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Stoichiometric theory shapes enzyme kinetics in paddy bulk soil but not in rhizosphere soil

Liu, Yuhuai ; Shahbaz, Muhammad LU ; Fang, Yunying ; Li, Baozhen ; Wei, Xiaomeng ; Zhu, Zhenke ; Lynn, Tin Mar ; Lu, Shunbao ; Shibistova, Olga and Wu, Jinshui , et al. (2022) In Land Degradation and Development 33(2). p.246-256
Abstract
The available carbon (C) to phosphorus (P) ratio in soil is regulated by extracellular hydrolases for C and P acquisition by microbes and plants. However, the stoichiometric relationship between acquiring C and P in paddy rhizosphere and bulk soils remains unclear. The objective was to explore the underlying mechanisms of C and P acquisition stoichiometry in rhizosphere and bulk soils in response to P fertilization and cellulose addition. Amendment with either cellulose or P separately caused a significant increase in the maximal velocity (Vmax) of C acquisition enzymes (β-1,4-glucosidase and β-cellobiohydrolase) but decreased that of P acquisition enzymes (acid and alkaline phosphomonoesterases) in bulk soil. In contrast, lower Vmax... (More)
The available carbon (C) to phosphorus (P) ratio in soil is regulated by extracellular hydrolases for C and P acquisition by microbes and plants. However, the stoichiometric relationship between acquiring C and P in paddy rhizosphere and bulk soils remains unclear. The objective was to explore the underlying mechanisms of C and P acquisition stoichiometry in rhizosphere and bulk soils in response to P fertilization and cellulose addition. Amendment with either cellulose or P separately caused a significant increase in the maximal velocity (Vmax) of C acquisition enzymes (β-1,4-glucosidase and β-cellobiohydrolase) but decreased that of P acquisition enzymes (acid and alkaline phosphomonoesterases) in bulk soil. In contrast, lower Vmax values of C and P acquisition enzymes were observed in rhizosphere soil than in bulk soil. The co-application of cellulose and P increased the Vmax of P acquisition enzymes in rhizosphere soil but decreased that of only alkaline phosphomonoesterase in bulk soil. Results show that P availability and labile-C content co-regulated the P/C acquisition ratio, and two inverse linear relationships were observed. Specifically, the P/C acquisition ratio was negatively related to both the dissolved organic C/Olsen-P ratio and the microbial biomass C/P ratio in rhizosphere soil. However, the P/C acquisition ratio was positively related to both the dissolved organic C/Olsen-P ratio and the microbial biomass C/P ratio in bulk soil. Overall, microbes mineralized less organic P to acquire P in paddy soil rhizosphere (i.e. containing higher labile-C) than in bulk soil (i.e. having lower labile-C contents). (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Land Degradation and Development
volume
33
issue
2
pages
246 - 256
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:85118997692
ISSN
1099-145X
DOI
10.1002/ldr.4141
language
English
LU publication?
yes
id
c7d9d133-d824-43f5-b0ca-660879480b1a
date added to LUP
2021-11-01 11:25:07
date last changed
2023-05-16 13:11:21
@article{c7d9d133-d824-43f5-b0ca-660879480b1a,
  abstract     = {{The available carbon (C) to phosphorus (P) ratio in soil is regulated by extracellular hydrolases for C and P acquisition by microbes and plants. However, the stoichiometric relationship between acquiring C and P in paddy rhizosphere and bulk soils remains unclear. The objective was to explore the underlying mechanisms of C and P acquisition stoichiometry in rhizosphere and bulk soils in response to P fertilization and cellulose addition. Amendment with either cellulose or P separately caused a significant increase in the maximal velocity (Vmax) of C acquisition enzymes (β-1,4-glucosidase and β-cellobiohydrolase) but decreased that of P acquisition enzymes (acid and alkaline phosphomonoesterases) in bulk soil. In contrast, lower Vmax values of C and P acquisition enzymes were observed in rhizosphere soil than in bulk soil. The co-application of cellulose and P increased the Vmax of P acquisition enzymes in rhizosphere soil but decreased that of only alkaline phosphomonoesterase in bulk soil. Results show that P availability and labile-C content co-regulated the P/C acquisition ratio, and two inverse linear relationships were observed. Specifically, the P/C acquisition ratio was negatively related to both the dissolved organic C/Olsen-P ratio and the microbial biomass C/P ratio in rhizosphere soil. However, the P/C acquisition ratio was positively related to both the dissolved organic C/Olsen-P ratio and the microbial biomass C/P ratio in bulk soil. Overall, microbes mineralized less organic P to acquire P in paddy soil rhizosphere (i.e. containing higher labile-C) than in bulk soil (i.e. having lower labile-C contents).}},
  author       = {{Liu, Yuhuai and Shahbaz, Muhammad and Fang, Yunying and Li, Baozhen and Wei, Xiaomeng and Zhu, Zhenke and Lynn, Tin Mar and Lu, Shunbao and Shibistova, Olga and Wu, Jinshui and Guggenberger, Georg and Ge, Tida}},
  issn         = {{1099-145X}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{246--256}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Land Degradation and Development}},
  title        = {{Stoichiometric theory shapes enzyme kinetics in paddy bulk soil but not in rhizosphere soil}},
  url          = {{http://dx.doi.org/10.1002/ldr.4141}},
  doi          = {{10.1002/ldr.4141}},
  volume       = {{33}},
  year         = {{2022}},
}