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Design of a potentially prebiotic and responsive encapsulation material for probiotic bacteria based on chitosan and sulfated β-glucan

Yucel Falco, Cigdem; Sotres, Javier; Rascón, Ana LU ; Risbo, Jens and Cárdenas, Marité LU (2017) In Journal of Colloid and Interface Science 487. p.97-106
Abstract

Hypothesis Chitosan and sulfated oat β-glucan are materials suitable to create a prebiotic coating for targeted delivery to gastrointestinal system, using the layer by layer technology. Experiment Quartz crystal microbalance with dissipation (QCM-D), spectroscopic ellipsometry (SE) and atomic force microscopy (AFM) were used to assess the multilayer formation capacity and characterize the resulting coatings in terms of morphology and material properties such as structure and rigidity. The coating of colloidal materials was proven, specifically on L. acidophilus bacteria as measured by changes in the bacterial suspension zeta potential. Viability of coated cells was shown using plate counting method. The coatings on solid surfaces were... (More)

Hypothesis Chitosan and sulfated oat β-glucan are materials suitable to create a prebiotic coating for targeted delivery to gastrointestinal system, using the layer by layer technology. Experiment Quartz crystal microbalance with dissipation (QCM-D), spectroscopic ellipsometry (SE) and atomic force microscopy (AFM) were used to assess the multilayer formation capacity and characterize the resulting coatings in terms of morphology and material properties such as structure and rigidity. The coating of colloidal materials was proven, specifically on L. acidophilus bacteria as measured by changes in the bacterial suspension zeta potential. Viability of coated cells was shown using plate counting method. The coatings on solid surfaces were examined after exposure to mimics of gastrointestinal fluids and a commercially available β-glucanase. Findings Successful build-up of multilayers was confirmed with QCM-D and SE. Zeta potential values proved the coating of cells. There was 2 log CFU/mL decrease after coating cells with four alternating layers of chitosan and sulfated β-glucan when compared to viability of uncoated cells. The coatings were partially degraded after exposure to simulated intestinal fluid and restructured as a result of β-glucanase treatment, mimicking enzymes present in the microflora of the human gut, but seemed to resist acidic gastric conditions. Therefore, coatings of chitosan and sulfated β-glucan can potentially be exploited as carriers for probiotics and delicate nutraceuticals.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Chitosan, Layer by layer, Prebiotics, Probiotics, Sulfated oat β-glucan
in
Journal of Colloid and Interface Science
volume
487
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:84992490693
  • wos:000388550600012
ISSN
0021-9797
DOI
10.1016/j.jcis.2016.10.019
language
English
LU publication?
yes
id
c6b4c6b7-fe9b-406d-9439-f7b16b729075
date added to LUP
2017-02-03 12:16:31
date last changed
2018-01-07 11:48:01
@article{c6b4c6b7-fe9b-406d-9439-f7b16b729075,
  abstract     = {<p>Hypothesis Chitosan and sulfated oat β-glucan are materials suitable to create a prebiotic coating for targeted delivery to gastrointestinal system, using the layer by layer technology. Experiment Quartz crystal microbalance with dissipation (QCM-D), spectroscopic ellipsometry (SE) and atomic force microscopy (AFM) were used to assess the multilayer formation capacity and characterize the resulting coatings in terms of morphology and material properties such as structure and rigidity. The coating of colloidal materials was proven, specifically on L. acidophilus bacteria as measured by changes in the bacterial suspension zeta potential. Viability of coated cells was shown using plate counting method. The coatings on solid surfaces were examined after exposure to mimics of gastrointestinal fluids and a commercially available β-glucanase. Findings Successful build-up of multilayers was confirmed with QCM-D and SE. Zeta potential values proved the coating of cells. There was 2 log CFU/mL decrease after coating cells with four alternating layers of chitosan and sulfated β-glucan when compared to viability of uncoated cells. The coatings were partially degraded after exposure to simulated intestinal fluid and restructured as a result of β-glucanase treatment, mimicking enzymes present in the microflora of the human gut, but seemed to resist acidic gastric conditions. Therefore, coatings of chitosan and sulfated β-glucan can potentially be exploited as carriers for probiotics and delicate nutraceuticals.</p>},
  author       = {Yucel Falco, Cigdem and Sotres, Javier and Rascón, Ana and Risbo, Jens and Cárdenas, Marité},
  issn         = {0021-9797},
  keyword      = {Chitosan,Layer by layer,Prebiotics,Probiotics,Sulfated oat β-glucan},
  language     = {eng},
  month        = {02},
  pages        = {97--106},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {Design of a potentially prebiotic and responsive encapsulation material for probiotic bacteria based on chitosan and sulfated β-glucan},
  url          = {http://dx.doi.org/10.1016/j.jcis.2016.10.019},
  volume       = {487},
  year         = {2017},
}