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Elevated CO2 reduces copper accumulation and toxicity in the diatom Thalassiosira pseudonana

Xu, Dong ; Huang, Shujie ; Fan, Xiao ; Zhang, Xiaowen ; Wang, Yitao ; Wang, Wei ; Beardall, John ; Brennan, Georgina LU and Ye, Naihao (2023) In Frontiers in Microbiology 13.
Abstract

The projected ocean acidification (OA) associated with increasing atmospheric CO2 alters seawater chemistry and hence the bio-toxicity of metal ions. However, it is still unclear how OA might affect the long-term resilience of globally important marine microalgae to anthropogenic metal stress. To explore the effect of increasing pCO2 on copper metabolism in the diatom Thalassiosira pseudonana (CCMP 1335), we employed an integrated eco-physiological, analytical chemistry, and transcriptomic approach to clarify the effect of increasing pCO2 on copper metabolism of Thalassiosira pseudonana across different temporal (short-term vs. long-term) and spatial (indoor laboratory experiments vs. outdoor mesocosms... (More)

The projected ocean acidification (OA) associated with increasing atmospheric CO2 alters seawater chemistry and hence the bio-toxicity of metal ions. However, it is still unclear how OA might affect the long-term resilience of globally important marine microalgae to anthropogenic metal stress. To explore the effect of increasing pCO2 on copper metabolism in the diatom Thalassiosira pseudonana (CCMP 1335), we employed an integrated eco-physiological, analytical chemistry, and transcriptomic approach to clarify the effect of increasing pCO2 on copper metabolism of Thalassiosira pseudonana across different temporal (short-term vs. long-term) and spatial (indoor laboratory experiments vs. outdoor mesocosms experiments) scales. We found that increasing pCO2 (1,000 and 2,000 μatm) promoted growth and photosynthesis, but decreased copper accumulation and alleviated its bio-toxicity to T. pseudonana. Transcriptomics results indicated that T. pseudonana altered the copper detoxification strategy under OA by decreasing copper uptake and enhancing copper-thiol complexation and copper efflux. Biochemical analysis further showed that the activities of the antioxidant enzymes glutathione peroxidase (GPX), catalase (CAT), and phytochelatin synthetase (PCS) were enhanced to mitigate oxidative damage of copper stress under elevated CO2. Our results provide a basis for a better understanding of the bioremediation capacity of marine primary producers, which may have profound effect on the security of seafood quality and marine ecosystem sustainability under further climate change.

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author
; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
adaptation, copper accumulation, copper toxicity, ocean acidification, Thalassiosira pseudonana
in
Frontiers in Microbiology
volume
13
article number
1113388
publisher
Frontiers Media S. A.
external identifiers
  • pmid:36687610
  • scopus:85146517377
ISSN
1664-302X
DOI
10.3389/fmicb.2022.1113388
language
English
LU publication?
no
additional info
Publisher Copyright: Copyright © 2023 Xu, Huang, Fan, Zhang, Wang, Wang, Beardall, Brennan and Ye.
id
c58ebf59-801d-4aa6-9f55-06450dc7e915
date added to LUP
2024-10-15 14:48:51
date last changed
2025-07-23 14:35:05
@article{c58ebf59-801d-4aa6-9f55-06450dc7e915,
  abstract     = {{<p>The projected ocean acidification (OA) associated with increasing atmospheric CO<sub>2</sub> alters seawater chemistry and hence the bio-toxicity of metal ions. However, it is still unclear how OA might affect the long-term resilience of globally important marine microalgae to anthropogenic metal stress. To explore the effect of increasing pCO<sub>2</sub> on copper metabolism in the diatom Thalassiosira pseudonana (CCMP 1335), we employed an integrated eco-physiological, analytical chemistry, and transcriptomic approach to clarify the effect of increasing pCO<sub>2</sub> on copper metabolism of Thalassiosira pseudonana across different temporal (short-term vs. long-term) and spatial (indoor laboratory experiments vs. outdoor mesocosms experiments) scales. We found that increasing pCO<sub>2</sub> (1,000 and 2,000 μatm) promoted growth and photosynthesis, but decreased copper accumulation and alleviated its bio-toxicity to T. pseudonana. Transcriptomics results indicated that T. pseudonana altered the copper detoxification strategy under OA by decreasing copper uptake and enhancing copper-thiol complexation and copper efflux. Biochemical analysis further showed that the activities of the antioxidant enzymes glutathione peroxidase (GPX), catalase (CAT), and phytochelatin synthetase (PCS) were enhanced to mitigate oxidative damage of copper stress under elevated CO<sub>2</sub>. Our results provide a basis for a better understanding of the bioremediation capacity of marine primary producers, which may have profound effect on the security of seafood quality and marine ecosystem sustainability under further climate change.</p>}},
  author       = {{Xu, Dong and Huang, Shujie and Fan, Xiao and Zhang, Xiaowen and Wang, Yitao and Wang, Wei and Beardall, John and Brennan, Georgina and Ye, Naihao}},
  issn         = {{1664-302X}},
  keywords     = {{adaptation; copper accumulation; copper toxicity; ocean acidification; Thalassiosira pseudonana}},
  language     = {{eng}},
  month        = {{01}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Microbiology}},
  title        = {{Elevated CO<sub>2</sub> reduces copper accumulation and toxicity in the diatom Thalassiosira pseudonana}},
  url          = {{http://dx.doi.org/10.3389/fmicb.2022.1113388}},
  doi          = {{10.3389/fmicb.2022.1113388}},
  volume       = {{13}},
  year         = {{2023}},
}