The human milk microbiome aligns with lactation stage and not birth mode
Lyons, Katriona E. ; Shea, Carol Anne O.’ ; Grimaud, Ghjuvan LU
; Ryan, C. Anthony
; Dempsey, Eugene
; Kelly, Alan L.
; Ross, R. Paul
and Stanton, Catherine
(2022)
In Scientific Reports
12(1).
- Abstract
We analysed the human milk microbiome in a cohort of 80 lactating women and followed the dynamics in taxa over the course of lactation from birth to 6 months. Two hundred and thirty one milk samples were collected from full-term lactating women at 1, 4, 8 and 24 weeks following birth and analysed for microbiota composition using 16S rRNA sequencing. A significant decrease in milk microbiota diversity was observed throughout the first 6 months of lactation, with the greatest difference seen between week 8 and week 24. Nine genera predominated in milk over lactation from week 1 to week 24, comprising of Staphylococcus, Streptococcus, Pseudomonas, Acinetobacter, Bifidobacterium, Mesorhizobium, Brevundimonas, Flavobacterium, and... (More)
We analysed the human milk microbiome in a cohort of 80 lactating women and followed the dynamics in taxa over the course of lactation from birth to 6 months. Two hundred and thirty one milk samples were collected from full-term lactating women at 1, 4, 8 and 24 weeks following birth and analysed for microbiota composition using 16S rRNA sequencing. A significant decrease in milk microbiota diversity was observed throughout the first 6 months of lactation, with the greatest difference seen between week 8 and week 24. Nine genera predominated in milk over lactation from week 1 to week 24, comprising of Staphylococcus, Streptococcus, Pseudomonas, Acinetobacter, Bifidobacterium, Mesorhizobium, Brevundimonas, Flavobacterium, and Rhodococcus; however, fluctuations in these core genera were apparent over time. There was a significant effect of stage of lactation on the microbiome, while no effect of birth mode, infant sex and maternal BMI was observed throughout lactation. Streptococcus had the highest mean relative abundance at week 1 and 24 (17.3% and 24% respectively), whereas Pseudomonas predominated at week 4 (22%) and week 8 (19%). Bifidobacterium and Lactobacillus had the highest mean relative abundance at week 4 (5% and 1.4% respectively), and occurred at a relative abundance of ≤ 1% at all other time points. A decrease in milk microbiota diversity throughout lactation was also observed. This study concluded that lactation stage was the primary driving factor in milk microbiota compositional changes over lactation from birth to 6 months, while mode of delivery was not a factor driving compositional changes throughout human lactation.
(Less)
- author
- Lyons, Katriona E.
; Shea, Carol Anne O.’
; Grimaud, Ghjuvan
LU
; Ryan, C. Anthony
; Dempsey, Eugene
; Kelly, Alan L.
; Ross, R. Paul
and Stanton, Catherine
- publishing date
- 2022-12
- type
- Contribution to journal
- publication status
- published
- in
- Scientific Reports
- volume
- 12
- issue
- 1
- article number
- 5598
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85127650007
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-022-09009-y
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2022, The Author(s).
- id
- c74f553f-19f2-4c17-8515-f66dcec6b961
- date added to LUP
- 2024-11-25 16:11:34
- date last changed
- 2025-05-13 09:42:29
@article{c74f553f-19f2-4c17-8515-f66dcec6b961,
abstract = {{<p>We analysed the human milk microbiome in a cohort of 80 lactating women and followed the dynamics in taxa over the course of lactation from birth to 6 months. Two hundred and thirty one milk samples were collected from full-term lactating women at 1, 4, 8 and 24 weeks following birth and analysed for microbiota composition using 16S rRNA sequencing. A significant decrease in milk microbiota diversity was observed throughout the first 6 months of lactation, with the greatest difference seen between week 8 and week 24. Nine genera predominated in milk over lactation from week 1 to week 24, comprising of Staphylococcus, Streptococcus, Pseudomonas, Acinetobacter, Bifidobacterium, Mesorhizobium, Brevundimonas, Flavobacterium, and Rhodococcus; however, fluctuations in these core genera were apparent over time. There was a significant effect of stage of lactation on the microbiome, while no effect of birth mode, infant sex and maternal BMI was observed throughout lactation. Streptococcus had the highest mean relative abundance at week 1 and 24 (17.3% and 24% respectively), whereas Pseudomonas predominated at week 4 (22%) and week 8 (19%). Bifidobacterium and Lactobacillus had the highest mean relative abundance at week 4 (5% and 1.4% respectively), and occurred at a relative abundance of ≤ 1% at all other time points. A decrease in milk microbiota diversity throughout lactation was also observed. This study concluded that lactation stage was the primary driving factor in milk microbiota compositional changes over lactation from birth to 6 months, while mode of delivery was not a factor driving compositional changes throughout human lactation.</p>}},
author = {{Lyons, Katriona E. and Shea, Carol Anne O.’ and Grimaud, Ghjuvan and Ryan, C. Anthony and Dempsey, Eugene and Kelly, Alan L. and Ross, R. Paul and Stanton, Catherine}},
issn = {{2045-2322}},
language = {{eng}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{The human milk microbiome aligns with lactation stage and not birth mode}},
url = {{http://dx.doi.org/10.1038/s41598-022-09009-y}},
doi = {{10.1038/s41598-022-09009-y}},
volume = {{12}},
year = {{2022}},
}