Secreted Group IIA Phospholipase A2 Protects Humans Against the Group B Streptococcus: Experimental and Clinical Evidence.
(2013) In Journal of Infectious Diseases 208(12). p.2025-2035- Abstract
- Group B streptococcus (GBS) is a leading neonatal pathogen and a growing cause of invasive disease in the elderly, with clinical manifestations such as pneumonia and sepsis. Despite its clinical importance, little is known about innate immunity against GBS in humans. Here, we analyze the role of human group IIA secreted phospholipase A2 (sPLA2-IIA), a bactericidal enzyme induced during acute inflammation, in innate immunity against GBS. We show that clinical GBS isolates are highly sensitive to killing by sPLA2-IIA but not by human antimicrobial peptides. Using transgenic mice that express human sPLA2-IIA, we demonstrate that this enzyme is crucial for host protection against systemic infection and lung challenge by GBS. We found that... (More)
- Group B streptococcus (GBS) is a leading neonatal pathogen and a growing cause of invasive disease in the elderly, with clinical manifestations such as pneumonia and sepsis. Despite its clinical importance, little is known about innate immunity against GBS in humans. Here, we analyze the role of human group IIA secreted phospholipase A2 (sPLA2-IIA), a bactericidal enzyme induced during acute inflammation, in innate immunity against GBS. We show that clinical GBS isolates are highly sensitive to killing by sPLA2-IIA but not by human antimicrobial peptides. Using transgenic mice that express human sPLA2-IIA, we demonstrate that this enzyme is crucial for host protection against systemic infection and lung challenge by GBS. We found that acute sera from humans diagnosed with invasive GBS disease contain increased levels of sPLA2-IIA compared with normal sera from healthy individuals, indicating that GBS induces an sPLA2-IIA response in blood during human infection. We demonstrate that clinically relevant GBS strains are rapidly killed in these acute sera. We also demonstrate that the bactericidal effect is entirely due to sPLA2-IIA, showing that sPLA2-IIA might represent an important component of humoral innate immunity against GBS. Our data provide experimental and clinical evidence that sPLA2-IIA protects humans against GBS infections. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4006392
- author
- Movert, Elin LU ; Wu, Yongzheng ; Lambeau, Gérard ; Kahn, Fredrik LU ; Touqui, Lhousseine and Areschoug, Thomas LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Infectious Diseases
- volume
- 208
- issue
- 12
- pages
- 2025 - 2035
- publisher
- Oxford University Press
- external identifiers
-
- wos:000327544600013
- pmid:23901095
- scopus:84897118527
- pmid:23901095
- ISSN
- 1537-6613
- DOI
- 10.1093/infdis/jit359
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Medical Microbiology (013250400), Division of Infection Medicine (SUS) (013008000)
- id
- c9d991ed-361b-4e01-bbaf-3f96f1fc5c2a (old id 4006392)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23901095?dopt=Abstract
- date added to LUP
- 2016-04-01 10:48:15
- date last changed
- 2022-03-27 19:41:47
@article{c9d991ed-361b-4e01-bbaf-3f96f1fc5c2a, abstract = {{Group B streptococcus (GBS) is a leading neonatal pathogen and a growing cause of invasive disease in the elderly, with clinical manifestations such as pneumonia and sepsis. Despite its clinical importance, little is known about innate immunity against GBS in humans. Here, we analyze the role of human group IIA secreted phospholipase A2 (sPLA2-IIA), a bactericidal enzyme induced during acute inflammation, in innate immunity against GBS. We show that clinical GBS isolates are highly sensitive to killing by sPLA2-IIA but not by human antimicrobial peptides. Using transgenic mice that express human sPLA2-IIA, we demonstrate that this enzyme is crucial for host protection against systemic infection and lung challenge by GBS. We found that acute sera from humans diagnosed with invasive GBS disease contain increased levels of sPLA2-IIA compared with normal sera from healthy individuals, indicating that GBS induces an sPLA2-IIA response in blood during human infection. We demonstrate that clinically relevant GBS strains are rapidly killed in these acute sera. We also demonstrate that the bactericidal effect is entirely due to sPLA2-IIA, showing that sPLA2-IIA might represent an important component of humoral innate immunity against GBS. Our data provide experimental and clinical evidence that sPLA2-IIA protects humans against GBS infections.}}, author = {{Movert, Elin and Wu, Yongzheng and Lambeau, Gérard and Kahn, Fredrik and Touqui, Lhousseine and Areschoug, Thomas}}, issn = {{1537-6613}}, language = {{eng}}, number = {{12}}, pages = {{2025--2035}}, publisher = {{Oxford University Press}}, series = {{Journal of Infectious Diseases}}, title = {{Secreted Group IIA Phospholipase A2 Protects Humans Against the Group B Streptococcus: Experimental and Clinical Evidence.}}, url = {{http://dx.doi.org/10.1093/infdis/jit359}}, doi = {{10.1093/infdis/jit359}}, volume = {{208}}, year = {{2013}}, }