystallization and preliminary X-ray analysis of three serotypes of foot-and-mouth disease virus}},
url = {{http://dx.doi.org/10.1016/0022-2836(92)90332-E}},
doi = {{10.1016/0022-2836(92)90332-E}},
volume = {{228}},
year = {{1992}},
}