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Unraveling human aqp5-pip molecular interaction and effect on aqp5 salivary glands localization in ss patients

Chivasso, Clara ; Nesverova, Veronika LU ; Järvå, Michael ; Blanchard, Anne ; Rose, Kristie L. ; Öberg, Fredrik Kryh ; Wang, Zhen ; Martin, Maud ; Lhotellerie, Florent and Zindy, Egor , et al. (2021) In Cells 10(8).
Abstract

Saliva secretion requires effective translocation of aquaporin 5 (AQP5) water channel to the salivary glands (SGs) acinar apical membrane. Patients with Sjögren’s syndrome (SS) display abnormal AQP5 localization within acinar cells from SGs that correlate with sicca manifestation and glands hypofunction. Several proteins such as Prolactin-inducible protein (PIP) may regulate AQP5 trafficking as observed in lacrimal glands from mice. However, the role of the AQP5-PIP complex remains poorly understood. In the present study, we show that PIP interacts with AQP5 in vitro and in mice as well as in human SGs and that PIP misexpression correlates with an altered AQP5 distribution at the acinar apical membrane in PIP knockout mice and SS hMSG.... (More)

Saliva secretion requires effective translocation of aquaporin 5 (AQP5) water channel to the salivary glands (SGs) acinar apical membrane. Patients with Sjögren’s syndrome (SS) display abnormal AQP5 localization within acinar cells from SGs that correlate with sicca manifestation and glands hypofunction. Several proteins such as Prolactin-inducible protein (PIP) may regulate AQP5 trafficking as observed in lacrimal glands from mice. However, the role of the AQP5-PIP complex remains poorly understood. In the present study, we show that PIP interacts with AQP5 in vitro and in mice as well as in human SGs and that PIP misexpression correlates with an altered AQP5 distribution at the acinar apical membrane in PIP knockout mice and SS hMSG. Furthermore, our data show that the protein-protein interaction involves the AQP5 C-terminus and the N-terminal of PIP (one molecule of PIP per AQP5 tetramer). In conclusion, our findings highlight for the first time the role of PIP as a protein controlling AQP5 localization in human salivary glands but extend beyond due to the PIP-AQP5 interaction described in lung and breast cancers.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aquaporin-5, Prolactin-inducible protein, Salivary gland, Sjögren’s syndrome
in
Cells
volume
10
issue
8
article number
2108
publisher
MDPI AG
external identifiers
  • pmid:34440877
  • scopus:85113491858
ISSN
2073-4409
DOI
10.3390/cells10082108
language
English
LU publication?
yes
id
c757bbca-50a0-4624-b1c4-6392fbf44368
date added to LUP
2022-03-22 15:36:38
date last changed
2024-04-25 21:38:52
@article{c757bbca-50a0-4624-b1c4-6392fbf44368,
  abstract     = {{<p>Saliva secretion requires effective translocation of aquaporin 5 (AQP5) water channel to the salivary glands (SGs) acinar apical membrane. Patients with Sjögren’s syndrome (SS) display abnormal AQP5 localization within acinar cells from SGs that correlate with sicca manifestation and glands hypofunction. Several proteins such as Prolactin-inducible protein (PIP) may regulate AQP5 trafficking as observed in lacrimal glands from mice. However, the role of the AQP5-PIP complex remains poorly understood. In the present study, we show that PIP interacts with AQP5 in vitro and in mice as well as in human SGs and that PIP misexpression correlates with an altered AQP5 distribution at the acinar apical membrane in PIP knockout mice and SS hMSG. Furthermore, our data show that the protein-protein interaction involves the AQP5 C-terminus and the N-terminal of PIP (one molecule of PIP per AQP5 tetramer). In conclusion, our findings highlight for the first time the role of PIP as a protein controlling AQP5 localization in human salivary glands but extend beyond due to the PIP-AQP5 interaction described in lung and breast cancers.</p>}},
  author       = {{Chivasso, Clara and Nesverova, Veronika and Järvå, Michael and Blanchard, Anne and Rose, Kristie L. and Öberg, Fredrik Kryh and Wang, Zhen and Martin, Maud and Lhotellerie, Florent and Zindy, Egor and Junqueira, Bruna and Leroy, Karelle and Vanhollebeke, Benoit and Delforge, Valérie and Bolaky, Nargis and Perret, Jason and Soyfoo, Muhammad Shahnawaz and Moscato, Stefania and Baldini, Chiara and Chaumont, François and Mattii, Letizia and Schey, Kevin L. and Myal, Yvonne and Törnroth-Horsefield, Susanna and Delporte, Christine}},
  issn         = {{2073-4409}},
  keywords     = {{Aquaporin-5; Prolactin-inducible protein; Salivary gland; Sjögren’s syndrome}},
  language     = {{eng}},
  number       = {{8}},
  publisher    = {{MDPI AG}},
  series       = {{Cells}},
  title        = {{Unraveling human aqp5-pip molecular interaction and effect on aqp5 salivary glands localization in ss patients}},
  url          = {{http://dx.doi.org/10.3390/cells10082108}},
  doi          = {{10.3390/cells10082108}},
  volume       = {{10}},
  year         = {{2021}},
}