Advanced

An electrical impedance indicator to assess electropermeabilisation in tissue, a preliminary study

Blad, B. LU and Persson, B. LU (2005) In ITBM-RBM 26(5-6). p.328-332
Abstract

A controlled pulsed electrical field applied to a living tissue is believed to cause a structural reorganisation in the lipid bilayer of the cell membranes. This electropermeabilisation or electroporation process is used for opening up the cell membrane in order to get access to the cell. An electrical impedance tissue model that incorporates electroporation is presented. From this model a bio-impedance indicator is proposed that assesses the pore generation in the cell membranes. The indicator was determined during and after the electroporation. An impedance measurement device was designed and calibrated. This device was used for bio-impedance measurements on living rat tie muscles in relation with electroporation. The result from... (More)

A controlled pulsed electrical field applied to a living tissue is believed to cause a structural reorganisation in the lipid bilayer of the cell membranes. This electropermeabilisation or electroporation process is used for opening up the cell membrane in order to get access to the cell. An electrical impedance tissue model that incorporates electroporation is presented. From this model a bio-impedance indicator is proposed that assesses the pore generation in the cell membranes. The indicator was determined during and after the electroporation. An impedance measurement device was designed and calibrated. This device was used for bio-impedance measurements on living rat tie muscles in relation with electroporation. The result from these measurements showed that it was possible to observe both openings and closings of the cell membranes during and after electroporation. Besides impedance measurements could be used for determining whether the electroporation is reversible or irreversible.

(Less)
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bio-impedance, Electropermeabilisation, Tissue
in
ITBM-RBM
volume
26
issue
5-6
pages
5 pages
publisher
Elsevier Masson SAS
external identifiers
  • scopus:29044443613
ISSN
1297-9562
DOI
10.1016/j.rbmret.2005.02.004
language
English
LU publication?
yes
id
c85a5d7e-99d0-49a0-90c8-872e629bd176
date added to LUP
2020-05-07 21:00:27
date last changed
2021-01-04 14:45:29
@article{c85a5d7e-99d0-49a0-90c8-872e629bd176,
  abstract     = {<p>A controlled pulsed electrical field applied to a living tissue is believed to cause a structural reorganisation in the lipid bilayer of the cell membranes. This electropermeabilisation or electroporation process is used for opening up the cell membrane in order to get access to the cell. An electrical impedance tissue model that incorporates electroporation is presented. From this model a bio-impedance indicator is proposed that assesses the pore generation in the cell membranes. The indicator was determined during and after the electroporation. An impedance measurement device was designed and calibrated. This device was used for bio-impedance measurements on living rat tie muscles in relation with electroporation. The result from these measurements showed that it was possible to observe both openings and closings of the cell membranes during and after electroporation. Besides impedance measurements could be used for determining whether the electroporation is reversible or irreversible.</p>},
  author       = {Blad, B. and Persson, B.},
  issn         = {1297-9562},
  language     = {eng},
  month        = {10},
  number       = {5-6},
  pages        = {328--332},
  publisher    = {Elsevier Masson SAS},
  series       = {ITBM-RBM},
  title        = {An electrical impedance indicator to assess electropermeabilisation in tissue, a preliminary study},
  url          = {http://dx.doi.org/10.1016/j.rbmret.2005.02.004},
  doi          = {10.1016/j.rbmret.2005.02.004},
  volume       = {26},
  year         = {2005},
}