The transcription factor KLF11 can induce gamma-globin gene expression in the setting of in vivo adult erythropoiesis
(2007) In Journal of Cellular Biochemistry 100(4). p.55-1045- Abstract
- Previous studies in a fetal erythroid cell line demonstrated that the transcription factor, Kruppel-like factor 11 (KLF11), could specifically induce transcription from a gamma-globin gene promoter, and that this induction was mediated through a specific canonical CACCC cis-DNA binding motif. We report here that ectopic expression of KLF11 can also induce fetal gamma-globin gene expression in the setting of adult erythropoiesis both in vitro and in vivo. Studies in an adult-stage murine erythroleukemia (MEL) cell line demonstrated that retrovirus vector-mediated transduction of KLF11 could increase both the amount of expression from a basally active, but not from a overtly silenced, recombinant gamma-globin transgene, as well as the... (More)
- Previous studies in a fetal erythroid cell line demonstrated that the transcription factor, Kruppel-like factor 11 (KLF11), could specifically induce transcription from a gamma-globin gene promoter, and that this induction was mediated through a specific canonical CACCC cis-DNA binding motif. We report here that ectopic expression of KLF11 can also induce fetal gamma-globin gene expression in the setting of adult erythropoiesis both in vitro and in vivo. Studies in an adult-stage murine erythroleukemia (MEL) cell line demonstrated that retrovirus vector-mediated transduction of KLF11 could increase both the amount of expression from a basally active, but not from a overtly silenced, recombinant gamma-globin transgene, as well as the frequency of cells expressing this transgene. A similar pattern of gamma-globin gene induction was also observed both in vitro and in vivo following KLF11 transduction of bone marrow from mice containing a basally active gamma-globin transgene. These studies provide the first evidence that ectopic expression of a transcription factor can induce gamma-globin gene expression in vivo during adult erythropoiesis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4091043
- author
- Emery, D. W. ; Gavriilidis, Georgios LU ; Asano, H. and Stamatoyannopoulos, G.
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Kruppel-Like Transcription Factors/genetics/metabolism/*physiology, Humans, Green Fluorescent Proteins/genetics/metabolism, Globins/*genetics/metabolism, *Gene Expression, Flow Cytometry, Erythropoiesis/*genetics, Erythroid Precursor Cells/cytology/metabolism, Cell Line, Animals, Bone Marrow Transplantation, Mice, Transgenic, NIH 3T3 Cells, Retroviridae/genetics, Time Factors, Transfection
- in
- Journal of Cellular Biochemistry
- volume
- 100
- issue
- 4
- pages
- 55 - 1045
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:33847039607
- ISSN
- 0730-2312
- DOI
- 10.1002/jcb.21093
- language
- English
- LU publication?
- no
- id
- c7058b32-66c5-40f3-a7a3-b9f54a7c05aa (old id 4091043)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/17131378
- http://www.ncbi.nlm.nih.gov/pubmed/17131378
- date added to LUP
- 2016-04-04 08:32:07
- date last changed
- 2022-03-23 02:31:27
@article{c7058b32-66c5-40f3-a7a3-b9f54a7c05aa,
abstract = {{Previous studies in a fetal erythroid cell line demonstrated that the transcription factor, Kruppel-like factor 11 (KLF11), could specifically induce transcription from a gamma-globin gene promoter, and that this induction was mediated through a specific canonical CACCC cis-DNA binding motif. We report here that ectopic expression of KLF11 can also induce fetal gamma-globin gene expression in the setting of adult erythropoiesis both in vitro and in vivo. Studies in an adult-stage murine erythroleukemia (MEL) cell line demonstrated that retrovirus vector-mediated transduction of KLF11 could increase both the amount of expression from a basally active, but not from a overtly silenced, recombinant gamma-globin transgene, as well as the frequency of cells expressing this transgene. A similar pattern of gamma-globin gene induction was also observed both in vitro and in vivo following KLF11 transduction of bone marrow from mice containing a basally active gamma-globin transgene. These studies provide the first evidence that ectopic expression of a transcription factor can induce gamma-globin gene expression in vivo during adult erythropoiesis.}},
author = {{Emery, D. W. and Gavriilidis, Georgios and Asano, H. and Stamatoyannopoulos, G.}},
issn = {{0730-2312}},
keywords = {{Kruppel-Like Transcription Factors/genetics/metabolism/*physiology; Humans; Green Fluorescent Proteins/genetics/metabolism; Globins/*genetics/metabolism; *Gene Expression; Flow Cytometry; Erythropoiesis/*genetics; Erythroid Precursor Cells/cytology/metabolism; Cell Line; Animals; Bone Marrow Transplantation; Mice; Transgenic; NIH 3T3 Cells; Retroviridae/genetics; Time Factors; Transfection}},
language = {{eng}},
number = {{4}},
pages = {{55--1045}},
publisher = {{Wiley-Blackwell}},
series = {{Journal of Cellular Biochemistry}},
title = {{The transcription factor KLF11 can induce gamma-globin gene expression in the setting of in vivo adult erythropoiesis}},
url = {{http://dx.doi.org/10.1002/jcb.21093}},
doi = {{10.1002/jcb.21093}},
volume = {{100}},
year = {{2007}},
}