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Sharpening emitter localization in front of a tuned mirror

Heil, Hannah S LU orcid ; Schreiber, Benjamin ; Götz, Ralph ; Emmerling, Monika ; Dabauvalle, Marie-Christine ; Krohne, Georg ; Höfling, Sven ; Kamp, Martin ; Sauer, Markus and Heinze, Katrin G (2018) In Light, science & applications 7.
Abstract

Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror2-4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.

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author
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publishing date
type
Contribution to journal
publication status
published
in
Light, science & applications
volume
7
article number
99
publisher
Nature Publishing Group
external identifiers
  • pmid:30534368
  • scopus:85057608807
ISSN
2047-7538
DOI
10.1038/s41377-018-0104-z
language
English
LU publication?
no
id
c97f884d-8313-401e-ad96-5dddbdf9d502
date added to LUP
2025-04-26 12:05:32
date last changed
2025-10-14 10:50:52
@article{c97f884d-8313-401e-ad96-5dddbdf9d502,
  abstract     = {{<p>Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror2-4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.</p>}},
  author       = {{Heil, Hannah S and Schreiber, Benjamin and Götz, Ralph and Emmerling, Monika and Dabauvalle, Marie-Christine and Krohne, Georg and Höfling, Sven and Kamp, Martin and Sauer, Markus and Heinze, Katrin G}},
  issn         = {{2047-7538}},
  language     = {{eng}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Light, science & applications}},
  title        = {{Sharpening emitter localization in front of a tuned mirror}},
  url          = {{http://dx.doi.org/10.1038/s41377-018-0104-z}},
  doi          = {{10.1038/s41377-018-0104-z}},
  volume       = {{7}},
  year         = {{2018}},
}