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Bovine alpha 1-microglobulin/bikunin. Isolation and characterization of liver cDNA and urinary alpha 1-microglobulin

Lindqvist, A LU and Akerström, B LU (1996) In Biochimica et biophysica acta 1306(1). p.98-106
Abstract

cDNA coding for alpha 1-microglobulin, an immunoregulatory plasmaprotein, was isolated from bovine liver. The sequence of a total of 1258 nucleotides revealed an open reading frame of 352 amino acids. This included alpha 1-microglobulin, 182 amino acids, and bikunin, the light chain of the plasmaprotein inter-alpha-inhibitor, 147 amino acids. The two proteins were connected by a basic tetrapeptide, R-A-R-R, which conforms to the consensus sequence recognized by endoproteolytic cleavage enzymes. The deduced amino acid sequence showed a high degree of identity with alpha 1-microglobulin and bikunin sequences from other species, and the alpha 1-microglobulin part displayed sequence motifs typical for members of the lipocalin protein... (More)

cDNA coding for alpha 1-microglobulin, an immunoregulatory plasmaprotein, was isolated from bovine liver. The sequence of a total of 1258 nucleotides revealed an open reading frame of 352 amino acids. This included alpha 1-microglobulin, 182 amino acids, and bikunin, the light chain of the plasmaprotein inter-alpha-inhibitor, 147 amino acids. The two proteins were connected by a basic tetrapeptide, R-A-R-R, which conforms to the consensus sequence recognized by endoproteolytic cleavage enzymes. The deduced amino acid sequence showed a high degree of identity with alpha 1-microglobulin and bikunin sequences from other species, and the alpha 1-microglobulin part displayed sequence motifs typical for members of the lipocalin protein superfamily. A single alpha 1-microglobulin/bikunin mRNA with a size of around 1300 nt was found in bovine liver. The mature alpha 1-microglobulin protein was isolated from bovine urine, and partly characterized. It was found to be a globular molecule with an apparent molecular weight of 23,300, containing one N-linked and at least on O-linked oligosaccharide, one intra-chain disulfide bridge and an electrophoretic heterogeniety with a pI-value of 4.1-5.2.

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Contribution to journal
publication status
published
subject
keywords
Alpha-Globulins/genetics, Amino Acid Sequence, Animals, Base Sequence, Cattle/genetics, Cricetinae, DNA, Complementary/genetics, Female, Genes, Gerbillinae, Glycoproteins/genetics, Glycosylation, Guinea Pigs, Humans, Liver/metabolism, Membrane Glycoproteins, Molecular Sequence Data, Molecular Weight, Open Reading Frames, Pregnancy, Protein Processing, Post-Translational, Rabbits, Sequence Alignment, Sequence Homology, Amino Acid, Species Specificity, Trypsin Inhibitor, Kunitz Soybean, Urine/chemistry
in
Biochimica et biophysica acta
volume
1306
issue
1
pages
98 - 106
publisher
Elsevier
external identifiers
  • pmid:8611630
ISSN
0006-3002
DOI
10.1016/0167-4781(95)00235-9
language
English
LU publication?
yes
id
c8635079-89db-415c-832e-7960a7381a06
date added to LUP
2019-05-22 10:19:01
date last changed
2019-11-21 04:01:03
@article{c8635079-89db-415c-832e-7960a7381a06,
  abstract     = {<p>cDNA coding for alpha 1-microglobulin, an immunoregulatory plasmaprotein, was isolated from bovine liver. The sequence of a total of 1258 nucleotides revealed an open reading frame of 352 amino acids. This included alpha 1-microglobulin, 182 amino acids, and bikunin, the light chain of the plasmaprotein inter-alpha-inhibitor, 147 amino acids. The two proteins were connected by a basic tetrapeptide, R-A-R-R, which conforms to the consensus sequence recognized by endoproteolytic cleavage enzymes. The deduced amino acid sequence showed a high degree of identity with alpha 1-microglobulin and bikunin sequences from other species, and the alpha 1-microglobulin part displayed sequence motifs typical for members of the lipocalin protein superfamily. A single alpha 1-microglobulin/bikunin mRNA with a size of around 1300 nt was found in bovine liver. The mature alpha 1-microglobulin protein was isolated from bovine urine, and partly characterized. It was found to be a globular molecule with an apparent molecular weight of 23,300, containing one N-linked and at least on O-linked oligosaccharide, one intra-chain disulfide bridge and an electrophoretic heterogeniety with a pI-value of 4.1-5.2.</p>},
  author       = {Lindqvist, A and Akerström, B},
  issn         = {0006-3002},
  language     = {eng},
  month        = {04},
  number       = {1},
  pages        = {98--106},
  publisher    = {Elsevier},
  series       = {Biochimica et biophysica acta},
  title        = {Bovine alpha 1-microglobulin/bikunin. Isolation and characterization of liver cDNA and urinary alpha 1-microglobulin},
  url          = {http://dx.doi.org/10.1016/0167-4781(95)00235-9},
  doi          = {10.1016/0167-4781(95)00235-9},
  volume       = {1306},
  year         = {1996},
}