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The tympanic membrane and middle ear mucosa during non-typeable Haemophilus influenzae and Haemophilus influenzae type b acute otitis media : A study in the rat

Magnuson, Karin LU orcid ; Hermansson, Ann LU ; Melhus, Åsa LU and Hellström, Sten (1997) In Acta Oto-Laryngologica 117(3). p.396-405
Abstract

Non-typeable Haemophilus influenzae (NTHi) and encapsulated Haemophilus influenzae type b (Hib) were inoculated into the middle ears of Sprague- Dawley rats. Tympanic membrane (TM) status was assessed otomicroscopically and specimens from various middle ear areas were prepared for light microscopy at various times during the acute phase and up to 6 months after inoculation. Irrespective of bacteria strain, acute otitis media (AOM) was present in all ears 4 days after inoculation. The Hib-infected ears showed initially a severe course of AOM, but all were otomicroscopically resolved by day 12, at which time a few NTHi-inoculated ears still exhibited middle ear effusion. The TMs infected with Hib had normalized without scar formation,... (More)

Non-typeable Haemophilus influenzae (NTHi) and encapsulated Haemophilus influenzae type b (Hib) were inoculated into the middle ears of Sprague- Dawley rats. Tympanic membrane (TM) status was assessed otomicroscopically and specimens from various middle ear areas were prepared for light microscopy at various times during the acute phase and up to 6 months after inoculation. Irrespective of bacteria strain, acute otitis media (AOM) was present in all ears 4 days after inoculation. The Hib-infected ears showed initially a severe course of AOM, but all were otomicroscopically resolved by day 12, at which time a few NTHi-inoculated ears still exhibited middle ear effusion. The TMs infected with Hib had normalized without scar formation, whereas NTHi induced a persistent thickening of the TMs in half of all cases. The middle ear mucosa of NTHi-infected ears initially showed vigorous activity among the goblet cells, but the mucosa normalized after the acute phase Hib, by contrast induced prominent changes in the middle ear mucosa. Initially, no goblet cell granules or ciliated cells could be observed in the mucosa. Later on, the epithelium contained large, active goblet cells. Glands appeared beneath the mucosa which persisted as streaks of epithelial cells throughout the study period. The findings show that NTHi and Hib both induce AOM but with differing clinical courses, and affect different targets in the middle ear.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
goblet cells, light microscopy, middle ear effusion, otomicroscopy, scar formation
in
Acta Oto-Laryngologica
volume
117
issue
3
pages
10 pages
publisher
Taylor & Francis
external identifiers
  • pmid:9199526
  • scopus:0031005923
ISSN
0001-6489
DOI
10.3109/00016489709113412
language
English
LU publication?
yes
id
c6a57b4a-dbd6-4c66-89ea-c017992a16f9
date added to LUP
2019-05-08 12:53:33
date last changed
2024-04-02 01:38:35
@article{c6a57b4a-dbd6-4c66-89ea-c017992a16f9,
  abstract     = {{<p>Non-typeable Haemophilus influenzae (NTHi) and encapsulated Haemophilus influenzae type b (Hib) were inoculated into the middle ears of Sprague- Dawley rats. Tympanic membrane (TM) status was assessed otomicroscopically and specimens from various middle ear areas were prepared for light microscopy at various times during the acute phase and up to 6 months after inoculation. Irrespective of bacteria strain, acute otitis media (AOM) was present in all ears 4 days after inoculation. The Hib-infected ears showed initially a severe course of AOM, but all were otomicroscopically resolved by day 12, at which time a few NTHi-inoculated ears still exhibited middle ear effusion. The TMs infected with Hib had normalized without scar formation, whereas NTHi induced a persistent thickening of the TMs in half of all cases. The middle ear mucosa of NTHi-infected ears initially showed vigorous activity among the goblet cells, but the mucosa normalized after the acute phase Hib, by contrast induced prominent changes in the middle ear mucosa. Initially, no goblet cell granules or ciliated cells could be observed in the mucosa. Later on, the epithelium contained large, active goblet cells. Glands appeared beneath the mucosa which persisted as streaks of epithelial cells throughout the study period. The findings show that NTHi and Hib both induce AOM but with differing clinical courses, and affect different targets in the middle ear.</p>}},
  author       = {{Magnuson, Karin and Hermansson, Ann and Melhus, Åsa and Hellström, Sten}},
  issn         = {{0001-6489}},
  keywords     = {{goblet cells; light microscopy; middle ear effusion; otomicroscopy; scar formation}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{3}},
  pages        = {{396--405}},
  publisher    = {{Taylor & Francis}},
  series       = {{Acta Oto-Laryngologica}},
  title        = {{The tympanic membrane and middle ear mucosa during non-typeable Haemophilus influenzae and Haemophilus influenzae type b acute otitis media : A study in the rat}},
  url          = {{http://dx.doi.org/10.3109/00016489709113412}},
  doi          = {{10.3109/00016489709113412}},
  volume       = {{117}},
  year         = {{1997}},
}