Immunoreactivity of recombinant human glandular kallikrein using monoclonal antibodies raised against prostate-specific antigen
(1997) In Prostate 31(2). p.84-90- Abstract
The gene encoding human glandular kallikrein (KLK2) was expressed in Escherichia coli, and the corresponding protein (hK2) was produced by fermentation. The hK2 was characterized by Western blotting and epitope map using monoclonal antibodies (MAbs) specific for another protease, prostate- specific antigen (PSA) with high structural identity (80%). MAbs that recognized three different epitopes were bound to hK2, representing 7 out of 23 MAbs tested. One epitope was localized to the sequence region around amino acid position 78, which is believed to be glycosylated in hK2. The affinities of MAbs recognizing hK2 were similar to those for PSA, suggesting that common epitopes seem to contain very conserved structures. The results may help... (More)
The gene encoding human glandular kallikrein (KLK2) was expressed in Escherichia coli, and the corresponding protein (hK2) was produced by fermentation. The hK2 was characterized by Western blotting and epitope map using monoclonal antibodies (MAbs) specific for another protease, prostate- specific antigen (PSA) with high structural identity (80%). MAbs that recognized three different epitopes were bound to hK2, representing 7 out of 23 MAbs tested. One epitope was localized to the sequence region around amino acid position 78, which is believed to be glycosylated in hK2. The affinities of MAbs recognizing hK2 were similar to those for PSA, suggesting that common epitopes seem to contain very conserved structures. The results may help in designing specific diagnostic assays for the assessment of prostate cancer.
(Less)
- author
- Eerola, Riitta ; Piironen, Timo ; Pettersson, Kim ; Lövgren, Janita LU ; Vehniäinen, Markus ; Lilja, Hans LU ; Dowell, Barry ; Lövgren, Timo and Karp, Matti
- organization
- publishing date
- 1997-05-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cancer of the prostate, cDNA, diagnosis, Escherichia coli, hK2, protease, PSA
- in
- Prostate
- volume
- 31
- issue
- 2
- pages
- 7 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0030949868
- pmid:9140120
- ISSN
- 0270-4137
- DOI
- 10.1002/(SICI)1097-0045(19970501)31:2<84::AID-PROS2>3.0.CO;2-H
- language
- English
- LU publication?
- yes
- id
- c8a9b34d-4bed-4896-a318-385917158ef2
- date added to LUP
- 2022-12-06 15:54:03
- date last changed
- 2024-04-04 13:50:27
@article{c8a9b34d-4bed-4896-a318-385917158ef2, abstract = {{<p>The gene encoding human glandular kallikrein (KLK2) was expressed in Escherichia coli, and the corresponding protein (hK2) was produced by fermentation. The hK2 was characterized by Western blotting and epitope map using monoclonal antibodies (MAbs) specific for another protease, prostate- specific antigen (PSA) with high structural identity (80%). MAbs that recognized three different epitopes were bound to hK2, representing 7 out of 23 MAbs tested. One epitope was localized to the sequence region around amino acid position 78, which is believed to be glycosylated in hK2. The affinities of MAbs recognizing hK2 were similar to those for PSA, suggesting that common epitopes seem to contain very conserved structures. The results may help in designing specific diagnostic assays for the assessment of prostate cancer.</p>}}, author = {{Eerola, Riitta and Piironen, Timo and Pettersson, Kim and Lövgren, Janita and Vehniäinen, Markus and Lilja, Hans and Dowell, Barry and Lövgren, Timo and Karp, Matti}}, issn = {{0270-4137}}, keywords = {{cancer of the prostate; cDNA; diagnosis; Escherichia coli; hK2; protease; PSA}}, language = {{eng}}, month = {{05}}, number = {{2}}, pages = {{84--90}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Prostate}}, title = {{Immunoreactivity of recombinant human glandular kallikrein using monoclonal antibodies raised against prostate-specific antigen}}, url = {{http://dx.doi.org/10.1002/(SICI)1097-0045(19970501)31:2<84::AID-PROS2>3.0.CO;2-H}}, doi = {{10.1002/(SICI)1097-0045(19970501)31:2<84::AID-PROS2>3.0.CO;2-H}}, volume = {{31}}, year = {{1997}}, }