Preanalytical requirements for flow cytometric evaluation of platelet activation : Choice of anticoagulant
(1999) In Transfusion Medicine 9(2). p.147-154- Abstract
Accurate assessment of in viva or in vitro platelet activation requires optimal preanalytical conditions to prevent artefactual in vitro activation of the platelets. The choice of anticoagulant is one of the critical preanalytical conditions as anticoagulants exert different effects on the activation of platelets ex vivo. We tested the effectiveness of Diatube-H (also known as CTAD; sodium citrate, theophylline, adenosine and dipyridamole) and citrate vacutainer tubes in preventing artefactual activation of platelets and preserving functional reserve. Platelet surface expression of the CD62P (reflecting alpha granule release), CD63 (reflecting lysosomal release) and modulation of normal platelet membrane glycoproteins CD41a and CD42b,... (More)
Accurate assessment of in viva or in vitro platelet activation requires optimal preanalytical conditions to prevent artefactual in vitro activation of the platelets. The choice of anticoagulant is one of the critical preanalytical conditions as anticoagulants exert different effects on the activation of platelets ex vivo. We tested the effectiveness of Diatube-H (also known as CTAD; sodium citrate, theophylline, adenosine and dipyridamole) and citrate vacutainer tubes in preventing artefactual activation of platelets and preserving functional reserve. Platelet surface expression of the CD62P (reflecting alpha granule release), CD63 (reflecting lysosomal release) and modulation of normal platelet membrane glycoproteins CD41a and CD42b, were measured in whole blood and in isolated platelets immediately after collection and at 6, 24 and 48h after venipuncture. Samples taken into Diatube-H showed less spontaneous platelet activation than did those taken into citrate. To measure in vitro platelet functional reserve, thrombin was added as agonist to blood stored for varying periods up to 48h. Although Diatube-H suppressed in vitro platelet activation for up to 4 h, in samples kept for 624 h before thrombin addition, the inhibitory effect was lost and platelets responded fully to agonist activation. Hence, Diatube-H preserved platelets and allowed for measurement of in viva platelet activation as well as thrombin-induced in vitro platelet activation after 624 h, in both whole blood and isolated platelets.
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- author
- Mody, M. ; Lazarus, A. H. ; Semple, J. W. LU and Freedman, J.
- publishing date
- 1999-06-01
- type
- Contribution to journal
- publication status
- published
- keywords
- CTAD, Diatube-H, Flow cytometry, Platelet activation, Platelets, Preanalytical
- in
- Transfusion Medicine
- volume
- 9
- issue
- 2
- pages
- 8 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:10354385
- scopus:0033152960
- ISSN
- 0958-7578
- DOI
- 10.1046/j.1365-3148.1999.00188.x
- language
- English
- LU publication?
- no
- id
- c69d4948-c292-48b9-beff-51b0205b4b59
- date added to LUP
- 2019-12-03 10:29:10
- date last changed
- 2024-06-26 07:33:09
@article{c69d4948-c292-48b9-beff-51b0205b4b59,
abstract = {{<p>Accurate assessment of in viva or in vitro platelet activation requires optimal preanalytical conditions to prevent artefactual in vitro activation of the platelets. The choice of anticoagulant is one of the critical preanalytical conditions as anticoagulants exert different effects on the activation of platelets ex vivo. We tested the effectiveness of Diatube-H (also known as CTAD; sodium citrate, theophylline, adenosine and dipyridamole) and citrate vacutainer tubes in preventing artefactual activation of platelets and preserving functional reserve. Platelet surface expression of the CD62P (reflecting alpha granule release), CD63 (reflecting lysosomal release) and modulation of normal platelet membrane glycoproteins CD41a and CD42b, were measured in whole blood and in isolated platelets immediately after collection and at 6, 24 and 48h after venipuncture. Samples taken into Diatube-H showed less spontaneous platelet activation than did those taken into citrate. To measure in vitro platelet functional reserve, thrombin was added as agonist to blood stored for varying periods up to 48h. Although Diatube-H suppressed in vitro platelet activation for up to 4 h, in samples kept for 624 h before thrombin addition, the inhibitory effect was lost and platelets responded fully to agonist activation. Hence, Diatube-H preserved platelets and allowed for measurement of in viva platelet activation as well as thrombin-induced in vitro platelet activation after 624 h, in both whole blood and isolated platelets.</p>}},
author = {{Mody, M. and Lazarus, A. H. and Semple, J. W. and Freedman, J.}},
issn = {{0958-7578}},
keywords = {{CTAD; Diatube-H; Flow cytometry; Platelet activation; Platelets; Preanalytical}},
language = {{eng}},
month = {{06}},
number = {{2}},
pages = {{147--154}},
publisher = {{Wiley-Blackwell}},
series = {{Transfusion Medicine}},
title = {{Preanalytical requirements for flow cytometric evaluation of platelet activation : Choice of anticoagulant}},
url = {{http://dx.doi.org/10.1046/j.1365-3148.1999.00188.x}},
doi = {{10.1046/j.1365-3148.1999.00188.x}},
volume = {{9}},
year = {{1999}},
}