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Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry

Sihlbom, Carina ; Davidsson, Pia and Nilsson, Carol L LU (2005) In Journal of Proteome Research 4(6). p.301-2294
Abstract

Glycoproteins in cerebrospinal fluid are found to be altered in Alzheimer patients compared to healthy control individuals. We have utilized micro-solution isoelectric focusing and affinity chromatography, prior to gel electrophoresis to enable site-specific structural determination of the N-linked glycans in apolipoprotein J with the use of FT-ICR MS. The albumin depletion method is the most suitable as prefractionation method of CSF prior to 2-DE for structural determination of glycoproteins in the study of neurodegenerative disorders.

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author
; and
publishing date
type
Contribution to journal
publication status
published
keywords
Albumins, Alzheimer Disease, Cerebrospinal Fluid, Chromatography, Affinity, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Glycopeptides, Glycoproteins, Humans, Hydrogen-Ion Concentration, Isoelectric Focusing, Mass Spectrometry, Polysaccharides, Protein Isoforms, Spectroscopy, Fourier Transform Infrared, Trypsin, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Proteome Research
volume
4
issue
6
pages
8 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:16335978
  • scopus:29144502243
ISSN
1535-3893
DOI
10.1021/pr050210g
language
English
LU publication?
no
id
c6550760-89e5-47ff-a667-513ff4fc646d
date added to LUP
2017-05-16 10:35:59
date last changed
2024-10-14 06:12:25
@article{c6550760-89e5-47ff-a667-513ff4fc646d,
  abstract     = {{<p>Glycoproteins in cerebrospinal fluid are found to be altered in Alzheimer patients compared to healthy control individuals. We have utilized micro-solution isoelectric focusing and affinity chromatography, prior to gel electrophoresis to enable site-specific structural determination of the N-linked glycans in apolipoprotein J with the use of FT-ICR MS. The albumin depletion method is the most suitable as prefractionation method of CSF prior to 2-DE for structural determination of glycoproteins in the study of neurodegenerative disorders.</p>}},
  author       = {{Sihlbom, Carina and Davidsson, Pia and Nilsson, Carol L}},
  issn         = {{1535-3893}},
  keywords     = {{Albumins; Alzheimer Disease; Cerebrospinal Fluid; Chromatography, Affinity; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Glycopeptides; Glycoproteins; Humans; Hydrogen-Ion Concentration; Isoelectric Focusing; Mass Spectrometry; Polysaccharides; Protein Isoforms; Spectroscopy, Fourier Transform Infrared; Trypsin; Journal Article; Research Support, Non-U.S. Gov't}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{6}},
  pages        = {{301--2294}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Journal of Proteome Research}},
  title        = {{Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry}},
  url          = {{http://dx.doi.org/10.1021/pr050210g}},
  doi          = {{10.1021/pr050210g}},
  volume       = {{4}},
  year         = {{2005}},
}