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Serum and gene expression levels of CT-1, IL-6, and TNF-α after a lifestyle intervention in obese children

Marti, A.; Morell-Azanza, L.; Rendo-Urteaga, T.; García-Calzón, S. LU ; Ojeda-Rodríguez, A.; Martín-Calvo, N.; Moreno-Aliaga, M. J.; Martínez, J. A. and Azcona-San Julián, M. C. (2018) In Pediatric Diabetes 19(2). p.217-222
Abstract

Background: Inflammation related molecules such as tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and cardiotrophin-1 (CT-1) are highly expressed in obese individuals and could partly explain some comorbidities associated to obesity. In obese children, lifestyle interventions are able to lower inflammation and reduce cardiovascular risk factors associated with obesity. The aim of the present work was to study changes in inflammation-related molecules serum and peripheral blood mononuclear cells (PBMC) transcript levels after a 10-week lifestyle intervention in obese children and asses their potential association with glucose metabolism. Methods: Twenty-three obese children (mean age 11.5 years; 48% males) underwent a 10-week... (More)

Background: Inflammation related molecules such as tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and cardiotrophin-1 (CT-1) are highly expressed in obese individuals and could partly explain some comorbidities associated to obesity. In obese children, lifestyle interventions are able to lower inflammation and reduce cardiovascular risk factors associated with obesity. The aim of the present work was to study changes in inflammation-related molecules serum and peripheral blood mononuclear cells (PBMC) transcript levels after a 10-week lifestyle intervention in obese children and asses their potential association with glucose metabolism. Methods: Twenty-three obese children (mean age 11.5 years; 48% males) underwent a 10-week lifestyle not controlled intervention trial. Anthropometric and biochemical measurements were analyzed. Transcript analysis for CT-1, IL-6, and TNF-α in PBMC were performed by RT-PCR. Serum cytokine levels were also measured at baseline and after 10-weeks. Results: Participants achieved a significant reduction in body adiposity (0.34 decrease in body mass index-standard deviation), total cholesterol, and glucose levels after 10-weeks. A Significant decrease in serum TNF-α and C reactive protein (CRP) were observed. CT-1 transcript levels were significantly reduced (P =.005) after lifestyle intervention, and these changes were significantly correlated with changes in serum CT-1 levels (r = 0.451; P =.031). In multiple regression analysis baseline CT-1 transcript levels were positively associated with final insulin (R2 = 0.506; P =.035) and HOMA-IR values (R2 = 0.473; P =.034). Conclusions: We reported that serum CRP, TNF-α, as well as PBMC CT-1 transcript levels were reduced after lifestyle intervention in obese children. More studies are needed to clarify the role of inflammation-related molecules in glucose metabolism.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
childhood obesity, CT-1, gene expression, inflammation markers, lifestyle intervention, weight loss
in
Pediatric Diabetes
volume
19
issue
2
pages
6 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:85041966310
ISSN
1399-543X
DOI
10.1111/pedi.12561
language
English
LU publication?
yes
id
c5a8087b-a09a-4d41-9bf3-1af5df7bf029
date added to LUP
2018-02-20 14:31:29
date last changed
2018-05-29 11:10:01
@article{c5a8087b-a09a-4d41-9bf3-1af5df7bf029,
  abstract     = {<p>Background: Inflammation related molecules such as tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and cardiotrophin-1 (CT-1) are highly expressed in obese individuals and could partly explain some comorbidities associated to obesity. In obese children, lifestyle interventions are able to lower inflammation and reduce cardiovascular risk factors associated with obesity. The aim of the present work was to study changes in inflammation-related molecules serum and peripheral blood mononuclear cells (PBMC) transcript levels after a 10-week lifestyle intervention in obese children and asses their potential association with glucose metabolism. Methods: Twenty-three obese children (mean age 11.5 years; 48% males) underwent a 10-week lifestyle not controlled intervention trial. Anthropometric and biochemical measurements were analyzed. Transcript analysis for CT-1, IL-6, and TNF-α in PBMC were performed by RT-PCR. Serum cytokine levels were also measured at baseline and after 10-weeks. Results: Participants achieved a significant reduction in body adiposity (0.34 decrease in body mass index-standard deviation), total cholesterol, and glucose levels after 10-weeks. A Significant decrease in serum TNF-α and C reactive protein (CRP) were observed. CT-1 transcript levels were significantly reduced (P =.005) after lifestyle intervention, and these changes were significantly correlated with changes in serum CT-1 levels (r = 0.451; P =.031). In multiple regression analysis baseline CT-1 transcript levels were positively associated with final insulin (R<sup>2</sup> = 0.506; P =.035) and HOMA-IR values (R<sup>2</sup> = 0.473; P =.034). Conclusions: We reported that serum CRP, TNF-α, as well as PBMC CT-1 transcript levels were reduced after lifestyle intervention in obese children. More studies are needed to clarify the role of inflammation-related molecules in glucose metabolism.</p>},
  author       = {Marti, A. and Morell-Azanza, L. and Rendo-Urteaga, T. and García-Calzón, S. and Ojeda-Rodríguez, A. and Martín-Calvo, N. and Moreno-Aliaga, M. J. and Martínez, J. A. and Azcona-San Julián, M. C.},
  issn         = {1399-543X},
  keyword      = {childhood obesity,CT-1,gene expression,inflammation markers,lifestyle intervention,weight loss},
  language     = {eng},
  number       = {2},
  pages        = {217--222},
  publisher    = {Wiley-Blackwell},
  series       = {Pediatric Diabetes},
  title        = {Serum and gene expression levels of CT-1, IL-6, and TNF-α after a lifestyle intervention in obese children},
  url          = {http://dx.doi.org/10.1111/pedi.12561},
  volume       = {19},
  year         = {2018},
}