Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

氨曲南竞争性抑制NDM-1对β-内酰胺类抗生素的水解

Wang, Xi ; Li, Binchun ; Zhang, Sheng ; Xie, Bin LU ; Wu, Changxin ; Li, Shenwei and Meng, Qinglai (2019) In Journal of Shanxi University (Natural Science Edition)
Abstract
We investigated the effect of Aztreonam on hydrolysis of β-lactam antibiotics by MBL using New Delhi Metallo-β-lactamase-1(NDM-1) as a model. The results showed that Aztreonam significantly inhibited hydrolysis of Nitrocefin and Meropenem by soluble NDM-1, but also inhibited hydrolysis of Penicillin G by CPG beads immobilized NDM-1(NDM-1 beads). Moreover, in spite of extensive washing for multiple times, the activity to hydrolyze Penicillin G by the Aztreoman pre-treated NDM-1 beads was just partially recovered. These data suggest that Aztreonam can covalently and stably bind on NDM-1, thus efficiently inhibiting hydrolysis of other kinds of β-lactam antibiotics by NDM-1 in a competitive way.
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
organization
alternative title
Aztreonam Competitively Inhibits Hydrolysis of β-Lactam Antibiotics by NDM-1
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aztreonam, NDM-1, metal-β-lactamase, Superbug, Antimicrobial resistance
in
Journal of Shanxi University (Natural Science Edition)
issue
2021-01
language
Chinese (Simplified)
LU publication?
yes
id
c5d81e0e-796d-480b-91cf-6156ad1e2b73
alternative location
http://cnki.nbsti.net/KCMS/detail/detail.aspx?filename=SXDR202101023&dbcode=CJFD&dbname=CJFD2021
date added to LUP
2021-10-26 19:42:42
date last changed
2021-11-02 09:30:33
@article{c5d81e0e-796d-480b-91cf-6156ad1e2b73,
  abstract     = {{We investigated the effect of Aztreonam on hydrolysis of β-lactam antibiotics by MBL using New Delhi Metallo-β-lactamase-1(NDM-1) as a model. The results showed that Aztreonam significantly inhibited hydrolysis of Nitrocefin and Meropenem by soluble NDM-1, but also inhibited hydrolysis of Penicillin G by CPG beads immobilized NDM-1(NDM-1 beads). Moreover, in spite of extensive washing for multiple times, the activity to hydrolyze Penicillin G by the Aztreoman pre-treated NDM-1 beads was just partially recovered. These data suggest that Aztreonam can covalently and stably bind on NDM-1, thus efficiently inhibiting hydrolysis of other kinds of β-lactam antibiotics by NDM-1 in a competitive way.}},
  author       = {{Wang, Xi and Li, Binchun and Zhang, Sheng and Xie, Bin and Wu, Changxin and Li, Shenwei and Meng, Qinglai}},
  keywords     = {{Aztreonam; NDM-1; metal-β-lactamase; Superbug; Antimicrobial resistance}},
  language     = {{chi}},
  number       = {{2021-01}},
  series       = {{Journal of Shanxi University (Natural Science Edition)}},
  title        = {{氨曲南竞争性抑制NDM-1对β-内酰胺类抗生素的水解}},
  url          = {{http://cnki.nbsti.net/KCMS/detail/detail.aspx?filename=SXDR202101023&dbcode=CJFD&dbname=CJFD2021}},
  year         = {{2019}},
}