Design of a potentially prebiotic and responsive encapsulation material for probiotic bacteria based on chitosan and sulfated β-glucan
(2017) In Journal of Colloid and Interface Science 487. p.97-106- Abstract
Hypothesis Chitosan and sulfated oat β-glucan are materials suitable to create a prebiotic coating for targeted delivery to gastrointestinal system, using the layer by layer technology. Experiment Quartz crystal microbalance with dissipation (QCM-D), spectroscopic ellipsometry (SE) and atomic force microscopy (AFM) were used to assess the multilayer formation capacity and characterize the resulting coatings in terms of morphology and material properties such as structure and rigidity. The coating of colloidal materials was proven, specifically on L. acidophilus bacteria as measured by changes in the bacterial suspension zeta potential. Viability of coated cells was shown using plate counting method. The coatings on solid surfaces were... (More)
Hypothesis Chitosan and sulfated oat β-glucan are materials suitable to create a prebiotic coating for targeted delivery to gastrointestinal system, using the layer by layer technology. Experiment Quartz crystal microbalance with dissipation (QCM-D), spectroscopic ellipsometry (SE) and atomic force microscopy (AFM) were used to assess the multilayer formation capacity and characterize the resulting coatings in terms of morphology and material properties such as structure and rigidity. The coating of colloidal materials was proven, specifically on L. acidophilus bacteria as measured by changes in the bacterial suspension zeta potential. Viability of coated cells was shown using plate counting method. The coatings on solid surfaces were examined after exposure to mimics of gastrointestinal fluids and a commercially available β-glucanase. Findings Successful build-up of multilayers was confirmed with QCM-D and SE. Zeta potential values proved the coating of cells. There was 2 log CFU/mL decrease after coating cells with four alternating layers of chitosan and sulfated β-glucan when compared to viability of uncoated cells. The coatings were partially degraded after exposure to simulated intestinal fluid and restructured as a result of β-glucanase treatment, mimicking enzymes present in the microflora of the human gut, but seemed to resist acidic gastric conditions. Therefore, coatings of chitosan and sulfated β-glucan can potentially be exploited as carriers for probiotics and delicate nutraceuticals.
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- author
- Yucel Falco, Cigdem ; Sotres, Javier ; Rascón, Ana LU ; Risbo, Jens and Cárdenas, Marité LU
- organization
- publishing date
- 2017-02-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Chitosan, Layer by layer, Prebiotics, Probiotics, Sulfated oat β-glucan
- in
- Journal of Colloid and Interface Science
- volume
- 487
- pages
- 10 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:27756004
- wos:000388550600012
- scopus:84992490693
- ISSN
- 0021-9797
- DOI
- 10.1016/j.jcis.2016.10.019
- language
- English
- LU publication?
- yes
- id
- c6b4c6b7-fe9b-406d-9439-f7b16b729075
- date added to LUP
- 2017-02-03 12:16:31
- date last changed
- 2024-06-24 13:44:59
@article{c6b4c6b7-fe9b-406d-9439-f7b16b729075,
abstract = {{<p>Hypothesis Chitosan and sulfated oat β-glucan are materials suitable to create a prebiotic coating for targeted delivery to gastrointestinal system, using the layer by layer technology. Experiment Quartz crystal microbalance with dissipation (QCM-D), spectroscopic ellipsometry (SE) and atomic force microscopy (AFM) were used to assess the multilayer formation capacity and characterize the resulting coatings in terms of morphology and material properties such as structure and rigidity. The coating of colloidal materials was proven, specifically on L. acidophilus bacteria as measured by changes in the bacterial suspension zeta potential. Viability of coated cells was shown using plate counting method. The coatings on solid surfaces were examined after exposure to mimics of gastrointestinal fluids and a commercially available β-glucanase. Findings Successful build-up of multilayers was confirmed with QCM-D and SE. Zeta potential values proved the coating of cells. There was 2 log CFU/mL decrease after coating cells with four alternating layers of chitosan and sulfated β-glucan when compared to viability of uncoated cells. The coatings were partially degraded after exposure to simulated intestinal fluid and restructured as a result of β-glucanase treatment, mimicking enzymes present in the microflora of the human gut, but seemed to resist acidic gastric conditions. Therefore, coatings of chitosan and sulfated β-glucan can potentially be exploited as carriers for probiotics and delicate nutraceuticals.</p>}},
author = {{Yucel Falco, Cigdem and Sotres, Javier and Rascón, Ana and Risbo, Jens and Cárdenas, Marité}},
issn = {{0021-9797}},
keywords = {{Chitosan; Layer by layer; Prebiotics; Probiotics; Sulfated oat β-glucan}},
language = {{eng}},
month = {{02}},
pages = {{97--106}},
publisher = {{Elsevier}},
series = {{Journal of Colloid and Interface Science}},
title = {{Design of a potentially prebiotic and responsive encapsulation material for probiotic bacteria based on chitosan and sulfated β-glucan}},
url = {{http://dx.doi.org/10.1016/j.jcis.2016.10.019}},
doi = {{10.1016/j.jcis.2016.10.019}},
volume = {{487}},
year = {{2017}},
}