Serum and gene expression levels of CT-1, IL-6, and TNF-α after a lifestyle intervention in obese children
(2018) In Pediatric Diabetes 19(2). p.217-222- Abstract
Background: Inflammation related molecules such as tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and cardiotrophin-1 (CT-1) are highly expressed in obese individuals and could partly explain some comorbidities associated to obesity. In obese children, lifestyle interventions are able to lower inflammation and reduce cardiovascular risk factors associated with obesity. The aim of the present work was to study changes in inflammation-related molecules serum and peripheral blood mononuclear cells (PBMC) transcript levels after a 10-week lifestyle intervention in obese children and asses their potential association with glucose metabolism. Methods: Twenty-three obese children (mean age 11.5 years; 48% males) underwent a 10-week... (More)
Background: Inflammation related molecules such as tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and cardiotrophin-1 (CT-1) are highly expressed in obese individuals and could partly explain some comorbidities associated to obesity. In obese children, lifestyle interventions are able to lower inflammation and reduce cardiovascular risk factors associated with obesity. The aim of the present work was to study changes in inflammation-related molecules serum and peripheral blood mononuclear cells (PBMC) transcript levels after a 10-week lifestyle intervention in obese children and asses their potential association with glucose metabolism. Methods: Twenty-three obese children (mean age 11.5 years; 48% males) underwent a 10-week lifestyle not controlled intervention trial. Anthropometric and biochemical measurements were analyzed. Transcript analysis for CT-1, IL-6, and TNF-α in PBMC were performed by RT-PCR. Serum cytokine levels were also measured at baseline and after 10-weeks. Results: Participants achieved a significant reduction in body adiposity (0.34 decrease in body mass index-standard deviation), total cholesterol, and glucose levels after 10-weeks. A Significant decrease in serum TNF-α and C reactive protein (CRP) were observed. CT-1 transcript levels were significantly reduced (P =.005) after lifestyle intervention, and these changes were significantly correlated with changes in serum CT-1 levels (r = 0.451; P =.031). In multiple regression analysis baseline CT-1 transcript levels were positively associated with final insulin (R2 = 0.506; P =.035) and HOMA-IR values (R2 = 0.473; P =.034). Conclusions: We reported that serum CRP, TNF-α, as well as PBMC CT-1 transcript levels were reduced after lifestyle intervention in obese children. More studies are needed to clarify the role of inflammation-related molecules in glucose metabolism.
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- author
- Marti, A. ; Morell-Azanza, L. ; Rendo-Urteaga, T. ; García-Calzón, S. LU ; Ojeda-Rodríguez, A. ; Martín-Calvo, N. ; Moreno-Aliaga, M. J. ; Martínez, J. A. and Azcona-San Julián, M. C.
- organization
- publishing date
- 2018-03
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- childhood obesity, CT-1, gene expression, inflammation markers, lifestyle intervention, weight loss
- in
- Pediatric Diabetes
- volume
- 19
- issue
- 2
- pages
- 6 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:28749076
- scopus:85041966310
- ISSN
- 1399-543X
- DOI
- 10.1111/pedi.12561
- language
- English
- LU publication?
- yes
- id
- c5a8087b-a09a-4d41-9bf3-1af5df7bf029
- date added to LUP
- 2018-02-20 14:31:29
- date last changed
- 2024-03-18 05:20:29
@article{c5a8087b-a09a-4d41-9bf3-1af5df7bf029,
abstract = {{<p>Background: Inflammation related molecules such as tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and cardiotrophin-1 (CT-1) are highly expressed in obese individuals and could partly explain some comorbidities associated to obesity. In obese children, lifestyle interventions are able to lower inflammation and reduce cardiovascular risk factors associated with obesity. The aim of the present work was to study changes in inflammation-related molecules serum and peripheral blood mononuclear cells (PBMC) transcript levels after a 10-week lifestyle intervention in obese children and asses their potential association with glucose metabolism. Methods: Twenty-three obese children (mean age 11.5 years; 48% males) underwent a 10-week lifestyle not controlled intervention trial. Anthropometric and biochemical measurements were analyzed. Transcript analysis for CT-1, IL-6, and TNF-α in PBMC were performed by RT-PCR. Serum cytokine levels were also measured at baseline and after 10-weeks. Results: Participants achieved a significant reduction in body adiposity (0.34 decrease in body mass index-standard deviation), total cholesterol, and glucose levels after 10-weeks. A Significant decrease in serum TNF-α and C reactive protein (CRP) were observed. CT-1 transcript levels were significantly reduced (P =.005) after lifestyle intervention, and these changes were significantly correlated with changes in serum CT-1 levels (r = 0.451; P =.031). In multiple regression analysis baseline CT-1 transcript levels were positively associated with final insulin (R<sup>2</sup> = 0.506; P =.035) and HOMA-IR values (R<sup>2</sup> = 0.473; P =.034). Conclusions: We reported that serum CRP, TNF-α, as well as PBMC CT-1 transcript levels were reduced after lifestyle intervention in obese children. More studies are needed to clarify the role of inflammation-related molecules in glucose metabolism.</p>}},
author = {{Marti, A. and Morell-Azanza, L. and Rendo-Urteaga, T. and García-Calzón, S. and Ojeda-Rodríguez, A. and Martín-Calvo, N. and Moreno-Aliaga, M. J. and Martínez, J. A. and Azcona-San Julián, M. C.}},
issn = {{1399-543X}},
keywords = {{childhood obesity; CT-1; gene expression; inflammation markers; lifestyle intervention; weight loss}},
language = {{eng}},
number = {{2}},
pages = {{217--222}},
publisher = {{Wiley-Blackwell}},
series = {{Pediatric Diabetes}},
title = {{Serum and gene expression levels of CT-1, IL-6, and TNF-α after a lifestyle intervention in obese children}},
url = {{http://dx.doi.org/10.1111/pedi.12561}},
doi = {{10.1111/pedi.12561}},
volume = {{19}},
year = {{2018}},
}