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Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry

Sihlbom, Carina; Davidsson, Pia and Nilsson, Carol L LU (2005) In Journal of Proteome Research 4(6). p.301-2294
Abstract

Glycoproteins in cerebrospinal fluid are found to be altered in Alzheimer patients compared to healthy control individuals. We have utilized micro-solution isoelectric focusing and affinity chromatography, prior to gel electrophoresis to enable site-specific structural determination of the N-linked glycans in apolipoprotein J with the use of FT-ICR MS. The albumin depletion method is the most suitable as prefractionation method of CSF prior to 2-DE for structural determination of glycoproteins in the study of neurodegenerative disorders.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Albumins, Alzheimer Disease, Cerebrospinal Fluid, Chromatography, Affinity, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Glycopeptides, Glycoproteins, Humans, Hydrogen-Ion Concentration, Isoelectric Focusing, Mass Spectrometry, Polysaccharides, Protein Isoforms, Spectroscopy, Fourier Transform Infrared, Trypsin, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Proteome Research
volume
4
issue
6
pages
8 pages
publisher
The American Chemical Society
external identifiers
  • scopus:29144502243
ISSN
1535-3893
DOI
10.1021/pr050210g
language
English
LU publication?
no
id
c6550760-89e5-47ff-a667-513ff4fc646d
date added to LUP
2017-05-16 10:35:59
date last changed
2017-05-21 05:01:38
@article{c6550760-89e5-47ff-a667-513ff4fc646d,
  abstract     = {<p>Glycoproteins in cerebrospinal fluid are found to be altered in Alzheimer patients compared to healthy control individuals. We have utilized micro-solution isoelectric focusing and affinity chromatography, prior to gel electrophoresis to enable site-specific structural determination of the N-linked glycans in apolipoprotein J with the use of FT-ICR MS. The albumin depletion method is the most suitable as prefractionation method of CSF prior to 2-DE for structural determination of glycoproteins in the study of neurodegenerative disorders.</p>},
  author       = {Sihlbom, Carina and Davidsson, Pia and Nilsson, Carol L},
  issn         = {1535-3893},
  keyword      = {Albumins,Alzheimer Disease,Cerebrospinal Fluid,Chromatography, Affinity,Electrophoresis, Gel, Two-Dimensional,Electrophoresis, Polyacrylamide Gel,Glycopeptides,Glycoproteins,Humans,Hydrogen-Ion Concentration,Isoelectric Focusing,Mass Spectrometry,Polysaccharides,Protein Isoforms,Spectroscopy, Fourier Transform Infrared,Trypsin,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {12},
  number       = {6},
  pages        = {301--2294},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry},
  url          = {http://dx.doi.org/10.1021/pr050210g},
  volume       = {4},
  year         = {2005},
}