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Cambios geneticos en cepas de poliovirus tipo 2 aisladas de pacientes con poliomielitis paralitica asociada a la vacuna

Sosa-Díaz, R Y; Más-Lago, P; Valdés-Ramírez, O and Sarmiento-Pérez, L LU (2003) In Revista de Neurologia 36(9). p.6-812
Abstract

INTRODUCTION: Poliomyelitis is currently a rare disease in developed countries, where only vaccinal strains seem to be in circulation, which replace wild poliovirus. Nevertheless, it is still a serious disease for children in underdeveloped countries of Asia and Africa.

PATIENTS AND METHODS: We analysed nine strains of poliovirus type 2 isolated from the faecal matter of patients with vaccine associated paralytic poliomyelitis (VAPP), from the beginning of anti polio vaccination campaigns in our country. These strains were submitted to sequencing of a fragment of 114 base pairs from the 5 NTR (non traductional region), where one of the main determinants of attenuation/reversion to the neurovirulence of poliovirus lies in the... (More)

INTRODUCTION: Poliomyelitis is currently a rare disease in developed countries, where only vaccinal strains seem to be in circulation, which replace wild poliovirus. Nevertheless, it is still a serious disease for children in underdeveloped countries of Asia and Africa.

PATIENTS AND METHODS: We analysed nine strains of poliovirus type 2 isolated from the faecal matter of patients with vaccine associated paralytic poliomyelitis (VAPP), from the beginning of anti polio vaccination campaigns in our country. These strains were submitted to sequencing of a fragment of 114 base pairs from the 5 NTR (non traductional region), where one of the main determinants of attenuation/reversion to the neurovirulence of poliovirus lies in the position of nucleotide 481.

RESULTS: In this position it was observed how guanine had been replaced by adenine in all the strains that were sequenced, so that it coincided with the homologous sequence of the wild strain, as well as with that of strains obtained from healthy children immunised with the live vaccine. This presupposes that other changes must occur or that other factors must be involved for VAPP to occur or not, and we therefore suggest the sequencing of other regions of the genome in search of other possible differential changes in nucleotides.

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author
alternative title
Genetic changes in strains of poliovirus type 2 isolated from patients with vaccine-associated paralytic poliomyelitis
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Child, Child, Preschool, Humans, Point Mutation/genetics, Poliomyelitis, Bulbar/prevention & control, Poliovirus/genetics, Poliovirus Vaccine, Oral/administration & dosage, Reverse Transcriptase Polymerase Chain Reaction, Vaccines, Attenuated/administration & dosage
in
Revista de Neurologia
volume
36
issue
9
pages
5 pages
publisher
Revista de Neurologia
external identifiers
  • scopus:0038650673
ISSN
0210-0010
DOI
10.33588/rn.3609.2002309
language
Spanish
LU publication?
no
id
c6da4ea3-5039-41f2-ae19-f8e8439731df
date added to LUP
2019-07-08 15:05:27
date last changed
2019-08-14 09:49:12
@article{c6da4ea3-5039-41f2-ae19-f8e8439731df,
  abstract     = {<p>INTRODUCTION: Poliomyelitis is currently a rare disease in developed countries, where only vaccinal strains seem to be in circulation, which replace wild poliovirus. Nevertheless, it is still a serious disease for children in underdeveloped countries of Asia and Africa.</p><p>PATIENTS AND METHODS: We analysed nine strains of poliovirus type 2 isolated from the faecal matter of patients with vaccine associated paralytic poliomyelitis (VAPP), from the beginning of anti polio vaccination campaigns in our country. These strains were submitted to sequencing of a fragment of 114 base pairs from the 5 NTR (non traductional region), where one of the main determinants of attenuation/reversion to the neurovirulence of poliovirus lies in the position of nucleotide 481.</p><p>RESULTS: In this position it was observed how guanine had been replaced by adenine in all the strains that were sequenced, so that it coincided with the homologous sequence of the wild strain, as well as with that of strains obtained from healthy children immunised with the live vaccine. This presupposes that other changes must occur or that other factors must be involved for VAPP to occur or not, and we therefore suggest the sequencing of other regions of the genome in search of other possible differential changes in nucleotides.</p>},
  author       = {Sosa-Díaz, R Y and Más-Lago, P and Valdés-Ramírez, O and Sarmiento-Pérez, L},
  issn         = {0210-0010},
  keyword      = {Child,Child, Preschool,Humans,Point Mutation/genetics,Poliomyelitis, Bulbar/prevention & control,Poliovirus/genetics,Poliovirus Vaccine, Oral/administration & dosage,Reverse Transcriptase Polymerase Chain Reaction,Vaccines, Attenuated/administration & dosage},
  language     = {spa},
  month        = {04},
  number       = {9},
  pages        = {6--812},
  publisher    = {Revista de Neurologia},
  series       = {Revista de Neurologia},
  title        = {Cambios geneticos en cepas de poliovirus tipo 2 aisladas de pacientes con poliomielitis paralitica asociada a la vacuna},
  url          = {http://dx.doi.org/10.33588/rn.3609.2002309},
  volume       = {36},
  year         = {2003},
}