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Development of phospholipase A2 and lysophosphatidylcholine metabolising enzyme activities in the neonatal rat intestine

Tagesson, C. ; Telemo, E. ; Ekstrom, G. and Westrom, B. LU (1987) In Gut 28(7). p.822-828
Abstract

We have studied the development of phospholipase A2 (PLA2) and lysophosphatidylcholine (lysoPC)-metabolising enzyme activities in the neonatal rat intestine and its relation to the intestinal permeability of macromolecules. The permeability was determined by feeding young rats a mixture of bovine serum albumin, bovine immunoglobulin G and fluorescein-isothiocyanate-conjugated dextran 70,000, and analysing the serum concentrations after six hours. The animals were then killed and the intestinal mucosa was homogenised and assessed for PLA2 and lysoPC-metabolising enzyme activities. The intestine was 'open' to the macromolecules in 14 day old animals, but 'closed' in 22 and 32 day old animals and in 14 day... (More)

We have studied the development of phospholipase A2 (PLA2) and lysophosphatidylcholine (lysoPC)-metabolising enzyme activities in the neonatal rat intestine and its relation to the intestinal permeability of macromolecules. The permeability was determined by feeding young rats a mixture of bovine serum albumin, bovine immunoglobulin G and fluorescein-isothiocyanate-conjugated dextran 70,000, and analysing the serum concentrations after six hours. The animals were then killed and the intestinal mucosa was homogenised and assessed for PLA2 and lysoPC-metabolising enzyme activities. The intestine was 'open' to the macromolecules in 14 day old animals, but 'closed' in 22 and 32 day old animals and in 14 day old rats treated with cortisone acetate on day 10, 11, and 12 postpartum. The activity of PLA2 (at pH 6 and 2 mM Ca2+) was higher in 32, 22, and cortisone treated 14 day old animals, than in untreated, 14 day old animals. Incubation of 14C-acyl-lysoPC mucosa from 14 day old rats did not change the radioactivity pattern as shown by thin layer chromatography, whereas after incubation with mucosa from 22 or 32 day old animals all the radiolabel was found in free 14C-fatty acid and in 14C-phosphatidylcholine. These findings indicate that mucosal PLA2 activity increases during intestinal maturation and that the mucosa acquires the ability to acylate and deacylate lysoPC when it is 'closed' to macromolecules.

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publication status
published
subject
in
Gut
volume
28
issue
7
pages
7 pages
publisher
BMJ Publishing Group
external identifiers
  • pmid:2443432
  • scopus:0023162492
ISSN
0017-5749
DOI
10.1136/gut.28.7.822
language
English
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yes
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c68c03a4-e587-42cb-bd68-370687e3358b
date added to LUP
2024-12-05 15:33:02
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@article{c68c03a4-e587-42cb-bd68-370687e3358b,
  abstract     = {{<p>We have studied the development of phospholipase A<sub>2</sub> (PLA<sub>2</sub>) and lysophosphatidylcholine (lysoPC)-metabolising enzyme activities in the neonatal rat intestine and its relation to the intestinal permeability of macromolecules. The permeability was determined by feeding young rats a mixture of bovine serum albumin, bovine immunoglobulin G and fluorescein-isothiocyanate-conjugated dextran 70,000, and analysing the serum concentrations after six hours. The animals were then killed and the intestinal mucosa was homogenised and assessed for PLA<sub>2</sub> and lysoPC-metabolising enzyme activities. The intestine was 'open' to the macromolecules in 14 day old animals, but 'closed' in 22 and 32 day old animals and in 14 day old rats treated with cortisone acetate on day 10, 11, and 12 postpartum. The activity of PLA<sub>2</sub> (at pH 6 and 2 mM Ca<sup>2+</sup>) was higher in 32, 22, and cortisone treated 14 day old animals, than in untreated, 14 day old animals. Incubation of <sup>14</sup>C-acyl-lysoPC mucosa from 14 day old rats did not change the radioactivity pattern as shown by thin layer chromatography, whereas after incubation with mucosa from 22 or 32 day old animals all the radiolabel was found in free <sup>14</sup>C-fatty acid and in <sup>14</sup>C-phosphatidylcholine. These findings indicate that mucosal PLA<sub>2</sub> activity increases during intestinal maturation and that the mucosa acquires the ability to acylate and deacylate lysoPC when it is 'closed' to macromolecules.</p>}},
  author       = {{Tagesson, C. and Telemo, E. and Ekstrom, G. and Westrom, B.}},
  issn         = {{0017-5749}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{822--828}},
  publisher    = {{BMJ Publishing Group}},
  series       = {{Gut}},
  title        = {{Development of phospholipase A<sub>2</sub> and lysophosphatidylcholine metabolising enzyme activities in the neonatal rat intestine}},
  url          = {{http://dx.doi.org/10.1136/gut.28.7.822}},
  doi          = {{10.1136/gut.28.7.822}},
  volume       = {{28}},
  year         = {{1987}},
}