氨曲南竞争性抑制NDM-1对β-内酰胺类抗生素的水解
(2019) In Journal of Shanxi University (Natural Science Edition)- Abstract
- We investigated the effect of Aztreonam on hydrolysis of β-lactam antibiotics by MBL using New Delhi Metallo-β-lactamase-1(NDM-1) as a model. The results showed that Aztreonam significantly inhibited hydrolysis of Nitrocefin and Meropenem by soluble NDM-1, but also inhibited hydrolysis of Penicillin G by CPG beads immobilized NDM-1(NDM-1 beads). Moreover, in spite of extensive washing for multiple times, the activity to hydrolyze Penicillin G by the Aztreoman pre-treated NDM-1 beads was just partially recovered. These data suggest that Aztreonam can covalently and stably bind on NDM-1, thus efficiently inhibiting hydrolysis of other kinds of β-lactam antibiotics by NDM-1 in a competitive way.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/c5d81e0e-796d-480b-91cf-6156ad1e2b73
- author
- Wang, Xi ; Li, Binchun ; Zhang, Sheng ; Xie, Bin LU ; Wu, Changxin ; Li, Shenwei and Meng, Qinglai
- organization
- alternative title
- Aztreonam Competitively Inhibits Hydrolysis of β-Lactam Antibiotics by NDM-1
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aztreonam, NDM-1, metal-β-lactamase, Superbug, Antimicrobial resistance
- in
- Journal of Shanxi University (Natural Science Edition)
- issue
- 2021-01
- language
- Chinese (Simplified)
- LU publication?
- yes
- id
- c5d81e0e-796d-480b-91cf-6156ad1e2b73
- alternative location
- http://cnki.nbsti.net/KCMS/detail/detail.aspx?filename=SXDR202101023&dbcode=CJFD&dbname=CJFD2021
- date added to LUP
- 2021-10-26 19:42:42
- date last changed
- 2021-11-02 09:30:33
@article{c5d81e0e-796d-480b-91cf-6156ad1e2b73, abstract = {{We investigated the effect of Aztreonam on hydrolysis of β-lactam antibiotics by MBL using New Delhi Metallo-β-lactamase-1(NDM-1) as a model. The results showed that Aztreonam significantly inhibited hydrolysis of Nitrocefin and Meropenem by soluble NDM-1, but also inhibited hydrolysis of Penicillin G by CPG beads immobilized NDM-1(NDM-1 beads). Moreover, in spite of extensive washing for multiple times, the activity to hydrolyze Penicillin G by the Aztreoman pre-treated NDM-1 beads was just partially recovered. These data suggest that Aztreonam can covalently and stably bind on NDM-1, thus efficiently inhibiting hydrolysis of other kinds of β-lactam antibiotics by NDM-1 in a competitive way.}}, author = {{Wang, Xi and Li, Binchun and Zhang, Sheng and Xie, Bin and Wu, Changxin and Li, Shenwei and Meng, Qinglai}}, keywords = {{Aztreonam; NDM-1; metal-β-lactamase; Superbug; Antimicrobial resistance}}, language = {{chi}}, number = {{2021-01}}, series = {{Journal of Shanxi University (Natural Science Edition)}}, title = {{氨曲南竞争性抑制NDM-1对β-内酰胺类抗生素的水解}}, url = {{http://cnki.nbsti.net/KCMS/detail/detail.aspx?filename=SXDR202101023&dbcode=CJFD&dbname=CJFD2021}}, year = {{2019}}, }