Preparation of a portable point-of-care in vitro diagnostic system, for quantification of canine C-reactive protein, based on a magnetic two-site immunoassay.
(2013) In Analytical and Bioanalytical Chemistry 405(18). p.6001-6007- Abstract
- In this study, characterization of the binding kinetics and optimization of a magnetic permeability based point-of-care (POC) immunoassay system for quantification of canine C-reactive protein (cCRP) is described. The reagent is based on a two-site heterogeneous immunoassay system utilizing conjugated superparamagnetic nanoparticles (SPION) and silica particles, both particles carrying covalently linked antibodies directed to the cCRP analyte. Detection is carried out using a magnetic permeability-based small instrument, adjusted in order to apply it in a POC setting near the patients. The kinetic parameters are characterized and applied in the final design of the assay system. In the cCRP system studied, 90 % of the binding between... (More)
- In this study, characterization of the binding kinetics and optimization of a magnetic permeability based point-of-care (POC) immunoassay system for quantification of canine C-reactive protein (cCRP) is described. The reagent is based on a two-site heterogeneous immunoassay system utilizing conjugated superparamagnetic nanoparticles (SPION) and silica particles, both particles carrying covalently linked antibodies directed to the cCRP analyte. Detection is carried out using a magnetic permeability-based small instrument, adjusted in order to apply it in a POC setting near the patients. The kinetic parameters are characterized and applied in the final design of the assay system. In the cCRP system studied, 90 % of the binding between immobilized solid-phase silica antibody and cCRP is complete after only 15 s, and 30 s for the binding between the antibody on the SPION and the bound cCRP on the silica particle. Additionally, the binding rate constants are determined to be 149 and 30 M(-1)s(-1), respectively. The analytical sensitivity, clinical sensitivity, and imprecision verifies the clinical usefulness of the system. Also, quantification of cCRP, using the system described, in dog clinical samples from mixed breeds shows a high correlation to a commercially available comparative cCRP ELISA system (y = 0.98 × +3.2, R (2) = 0.98, n = 47). The immunoassay system described can thus provide the veterinarian a valuable tool for rapid diagnosis and monitoring of inflammatory diseases in dogs in a setting near the patients. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3804667
- author
- Ibraimi, Filiz LU ; Ekberg, Björn ; Kriz, Dario ; Danielsson, Gertrud and Bülow, Leif LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical and Bioanalytical Chemistry
- volume
- 405
- issue
- 18
- pages
- 6001 - 6007
- publisher
- Springer
- external identifiers
-
- wos:000321065900014
- pmid:23660695
- scopus:84879793906
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-013-7032-9
- language
- English
- LU publication?
- yes
- id
- c83d6903-8437-4c0e-8df0-85aef099d00f (old id 3804667)
- date added to LUP
- 2016-04-01 10:28:43
- date last changed
- 2022-04-04 18:30:29
@article{c83d6903-8437-4c0e-8df0-85aef099d00f,
abstract = {{In this study, characterization of the binding kinetics and optimization of a magnetic permeability based point-of-care (POC) immunoassay system for quantification of canine C-reactive protein (cCRP) is described. The reagent is based on a two-site heterogeneous immunoassay system utilizing conjugated superparamagnetic nanoparticles (SPION) and silica particles, both particles carrying covalently linked antibodies directed to the cCRP analyte. Detection is carried out using a magnetic permeability-based small instrument, adjusted in order to apply it in a POC setting near the patients. The kinetic parameters are characterized and applied in the final design of the assay system. In the cCRP system studied, 90 % of the binding between immobilized solid-phase silica antibody and cCRP is complete after only 15 s, and 30 s for the binding between the antibody on the SPION and the bound cCRP on the silica particle. Additionally, the binding rate constants are determined to be 149 and 30 M(-1)s(-1), respectively. The analytical sensitivity, clinical sensitivity, and imprecision verifies the clinical usefulness of the system. Also, quantification of cCRP, using the system described, in dog clinical samples from mixed breeds shows a high correlation to a commercially available comparative cCRP ELISA system (y = 0.98 × +3.2, R (2) = 0.98, n = 47). The immunoassay system described can thus provide the veterinarian a valuable tool for rapid diagnosis and monitoring of inflammatory diseases in dogs in a setting near the patients.}},
author = {{Ibraimi, Filiz and Ekberg, Björn and Kriz, Dario and Danielsson, Gertrud and Bülow, Leif}},
issn = {{1618-2642}},
language = {{eng}},
number = {{18}},
pages = {{6001--6007}},
publisher = {{Springer}},
series = {{Analytical and Bioanalytical Chemistry}},
title = {{Preparation of a portable point-of-care in vitro diagnostic system, for quantification of canine C-reactive protein, based on a magnetic two-site immunoassay.}},
url = {{http://dx.doi.org/10.1007/s00216-013-7032-9}},
doi = {{10.1007/s00216-013-7032-9}},
volume = {{405}},
year = {{2013}},
}