Microwave-assisted xylanase reaction : impact in the production of prebiotic xylooligosaccharides
Mobarec, Hugo ; Villagomez, Juan R V LU ; Nordberg Karlsson, Eva LU
and Linares-Pastén, Javier A.
LU
(2021)
In RSC Advances
11(20).
p.11882-11888
- Abstract
The enzymatic production of prebiotic xylooligosaccharides (XOS) has become an attractive way to valorise lignocellulosic biomass. However, despite numerous xylanases reported for potential use in the production of XOS, most of the family GH10 also produce xylose. This monosaccharide can negatively affect the selectivity to stimulate the growth of intestinal microorganisms beneficial to human health. In this work, thermostable alkali-tolerant xylanase (BhXyn10A) from Bacillus halodurans S7 has been used to produce XOS under conventional convective heat transfer and microwave radiation. The microwave-assisted reaction markedly decreases the xylose content in the hydrolysates and significantly increases the yield of XOS, compared to... (More)
The enzymatic production of prebiotic xylooligosaccharides (XOS) has become an attractive way to valorise lignocellulosic biomass. However, despite numerous xylanases reported for potential use in the production of XOS, most of the family GH10 also produce xylose. This monosaccharide can negatively affect the selectivity to stimulate the growth of intestinal microorganisms beneficial to human health. In this work, thermostable alkali-tolerant xylanase (BhXyn10A) from Bacillus halodurans S7 has been used to produce XOS under conventional convective heat transfer and microwave radiation. The microwave-assisted reaction markedly decreases the xylose content in the hydrolysates and significantly increases the yield of XOS, compared to conventional heating. Molecular dynamics simulations of BhXyn10A have shown increased fluctuations of the amino acids of the aglycone subsites suggesting that these subsites can determine the production of xylose. Thus, microwave heating could affect the amino acid fluctuations in the aglycone subsites reducing the xylose formation. These findings open up new avenues in enzyme technology for the production of XOS.
(Less)
- author
- Mobarec, Hugo
; Villagomez, Juan R V
LU
; Nordberg Karlsson, Eva
LU
and Linares-Pastén, Javier A.
LU
- organization
- publishing date
- 2021-03-23
- type
- Contribution to journal
- publication status
- published
- subject
- in
- RSC Advances
- volume
- 11
- issue
- 20
- pages
- 7 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:85103506270
- pmid:35423774
- pmid:35423774
- ISSN
- 2046-2069
- DOI
- 10.1039/D1RA00449B
- language
- English
- LU publication?
- yes
- id
- c829025b-3865-46c4-89cb-536dabc3be71
- date added to LUP
- 2021-04-08 19:09:11
- date last changed
- 2024-06-15 09:34:32
@article{c829025b-3865-46c4-89cb-536dabc3be71,
abstract = {{<p>The enzymatic production of prebiotic xylooligosaccharides (XOS) has become an attractive way to valorise lignocellulosic biomass. However, despite numerous xylanases reported for potential use in the production of XOS, most of the family GH10 also produce xylose. This monosaccharide can negatively affect the selectivity to stimulate the growth of intestinal microorganisms beneficial to human health. In this work, thermostable alkali-tolerant xylanase (BhXyn10A) from Bacillus halodurans S7 has been used to produce XOS under conventional convective heat transfer and microwave radiation. The microwave-assisted reaction markedly decreases the xylose content in the hydrolysates and significantly increases the yield of XOS, compared to conventional heating. Molecular dynamics simulations of BhXyn10A have shown increased fluctuations of the amino acids of the aglycone subsites suggesting that these subsites can determine the production of xylose. Thus, microwave heating could affect the amino acid fluctuations in the aglycone subsites reducing the xylose formation. These findings open up new avenues in enzyme technology for the production of XOS.</p>}},
author = {{Mobarec, Hugo and Villagomez, Juan R V and Nordberg Karlsson, Eva and Linares-Pastén, Javier A.}},
issn = {{2046-2069}},
language = {{eng}},
month = {{03}},
number = {{20}},
pages = {{11882--11888}},
publisher = {{Royal Society of Chemistry}},
series = {{RSC Advances}},
title = {{Microwave-assisted xylanase reaction : impact in the production of prebiotic xylooligosaccharides}},
url = {{http://dx.doi.org/10.1039/D1RA00449B}},
doi = {{10.1039/D1RA00449B}},
volume = {{11}},
year = {{2021}},
}