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Immunoreactivity of recombinant human glandular kallikrein using monoclonal antibodies raised against prostate-specific antigen

Eerola, Riitta ; Piironen, Timo ; Pettersson, Kim ; Lövgren, Janita LU ; Vehniäinen, Markus ; Lilja, Hans LU orcid ; Dowell, Barry ; Lövgren, Timo and Karp, Matti (1997) In Prostate 31(2). p.84-90
Abstract

The gene encoding human glandular kallikrein (KLK2) was expressed in Escherichia coli, and the corresponding protein (hK2) was produced by fermentation. The hK2 was characterized by Western blotting and epitope map using monoclonal antibodies (MAbs) specific for another protease, prostate- specific antigen (PSA) with high structural identity (80%). MAbs that recognized three different epitopes were bound to hK2, representing 7 out of 23 MAbs tested. One epitope was localized to the sequence region around amino acid position 78, which is believed to be glycosylated in hK2. The affinities of MAbs recognizing hK2 were similar to those for PSA, suggesting that common epitopes seem to contain very conserved structures. The results may help... (More)

The gene encoding human glandular kallikrein (KLK2) was expressed in Escherichia coli, and the corresponding protein (hK2) was produced by fermentation. The hK2 was characterized by Western blotting and epitope map using monoclonal antibodies (MAbs) specific for another protease, prostate- specific antigen (PSA) with high structural identity (80%). MAbs that recognized three different epitopes were bound to hK2, representing 7 out of 23 MAbs tested. One epitope was localized to the sequence region around amino acid position 78, which is believed to be glycosylated in hK2. The affinities of MAbs recognizing hK2 were similar to those for PSA, suggesting that common epitopes seem to contain very conserved structures. The results may help in designing specific diagnostic assays for the assessment of prostate cancer.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cancer of the prostate, cDNA, diagnosis, Escherichia coli, hK2, protease, PSA
in
Prostate
volume
31
issue
2
pages
7 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:9140120
  • scopus:0030949868
ISSN
0270-4137
DOI
10.1002/(SICI)1097-0045(19970501)31:2<84::AID-PROS2>3.0.CO;2-H
language
English
LU publication?
yes
id
c8a9b34d-4bed-4896-a318-385917158ef2
date added to LUP
2022-12-06 15:54:03
date last changed
2024-04-04 13:50:27
@article{c8a9b34d-4bed-4896-a318-385917158ef2,
  abstract     = {{<p>The gene encoding human glandular kallikrein (KLK2) was expressed in Escherichia coli, and the corresponding protein (hK2) was produced by fermentation. The hK2 was characterized by Western blotting and epitope map using monoclonal antibodies (MAbs) specific for another protease, prostate- specific antigen (PSA) with high structural identity (80%). MAbs that recognized three different epitopes were bound to hK2, representing 7 out of 23 MAbs tested. One epitope was localized to the sequence region around amino acid position 78, which is believed to be glycosylated in hK2. The affinities of MAbs recognizing hK2 were similar to those for PSA, suggesting that common epitopes seem to contain very conserved structures. The results may help in designing specific diagnostic assays for the assessment of prostate cancer.</p>}},
  author       = {{Eerola, Riitta and Piironen, Timo and Pettersson, Kim and Lövgren, Janita and Vehniäinen, Markus and Lilja, Hans and Dowell, Barry and Lövgren, Timo and Karp, Matti}},
  issn         = {{0270-4137}},
  keywords     = {{cancer of the prostate; cDNA; diagnosis; Escherichia coli; hK2; protease; PSA}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{2}},
  pages        = {{84--90}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Prostate}},
  title        = {{Immunoreactivity of recombinant human glandular kallikrein using monoclonal antibodies raised against prostate-specific antigen}},
  url          = {{http://dx.doi.org/10.1002/(SICI)1097-0045(19970501)31:2<84::AID-PROS2>3.0.CO;2-H}},
  doi          = {{10.1002/(SICI)1097-0045(19970501)31:2<84::AID-PROS2>3.0.CO;2-H}},
  volume       = {{31}},
  year         = {{1997}},
}