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Silver-based nanoparticles induce apoptosis in human colon cancer cells mediated through p53

Satapathy, Shakti Ranjan LU ; Mohapatra, Purusottam LU ; Preet, Ranjan ; Das, Dipon ; Sarkar, Biplab ; Choudhuri, Tathagata ; Wyatt, Michael D and Kundu, Chanakya Nath (2013) In Nanomedicine 8(8). p.22-1307
Abstract

AIM: The authors have systematically investigated the anticancer potentiality of silver-based nanoparticles (AgNPs) and the mechanism underlying their biological activity in human colon cancer cells.

MATERIALS & METHODS: Starch-capped AgNPs were synthesized, characterized and their biological activity evaluated through multiple biochemical assays.

RESULTS: AgNPs decreased the growth and viability of HCT116 colon cancer cells. AgNP exposure increased apoptosis, as demonstrated by an increase in 4´,6-diamidino-2-phenylindole-stained apoptotic nuclei, BAX/BCL-XL ratio, cleaved poly(ADP-ribose) polymerase, p53, p21 and caspases 3, 8 and 9, and by a decrease in the levels of AKT and NF-κB. The cell population in the G1 phase... (More)

AIM: The authors have systematically investigated the anticancer potentiality of silver-based nanoparticles (AgNPs) and the mechanism underlying their biological activity in human colon cancer cells.

MATERIALS & METHODS: Starch-capped AgNPs were synthesized, characterized and their biological activity evaluated through multiple biochemical assays.

RESULTS: AgNPs decreased the growth and viability of HCT116 colon cancer cells. AgNP exposure increased apoptosis, as demonstrated by an increase in 4´,6-diamidino-2-phenylindole-stained apoptotic nuclei, BAX/BCL-XL ratio, cleaved poly(ADP-ribose) polymerase, p53, p21 and caspases 3, 8 and 9, and by a decrease in the levels of AKT and NF-κB. The cell population in the G1 phase decreased, and the S-phase population increased after AgNP treatment. AgNPs caused DNA damage and reduced the interaction between p53 and NF-κB. Interestingly, no significant alteration was noted in the levels of p21, BAX/BCL-XL and NF-κB after AgNP treatment in a p53-knockout HCT116 cell line.

CONCLUSION: AgNPs are bona fide anticancer agents that act in a p53-dependent manner. Original submitted 16 March 2012; Revised submitted 25 August 2012; Published online 21 March 2013.

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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Apoptosis/drug effects, Caspase 3/biosynthesis, Colonic Neoplasms/drug therapy, DNA Damage/drug effects, Gene Expression Regulation, Neoplastic/drug effects, HCT116 Cells, Humans, Metal Nanoparticles/administration & dosage, NF-kappa B/biosynthesis, Proto-Oncogene Proteins c-bcl-2/biosynthesis, Silver/administration & dosage, Tumor Suppressor Protein p53/metabolism
in
Nanomedicine
volume
8
issue
8
pages
22 - 1307
publisher
Taylor & Francis
external identifiers
  • scopus:84883753991
  • pmid:23514434
ISSN
1743-5889
DOI
10.2217/nnm.12.176
language
English
LU publication?
no
id
c69eb024-8fcd-4a67-953f-e556eada3752
date added to LUP
2025-01-30 10:31:21
date last changed
2025-05-09 13:43:54
@article{c69eb024-8fcd-4a67-953f-e556eada3752,
  abstract     = {{<p>AIM: The authors have systematically investigated the anticancer potentiality of silver-based nanoparticles (AgNPs) and the mechanism underlying their biological activity in human colon cancer cells.</p><p>MATERIALS &amp; METHODS: Starch-capped AgNPs were synthesized, characterized and their biological activity evaluated through multiple biochemical assays.</p><p>RESULTS: AgNPs decreased the growth and viability of HCT116 colon cancer cells. AgNP exposure increased apoptosis, as demonstrated by an increase in 4´,6-diamidino-2-phenylindole-stained apoptotic nuclei, BAX/BCL-XL ratio, cleaved poly(ADP-ribose) polymerase, p53, p21 and caspases 3, 8 and 9, and by a decrease in the levels of AKT and NF-κB. The cell population in the G1 phase decreased, and the S-phase population increased after AgNP treatment. AgNPs caused DNA damage and reduced the interaction between p53 and NF-κB. Interestingly, no significant alteration was noted in the levels of p21, BAX/BCL-XL and NF-κB after AgNP treatment in a p53-knockout HCT116 cell line.</p><p>CONCLUSION: AgNPs are bona fide anticancer agents that act in a p53-dependent manner. Original submitted 16 March 2012; Revised submitted 25 August 2012; Published online 21 March 2013.</p>}},
  author       = {{Satapathy, Shakti Ranjan and Mohapatra, Purusottam and Preet, Ranjan and Das, Dipon and Sarkar, Biplab and Choudhuri, Tathagata and Wyatt, Michael D and Kundu, Chanakya Nath}},
  issn         = {{1743-5889}},
  keywords     = {{Apoptosis/drug effects; Caspase 3/biosynthesis; Colonic Neoplasms/drug therapy; DNA Damage/drug effects; Gene Expression Regulation, Neoplastic/drug effects; HCT116 Cells; Humans; Metal Nanoparticles/administration & dosage; NF-kappa B/biosynthesis; Proto-Oncogene Proteins c-bcl-2/biosynthesis; Silver/administration & dosage; Tumor Suppressor Protein p53/metabolism}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{22--1307}},
  publisher    = {{Taylor & Francis}},
  series       = {{Nanomedicine}},
  title        = {{Silver-based nanoparticles induce apoptosis in human colon cancer cells mediated through p53}},
  url          = {{http://dx.doi.org/10.2217/nnm.12.176}},
  doi          = {{10.2217/nnm.12.176}},
  volume       = {{8}},
  year         = {{2013}},
}