In vitro stimulation of insulin release by non-metabolizable, transport-specific amino acids
Christensen, Halvor N. ; Hellman, Bo ; Lernmark, Åke LU
; Sehlin, Janove
; Tager, Howard S.
and Täljedal, Inge Bert
(1971)
In BBA - Biomembranes
241(2).
p.341-348
- Abstract
The insulin-releasing ability and uptake characteristics of non-metabolizable, transport-specific amino acids were studied in an in vitro system, using microdissected pancreatic islets with more than 90% β-cells. Among the four stereoisomers of 2-aminobicyclo(2,2,1)heptane-2-carboxylic acid (BCH), only the b(-) form stimulated insulin release. This isomer is known as a specific substrate for transport system l in other cells. It was rapidly taken up by the islet cells and stimulated insulin release both in the presence and in the absence of glucose. 4-Amino-1-guanylpiperidine-4-carboxylic acid (GPA), a substrate for cationic transport systems, stimulated insulin release in the presence but not in the absence of glucose. In this respect... (More)
The insulin-releasing ability and uptake characteristics of non-metabolizable, transport-specific amino acids were studied in an in vitro system, using microdissected pancreatic islets with more than 90% β-cells. Among the four stereoisomers of 2-aminobicyclo(2,2,1)heptane-2-carboxylic acid (BCH), only the b(-) form stimulated insulin release. This isomer is known as a specific substrate for transport system l in other cells. It was rapidly taken up by the islet cells and stimulated insulin release both in the presence and in the absence of glucose. 4-Amino-1-guanylpiperidine-4-carboxylic acid (GPA), a substrate for cationic transport systems, stimulated insulin release in the presence but not in the absence of glucose. In this respect GPA is similar to arginine. Like arginine, GPA also accumulated in the islet cells to yield distribution ratios well above unity. The results are consonant with the previous hypothesis that amino acids stimulate insulin release by binding to specific transport molecules.
(Less)
- author
- Christensen, Halvor N.
; Hellman, Bo
; Lernmark, Åke
LU
; Sehlin, Janove
; Tager, Howard S.
and Täljedal, Inge Bert
- publishing date
- 1971-08-13
- type
- Contribution to journal
- publication status
- published
- in
- BBA - Biomembranes
- volume
- 241
- issue
- 2
- pages
- 341 - 348
- publisher
- Elsevier
- external identifiers
-
- pmid:4110546
- scopus:0015222032
- ISSN
- 0005-2736
- DOI
- 10.1016/0005-2736(71)90034-4
- language
- English
- LU publication?
- no
- id
- c7b31488-4e81-452e-9c1a-ac2851335b1a
- date added to LUP
- 2019-09-18 12:25:28
- date last changed
- 2024-03-13 08:19:43
@article{c7b31488-4e81-452e-9c1a-ac2851335b1a,
abstract = {{<p>The insulin-releasing ability and uptake characteristics of non-metabolizable, transport-specific amino acids were studied in an in vitro system, using microdissected pancreatic islets with more than 90% β-cells. Among the four stereoisomers of 2-aminobicyclo(2,2,1)heptane-2-carboxylic acid (BCH), only the b(-) form stimulated insulin release. This isomer is known as a specific substrate for transport system l in other cells. It was rapidly taken up by the islet cells and stimulated insulin release both in the presence and in the absence of glucose. 4-Amino-1-guanylpiperidine-4-carboxylic acid (GPA), a substrate for cationic transport systems, stimulated insulin release in the presence but not in the absence of glucose. In this respect GPA is similar to arginine. Like arginine, GPA also accumulated in the islet cells to yield distribution ratios well above unity. The results are consonant with the previous hypothesis that amino acids stimulate insulin release by binding to specific transport molecules.</p>}},
author = {{Christensen, Halvor N. and Hellman, Bo and Lernmark, Åke and Sehlin, Janove and Tager, Howard S. and Täljedal, Inge Bert}},
issn = {{0005-2736}},
language = {{eng}},
month = {{08}},
number = {{2}},
pages = {{341--348}},
publisher = {{Elsevier}},
series = {{BBA - Biomembranes}},
title = {{In vitro stimulation of insulin release by non-metabolizable, transport-specific amino acids}},
url = {{http://dx.doi.org/10.1016/0005-2736(71)90034-4}},
doi = {{10.1016/0005-2736(71)90034-4}},
volume = {{241}},
year = {{1971}},
}