Efficient sample preparation in immuno-matrix-assisted laser desorption/ionization mass spectrometry using acoustic trapping
(2013) In Biomicrofluidics 7(2).- Abstract
- Acoustic trapping of minute bead amounts against fluid flow allows for easy automation of multiple assay steps, using a convenient aspirate/dispense format. Here, a method based on acoustic trapping that allows sample preparation for immuno-matrix-assisted laser desorption/ionization mass spectrometry using only half a million 2.8 mu m antibody covered beads is presented. The acoustic trapping is done in 200 x 2000 mu m2 glass capillaries and provides highly efficient binding and washing conditions, as shown by complete removal of detergents and sample processing times of 5-10 min. The versatility of the method is demonstrated using an antibody against Angiotensin I (Ang I), a peptide hormone involved in hypotension. Using this model... (More)
- Acoustic trapping of minute bead amounts against fluid flow allows for easy automation of multiple assay steps, using a convenient aspirate/dispense format. Here, a method based on acoustic trapping that allows sample preparation for immuno-matrix-assisted laser desorption/ionization mass spectrometry using only half a million 2.8 mu m antibody covered beads is presented. The acoustic trapping is done in 200 x 2000 mu m2 glass capillaries and provides highly efficient binding and washing conditions, as shown by complete removal of detergents and sample processing times of 5-10 min. The versatility of the method is demonstrated using an antibody against Angiotensin I (Ang I), a peptide hormone involved in hypotension. Using this model system, the acoustic trapping was efficient in enriching Angiotensin at 400 pM spiked in plasma samples. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4798473] (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3853136
- author
- Hammarström, Björn LU ; Yan, Hong LU ; Nilsson, Johan LU and Ekström, Simon LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- acoustic applications, biological specimen preparation, molecular, biophysics, organic compounds, time of flight mass spectroscopy
- in
- Biomicrofluidics
- volume
- 7
- issue
- 2
- article number
- 024107
- publisher
- American Institute of Physics (AIP)
- external identifiers
-
- wos:000318248700009
- pmid:24404012
- scopus:84877655452
- pmid:24404012
- ISSN
- 1932-1058
- DOI
- 10.1063/1.4798473
- language
- English
- LU publication?
- yes
- id
- c8935c4c-35bc-4973-9d66-e185a440c974 (old id 3853136)
- date added to LUP
- 2016-04-01 12:57:59
- date last changed
- 2022-01-27 08:34:54
@article{c8935c4c-35bc-4973-9d66-e185a440c974,
abstract = {{Acoustic trapping of minute bead amounts against fluid flow allows for easy automation of multiple assay steps, using a convenient aspirate/dispense format. Here, a method based on acoustic trapping that allows sample preparation for immuno-matrix-assisted laser desorption/ionization mass spectrometry using only half a million 2.8 mu m antibody covered beads is presented. The acoustic trapping is done in 200 x 2000 mu m2 glass capillaries and provides highly efficient binding and washing conditions, as shown by complete removal of detergents and sample processing times of 5-10 min. The versatility of the method is demonstrated using an antibody against Angiotensin I (Ang I), a peptide hormone involved in hypotension. Using this model system, the acoustic trapping was efficient in enriching Angiotensin at 400 pM spiked in plasma samples. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4798473]}},
author = {{Hammarström, Björn and Yan, Hong and Nilsson, Johan and Ekström, Simon}},
issn = {{1932-1058}},
keywords = {{acoustic applications; biological specimen preparation; molecular; biophysics; organic compounds; time of flight mass spectroscopy}},
language = {{eng}},
number = {{2}},
publisher = {{American Institute of Physics (AIP)}},
series = {{Biomicrofluidics}},
title = {{Efficient sample preparation in immuno-matrix-assisted laser desorption/ionization mass spectrometry using acoustic trapping}},
url = {{http://dx.doi.org/10.1063/1.4798473}},
doi = {{10.1063/1.4798473}},
volume = {{7}},
year = {{2013}},
}