Sharpening emitter localization in front of a tuned mirror
Heil, Hannah S LU
; Schreiber, Benjamin
; Götz, Ralph
; Emmerling, Monika
; Dabauvalle, Marie-Christine
; Krohne, Georg
; Höfling, Sven
; Kamp, Martin
; Sauer, Markus
and Heinze, Katrin G
(2018)
In Light, science & applications
7.
- Abstract
Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror2-4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/c97f884d-8313-401e-ad96-5dddbdf9d502
- author
- Heil, Hannah S
LU
; Schreiber, Benjamin
; Götz, Ralph
; Emmerling, Monika
; Dabauvalle, Marie-Christine
; Krohne, Georg
; Höfling, Sven
; Kamp, Martin
; Sauer, Markus
and Heinze, Katrin G
- publishing date
- 2018
- type
- Contribution to journal
- publication status
- published
- in
- Light, science & applications
- volume
- 7
- article number
- 99
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85057608807
- pmid:30534368
- ISSN
- 2047-7538
- DOI
- 10.1038/s41377-018-0104-z
- language
- English
- LU publication?
- no
- id
- c97f884d-8313-401e-ad96-5dddbdf9d502
- date added to LUP
- 2025-04-26 12:05:32
- date last changed
- 2025-04-29 03:31:57
@article{c97f884d-8313-401e-ad96-5dddbdf9d502,
abstract = {{<p>Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror2-4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.</p>}},
author = {{Heil, Hannah S and Schreiber, Benjamin and Götz, Ralph and Emmerling, Monika and Dabauvalle, Marie-Christine and Krohne, Georg and Höfling, Sven and Kamp, Martin and Sauer, Markus and Heinze, Katrin G}},
issn = {{2047-7538}},
language = {{eng}},
publisher = {{Nature Publishing Group}},
series = {{Light, science & applications}},
title = {{Sharpening emitter localization in front of a tuned mirror}},
url = {{http://dx.doi.org/10.1038/s41377-018-0104-z}},
doi = {{10.1038/s41377-018-0104-z}},
volume = {{7}},
year = {{2018}},
}