Effects of ginkgolide b, a platelet-activating factor inhibitor on insulitis in the spontaneously diabetic bb rat
Beck, Jill C. ; Goodner, Charles J. ; Wilson, Cindy ; Wilson, Deborah ; Glidden, Dave ; Baskin, Denis G. ; Lernmark, Åke LU
and Braquet, Pierre
(1991)
In Autoimmunity
9(3).
p.225-235
- Abstract
The BB rat spontaneously develops insulin-dependent diabetes mellitus (IDDM) in association with marked insulitis in the islets of Langerhans. Since platelet-activating factor (PAF-acether) is involved in allergic and inflammatory reactions, we tested a PAF antagonist, Ginkgolide B (BN 52021) for potential effects on islet inflammation and diabetes. Diabetes prone BB/Wor rats were treated daily from weaning at 25 days until 105 days of age with either saline (n = 30, controls), 10 (n = 25, low dose) or 20 (n = 30, high dose) mg/kg body weight of BN 52021. The overall incidence of IDDM was unaffected by treatment. Quantitative analysis of insulin area showed a dose-dependent protection of β cells by Ginkgolide B, reflected in a 6- (low... (More)
The BB rat spontaneously develops insulin-dependent diabetes mellitus (IDDM) in association with marked insulitis in the islets of Langerhans. Since platelet-activating factor (PAF-acether) is involved in allergic and inflammatory reactions, we tested a PAF antagonist, Ginkgolide B (BN 52021) for potential effects on islet inflammation and diabetes. Diabetes prone BB/Wor rats were treated daily from weaning at 25 days until 105 days of age with either saline (n = 30, controls), 10 (n = 25, low dose) or 20 (n = 30, high dose) mg/kg body weight of BN 52021. The overall incidence of IDDM was unaffected by treatment. Quantitative analysis of insulin area showed a dose-dependent protection of β cells by Ginkgolide B, reflected in a 6- (low dose) to 8-fold (high dose) (P < 0. 01-0. 005) increase in the insulin/glucagon cell ratio compared to the saline treated rats. Ginkgolide B reduced severe insulitis from 84% in the saline rats developing IDDM to 59% (n. s.) in the low and to 33% (P < 0. 001) in the high dose group. These data suggest that PAF inhibitors may prove useful in immunomodulator therapy of IDDM since β cells are preserved.
(Less)
- author
- Beck, Jill C.
; Goodner, Charles J.
; Wilson, Cindy
; Wilson, Deborah
; Glidden, Dave
; Baskin, Denis G.
; Lernmark, Åke
LU
and Braquet, Pierre
- publishing date
- 1991-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Autoimmunity, Diabetes mellitus, Eosinophils, Glucagon, Insulin, Morphometry
- in
- Autoimmunity
- volume
- 9
- issue
- 3
- pages
- 11 pages
- publisher
- Taylor & Francis
- external identifiers
-
- pmid:1777555
- scopus:0025818191
- ISSN
- 0891-6934
- DOI
- 10.3109/08916939109007648
- language
- English
- LU publication?
- no
- id
- c624feb3-56f9-4f66-9093-5f08791991b5
- date added to LUP
- 2019-09-11 09:40:24
- date last changed
- 2024-03-13 08:02:32
@article{c624feb3-56f9-4f66-9093-5f08791991b5,
abstract = {{<p>The BB rat spontaneously develops insulin-dependent diabetes mellitus (IDDM) in association with marked insulitis in the islets of Langerhans. Since platelet-activating factor (PAF-acether) is involved in allergic and inflammatory reactions, we tested a PAF antagonist, Ginkgolide B (BN 52021) for potential effects on islet inflammation and diabetes. Diabetes prone BB/Wor rats were treated daily from weaning at 25 days until 105 days of age with either saline (n = 30, controls), 10 (n = 25, low dose) or 20 (n = 30, high dose) mg/kg body weight of BN 52021. The overall incidence of IDDM was unaffected by treatment. Quantitative analysis of insulin area showed a dose-dependent protection of β cells by Ginkgolide B, reflected in a 6- (low dose) to 8-fold (high dose) (P < 0. 01-0. 005) increase in the insulin/glucagon cell ratio compared to the saline treated rats. Ginkgolide B reduced severe insulitis from 84% in the saline rats developing IDDM to 59% (n. s.) in the low and to 33% (P < 0. 001) in the high dose group. These data suggest that PAF inhibitors may prove useful in immunomodulator therapy of IDDM since β cells are preserved.</p>}},
author = {{Beck, Jill C. and Goodner, Charles J. and Wilson, Cindy and Wilson, Deborah and Glidden, Dave and Baskin, Denis G. and Lernmark, Åke and Braquet, Pierre}},
issn = {{0891-6934}},
keywords = {{Autoimmunity; Diabetes mellitus; Eosinophils; Glucagon; Insulin; Morphometry}},
language = {{eng}},
month = {{01}},
number = {{3}},
pages = {{225--235}},
publisher = {{Taylor & Francis}},
series = {{Autoimmunity}},
title = {{Effects of ginkgolide b, a platelet-activating factor inhibitor on insulitis in the spontaneously diabetic bb rat}},
url = {{http://dx.doi.org/10.3109/08916939109007648}},
doi = {{10.3109/08916939109007648}},
volume = {{9}},
year = {{1991}},
}