Telomerase in yeast
(1995) In Science 269(5222). p.396-400- Abstract
- The ribonucleoprotein enzyme telomerase synthesizes telomeric DNA by copying an internal RNA template sequence. The telomerase activities of the yeasts Saccharomyces castellii and Saccharomyces cerevisiae-with regular and irregular telomeric sequences, respectively-have now been identified and characterized. The S. cerevisiae activity required the telomerase RNA gene TLC1 but not the EST1 gene, both of which are required for normal telomere maintenance in vivo. This activity exhibited low processivity and produced no regularly repeated products. An inherently high stalling frequency of the S. cerevisiae telomerase may account for its in vitro properties and for the irregular telomeric sequences of this yeast.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4754351
- author
- Cohn, Marita LU and Blackburn, E.H.
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Science
- volume
- 269
- issue
- 5222
- pages
- 396 - 400
- publisher
- American Association for the Advancement of Science (AAAS)
- external identifiers
-
- scopus:0029155795
- ISSN
- 1095-9203
- DOI
- 10.1126/science.7618104
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Genetics (Closed 2011) (011005100)
- id
- c5f8eb2a-8720-4002-80f7-eba064104149 (old id 4754351)
- date added to LUP
- 2016-04-01 16:53:37
- date last changed
- 2021-09-26 03:17:21
@article{c5f8eb2a-8720-4002-80f7-eba064104149, abstract = {{The ribonucleoprotein enzyme telomerase synthesizes telomeric DNA by copying an internal RNA template sequence. The telomerase activities of the yeasts Saccharomyces castellii and Saccharomyces cerevisiae-with regular and irregular telomeric sequences, respectively-have now been identified and characterized. The S. cerevisiae activity required the telomerase RNA gene TLC1 but not the EST1 gene, both of which are required for normal telomere maintenance in vivo. This activity exhibited low processivity and produced no regularly repeated products. An inherently high stalling frequency of the S. cerevisiae telomerase may account for its in vitro properties and for the irregular telomeric sequences of this yeast.}}, author = {{Cohn, Marita and Blackburn, E.H.}}, issn = {{1095-9203}}, language = {{eng}}, number = {{5222}}, pages = {{396--400}}, publisher = {{American Association for the Advancement of Science (AAAS)}}, series = {{Science}}, title = {{Telomerase in yeast}}, url = {{http://dx.doi.org/10.1126/science.7618104}}, doi = {{10.1126/science.7618104}}, volume = {{269}}, year = {{1995}}, }