Flow sorting of mouse pancreatic B cells by forward and orthogonal light scattering
Nielsen, O. ; Larsen, J. K. ; Christensen, I. J. and Lernmark LU
(1982)
In Cytometry
3(3).
p.177-181
- Abstract
Mouse islet cell suspensions were separated into subpopulations based on forward low angle and orthogonal scattered light. Separated fractions were analyzed for hormone content by radioimmunoassays. The pancreatic B‐cells had the highest orthogonal light scattering, while they overlapped in forward low angle light scattering with other endocrine (glucagon, pancreatic polypeptide and somatostatin containing cells) and nonendocrine cells. Living and paraformaldehyde fixed islet cell suspensions showed similar distributions of combined light scattering.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/c8dfb788-c100-4572-9eea-185e830c839f
- author
- Nielsen, O.
; Larsen, J. K.
; Christensen, I. J.
and Lernmark
LU
- publishing date
- 1982-01-01
- type
- Contribution to journal
- publication status
- published
- keywords
- flow cytometry, Insulin‐containing cells, mouse, orthogonal light scattering, subpopulation separation
- in
- Cytometry
- volume
- 3
- issue
- 3
- pages
- 5 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0020366548
- pmid:6129116
- ISSN
- 0196-4763
- DOI
- 10.1002/cyto.990030307
- language
- English
- LU publication?
- no
- id
- c8dfb788-c100-4572-9eea-185e830c839f
- date added to LUP
- 2019-09-16 14:41:44
- date last changed
- 2024-03-13 08:10:56
@article{c8dfb788-c100-4572-9eea-185e830c839f,
abstract = {{<p>Mouse islet cell suspensions were separated into subpopulations based on forward low angle and orthogonal scattered light. Separated fractions were analyzed for hormone content by radioimmunoassays. The pancreatic B‐cells had the highest orthogonal light scattering, while they overlapped in forward low angle light scattering with other endocrine (glucagon, pancreatic polypeptide and somatostatin containing cells) and nonendocrine cells. Living and paraformaldehyde fixed islet cell suspensions showed similar distributions of combined light scattering.</p>}},
author = {{Nielsen, O. and Larsen, J. K. and Christensen, I. J. and Lernmark}},
issn = {{0196-4763}},
keywords = {{flow cytometry; Insulin‐containing cells; mouse; orthogonal light scattering; subpopulation separation}},
language = {{eng}},
month = {{01}},
number = {{3}},
pages = {{177--181}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Cytometry}},
title = {{Flow sorting of mouse pancreatic B cells by forward and orthogonal light scattering}},
url = {{http://dx.doi.org/10.1002/cyto.990030307}},
doi = {{10.1002/cyto.990030307}},
volume = {{3}},
year = {{1982}},
}