Adventitial Stromal Cells Define Group 2 Innate Lymphoid Cell Tissue Niches
(2019) In Immunity 50(3). p.6-722- Abstract
Type 2 lymphocytes promote both physiologic tissue remodeling and allergic pathology, yet their physical tissue niches are poorly described. Here, we used quantitative imaging to define the tissue niches of group 2 innate lymphoid cells (ILC2s), which are critical instigators of type 2 immunity. We identified a dominant adventitial niche around lung bronchi and larger vessels in multiple tissues, where ILC2s localized with subsets of dendritic and regulatory T cells. However, ILC2s were most intimately associated with adventitial stromal cells (ASCs), a mesenchymal fibroblast-like subset that expresses interleukin-33 (IL-33) and thymic stromal lymphopoietin (TSLP). In vitro, ASCs produced TSLP that supported ILC2 accumulation and... (More)
Type 2 lymphocytes promote both physiologic tissue remodeling and allergic pathology, yet their physical tissue niches are poorly described. Here, we used quantitative imaging to define the tissue niches of group 2 innate lymphoid cells (ILC2s), which are critical instigators of type 2 immunity. We identified a dominant adventitial niche around lung bronchi and larger vessels in multiple tissues, where ILC2s localized with subsets of dendritic and regulatory T cells. However, ILC2s were most intimately associated with adventitial stromal cells (ASCs), a mesenchymal fibroblast-like subset that expresses interleukin-33 (IL-33) and thymic stromal lymphopoietin (TSLP). In vitro, ASCs produced TSLP that supported ILC2 accumulation and activation. ILC2s and IL-13 drove reciprocal ASC expansion and IL-33 expression. During helminth infection, ASC depletion impaired lung ILC2 and Th2 cell accumulation and function, which are in part dependent on ASC-derived IL-33. These data indicate that adventitial niches are conserved sites where ASCs regulate type 2 lymphocyte expansion and function.
(Less)
- author
- publishing date
- 2019-03-19
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Bronchi/immunology, Cytokines/immunology, Immunity, Innate/immunology, Interleukin-13/immunology, Interleukin-33/immunology, Lymphocytes/immunology, Mice, Stromal Cells/immunology, T-Lymphocytes, Regulatory/immunology, Th2 Cells/immunology, Thymic Stromal Lymphopoietin
- in
- Immunity
- volume
- 50
- issue
- 3
- pages
- 6 - 722
- publisher
- Cell Press
- external identifiers
-
- scopus:85062867999
- pmid:30824323
- ISSN
- 1074-7613
- DOI
- 10.1016/j.immuni.2019.02.002
- language
- English
- LU publication?
- no
- additional info
- Copyright © 2019 Elsevier Inc. All rights reserved.
- id
- c6fa7388-b9ac-4a68-8399-8af49f6fa5a7
- date added to LUP
- 2024-05-06 00:00:56
- date last changed
- 2024-05-20 06:34:40
@article{c6fa7388-b9ac-4a68-8399-8af49f6fa5a7, abstract = {{<p>Type 2 lymphocytes promote both physiologic tissue remodeling and allergic pathology, yet their physical tissue niches are poorly described. Here, we used quantitative imaging to define the tissue niches of group 2 innate lymphoid cells (ILC2s), which are critical instigators of type 2 immunity. We identified a dominant adventitial niche around lung bronchi and larger vessels in multiple tissues, where ILC2s localized with subsets of dendritic and regulatory T cells. However, ILC2s were most intimately associated with adventitial stromal cells (ASCs), a mesenchymal fibroblast-like subset that expresses interleukin-33 (IL-33) and thymic stromal lymphopoietin (TSLP). In vitro, ASCs produced TSLP that supported ILC2 accumulation and activation. ILC2s and IL-13 drove reciprocal ASC expansion and IL-33 expression. During helminth infection, ASC depletion impaired lung ILC2 and Th2 cell accumulation and function, which are in part dependent on ASC-derived IL-33. These data indicate that adventitial niches are conserved sites where ASCs regulate type 2 lymphocyte expansion and function.</p>}}, author = {{Dahlgren, Madelene W and Jones, Stephen W and Cautivo, Kelly M and Dubinin, Alexandra and Ortiz-Carpena, Jorge F and Farhat, Sepideh and Yu, Kevin S and Lee, Katharine and Wang, Chaoqun and Molofsky, Anna V and Tward, Aaron D and Krummel, Matthew F and Peng, Tien and Molofsky, Ari B}}, issn = {{1074-7613}}, keywords = {{Animals; Bronchi/immunology; Cytokines/immunology; Immunity, Innate/immunology; Interleukin-13/immunology; Interleukin-33/immunology; Lymphocytes/immunology; Mice; Stromal Cells/immunology; T-Lymphocytes, Regulatory/immunology; Th2 Cells/immunology; Thymic Stromal Lymphopoietin}}, language = {{eng}}, month = {{03}}, number = {{3}}, pages = {{6--722}}, publisher = {{Cell Press}}, series = {{Immunity}}, title = {{Adventitial Stromal Cells Define Group 2 Innate Lymphoid Cell Tissue Niches}}, url = {{http://dx.doi.org/10.1016/j.immuni.2019.02.002}}, doi = {{10.1016/j.immuni.2019.02.002}}, volume = {{50}}, year = {{2019}}, }