Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Adventitial Stromal Cells Define Group 2 Innate Lymphoid Cell Tissue Niches

Dahlgren, Madelene W LU orcid ; Jones, Stephen W ; Cautivo, Kelly M ; Dubinin, Alexandra ; Ortiz-Carpena, Jorge F ; Farhat, Sepideh ; Yu, Kevin S ; Lee, Katharine ; Wang, Chaoqun and Molofsky, Anna V , et al. (2019) In Immunity 50(3). p.6-722
Abstract

Type 2 lymphocytes promote both physiologic tissue remodeling and allergic pathology, yet their physical tissue niches are poorly described. Here, we used quantitative imaging to define the tissue niches of group 2 innate lymphoid cells (ILC2s), which are critical instigators of type 2 immunity. We identified a dominant adventitial niche around lung bronchi and larger vessels in multiple tissues, where ILC2s localized with subsets of dendritic and regulatory T cells. However, ILC2s were most intimately associated with adventitial stromal cells (ASCs), a mesenchymal fibroblast-like subset that expresses interleukin-33 (IL-33) and thymic stromal lymphopoietin (TSLP). In vitro, ASCs produced TSLP that supported ILC2 accumulation and... (More)

Type 2 lymphocytes promote both physiologic tissue remodeling and allergic pathology, yet their physical tissue niches are poorly described. Here, we used quantitative imaging to define the tissue niches of group 2 innate lymphoid cells (ILC2s), which are critical instigators of type 2 immunity. We identified a dominant adventitial niche around lung bronchi and larger vessels in multiple tissues, where ILC2s localized with subsets of dendritic and regulatory T cells. However, ILC2s were most intimately associated with adventitial stromal cells (ASCs), a mesenchymal fibroblast-like subset that expresses interleukin-33 (IL-33) and thymic stromal lymphopoietin (TSLP). In vitro, ASCs produced TSLP that supported ILC2 accumulation and activation. ILC2s and IL-13 drove reciprocal ASC expansion and IL-33 expression. During helminth infection, ASC depletion impaired lung ILC2 and Th2 cell accumulation and function, which are in part dependent on ASC-derived IL-33. These data indicate that adventitial niches are conserved sites where ASCs regulate type 2 lymphocyte expansion and function.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; ; and (Less)
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Bronchi/immunology, Cytokines/immunology, Immunity, Innate/immunology, Interleukin-13/immunology, Interleukin-33/immunology, Lymphocytes/immunology, Mice, Stromal Cells/immunology, T-Lymphocytes, Regulatory/immunology, Th2 Cells/immunology, Thymic Stromal Lymphopoietin
in
Immunity
volume
50
issue
3
pages
6 - 722
publisher
Cell Press
external identifiers
  • scopus:85062867999
  • pmid:30824323
ISSN
1074-7613
DOI
10.1016/j.immuni.2019.02.002
language
English
LU publication?
no
additional info
Copyright © 2019 Elsevier Inc. All rights reserved.
id
c6fa7388-b9ac-4a68-8399-8af49f6fa5a7
date added to LUP
2024-05-06 00:00:56
date last changed
2024-05-20 06:34:40
@article{c6fa7388-b9ac-4a68-8399-8af49f6fa5a7,
  abstract     = {{<p>Type 2 lymphocytes promote both physiologic tissue remodeling and allergic pathology, yet their physical tissue niches are poorly described. Here, we used quantitative imaging to define the tissue niches of group 2 innate lymphoid cells (ILC2s), which are critical instigators of type 2 immunity. We identified a dominant adventitial niche around lung bronchi and larger vessels in multiple tissues, where ILC2s localized with subsets of dendritic and regulatory T cells. However, ILC2s were most intimately associated with adventitial stromal cells (ASCs), a mesenchymal fibroblast-like subset that expresses interleukin-33 (IL-33) and thymic stromal lymphopoietin (TSLP). In vitro, ASCs produced TSLP that supported ILC2 accumulation and activation. ILC2s and IL-13 drove reciprocal ASC expansion and IL-33 expression. During helminth infection, ASC depletion impaired lung ILC2 and Th2 cell accumulation and function, which are in part dependent on ASC-derived IL-33. These data indicate that adventitial niches are conserved sites where ASCs regulate type 2 lymphocyte expansion and function.</p>}},
  author       = {{Dahlgren, Madelene W and Jones, Stephen W and Cautivo, Kelly M and Dubinin, Alexandra and Ortiz-Carpena, Jorge F and Farhat, Sepideh and Yu, Kevin S and Lee, Katharine and Wang, Chaoqun and Molofsky, Anna V and Tward, Aaron D and Krummel, Matthew F and Peng, Tien and Molofsky, Ari B}},
  issn         = {{1074-7613}},
  keywords     = {{Animals; Bronchi/immunology; Cytokines/immunology; Immunity, Innate/immunology; Interleukin-13/immunology; Interleukin-33/immunology; Lymphocytes/immunology; Mice; Stromal Cells/immunology; T-Lymphocytes, Regulatory/immunology; Th2 Cells/immunology; Thymic Stromal Lymphopoietin}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{3}},
  pages        = {{6--722}},
  publisher    = {{Cell Press}},
  series       = {{Immunity}},
  title        = {{Adventitial Stromal Cells Define Group 2 Innate Lymphoid Cell Tissue Niches}},
  url          = {{http://dx.doi.org/10.1016/j.immuni.2019.02.002}},
  doi          = {{10.1016/j.immuni.2019.02.002}},
  volume       = {{50}},
  year         = {{2019}},
}