Plasmid deficiency in urogenital isolates of Chlamydia trachomatis reduces infectivity and virulence in a mouse model
(2014) In Pathogens and Disease 70(1). p.61-69- Abstract
- We hypothesized that the plasmid of urogenital isolates of Chlamydia trachomatis would modulate infectivity and virulence in a mouse model. To test this hypothesis, we infected female mice in the respiratory or urogenital tract with graded doses of a human urogenital isolate of C.trachomatis, serovar F, possessing the cognate plasmid. For comparison, we inoculated mice with a plasmid-free serovar F isolate. Following urogenital inoculation, the plasmid-free isolate displayed significantly reduced infectivity compared with the wild-type strain with the latter yielding a 17-fold lower infectious dose to yield 50% infection. When inoculated via the respiratory tract, the plasmid-free isolate exhibited reduced infectivity and virulence (as... (More)
- We hypothesized that the plasmid of urogenital isolates of Chlamydia trachomatis would modulate infectivity and virulence in a mouse model. To test this hypothesis, we infected female mice in the respiratory or urogenital tract with graded doses of a human urogenital isolate of C.trachomatis, serovar F, possessing the cognate plasmid. For comparison, we inoculated mice with a plasmid-free serovar F isolate. Following urogenital inoculation, the plasmid-free isolate displayed significantly reduced infectivity compared with the wild-type strain with the latter yielding a 17-fold lower infectious dose to yield 50% infection. When inoculated via the respiratory tract, the plasmid-free isolate exhibited reduced infectivity and virulence (as measured by weight change) when compared to the wild-type isolate. Further, differences in infectivity, but not in virulence were observed in a C.trachomatis, serovar E isolate with a deletion within the plasmid coding sequence 1 when compared to a serovar E isolate with no mutations in the plasmid. We conclude that plasmid loss reduces virulence and infectivity in this mouse model. These findings further support a role for the chlamydial plasmid in infectivity and virulence in vivo. (Less)
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https://lup.lub.lu.se/record/4376455
- author
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Chlamydia, plasmid, mouse, virulence, infection, genome
- in
- Pathogens and Disease
- volume
- 70
- issue
- 1
- pages
- 61 - 69
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000331213000007
- scopus:84901995326
- pmid:24022847
- ISSN
- 2049-632X
- DOI
- 10.1111/2049-632X.12086
- language
- English
- LU publication?
- yes
- id
- c79558a4-fa12-46b9-839a-cdfc497de05e (old id 4376455)
- date added to LUP
- 2016-04-01 09:54:32
- date last changed
- 2022-01-25 17:52:47
@article{c79558a4-fa12-46b9-839a-cdfc497de05e,
abstract = {{We hypothesized that the plasmid of urogenital isolates of Chlamydia trachomatis would modulate infectivity and virulence in a mouse model. To test this hypothesis, we infected female mice in the respiratory or urogenital tract with graded doses of a human urogenital isolate of C.trachomatis, serovar F, possessing the cognate plasmid. For comparison, we inoculated mice with a plasmid-free serovar F isolate. Following urogenital inoculation, the plasmid-free isolate displayed significantly reduced infectivity compared with the wild-type strain with the latter yielding a 17-fold lower infectious dose to yield 50% infection. When inoculated via the respiratory tract, the plasmid-free isolate exhibited reduced infectivity and virulence (as measured by weight change) when compared to the wild-type isolate. Further, differences in infectivity, but not in virulence were observed in a C.trachomatis, serovar E isolate with a deletion within the plasmid coding sequence 1 when compared to a serovar E isolate with no mutations in the plasmid. We conclude that plasmid loss reduces virulence and infectivity in this mouse model. These findings further support a role for the chlamydial plasmid in infectivity and virulence in vivo.}},
author = {{Sigar, Ira M. and Schripsema, Justin H. and Wang, Yibing and Clarke, Ian N. and Cutcliffe, Lesley T. and Seth-Smith, Helena M. B. and Thomson, Nicholas R. and Bjartling, Carina and Unemo, Magnus and Persson, Kenneth and Ramsey, Kyle H.}},
issn = {{2049-632X}},
keywords = {{Chlamydia; plasmid; mouse; virulence; infection; genome}},
language = {{eng}},
number = {{1}},
pages = {{61--69}},
publisher = {{Wiley-Blackwell}},
series = {{Pathogens and Disease}},
title = {{Plasmid deficiency in urogenital isolates of Chlamydia trachomatis reduces infectivity and virulence in a mouse model}},
url = {{http://dx.doi.org/10.1111/2049-632X.12086}},
doi = {{10.1111/2049-632X.12086}},
volume = {{70}},
year = {{2014}},
}