Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

The Apoptotic Effect of Plant Based Nanosilver in Colon Cancer Cells is a p53 Dependent Process Involving ROS and JNK Cascade

Satapathy, Shakti Ranjan LU ; Mohapatra, Purusottam LU ; Das, Dipon ; Siddharth, Sumit and Kundu, Chanakya Nath (2015) In Pathology and Oncology Research 21(2). p.11-405
Abstract

Here, we report the p53 dependent mitochondria-mediated apoptotic mechanism of plant derived silver-nanoparticle (PD-AgNPs) in colorectal cancer cells (CRCs). PD-AgNPs was synthesized by reduction of AgNO3 with leaf extract of a medicinal plant periwinkle and characterized. Uptake of PD-AgNPs (ξ - 2.52 ± 4.31 mV) in HCT116 cells was 3 fold higher in comparison to synthetic AgNPs (ξ +2.293 ± 5.1 mV). A dose dependent increase in ROS production, activated JNK and decreased mitochondrial membrane potential (MMP) were noted in HCT116 but not in HCT116 p53(-/-) cells after PD-AgNP exposure. PD-AgNP-mediated apoptosis in CRCs is a p53 dependent process involving ROS and JNK cascade.

Please use this url to cite or link to this publication:
author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Antineoplastic Agents/pharmacology, Apoptosis/drug effects, Cell Line, Tumor, Cell Survival/drug effects, Colonic Neoplasms/metabolism, Cytochromes c/metabolism, Dose-Response Relationship, Drug, HCT116 Cells, Humans, MAP Kinase Signaling System/drug effects, Membrane Potential, Mitochondrial/drug effects, Mitogen-Activated Protein Kinase Kinases/metabolism, Nanoparticles, Plant Extracts/pharmacology, Reactive Oxygen Species/metabolism, Silver/pharmacology, Treatment Outcome, Tumor Suppressor Protein p53/metabolism
in
Pathology and Oncology Research
volume
21
issue
2
pages
11 - 405
publisher
Frontiers Media S. A.
external identifiers
  • pmid:25359126
  • scopus:84938086363
ISSN
1532-2807
DOI
10.1007/s12253-014-9835-1
language
English
LU publication?
no
id
ca02e5ee-fd0f-4bde-a202-749f52126d86
date added to LUP
2025-01-30 10:33:48
date last changed
2025-06-20 14:39:09
@article{ca02e5ee-fd0f-4bde-a202-749f52126d86,
  abstract     = {{<p>Here, we report the p53 dependent mitochondria-mediated apoptotic mechanism of plant derived silver-nanoparticle (PD-AgNPs) in colorectal cancer cells (CRCs). PD-AgNPs was synthesized by reduction of AgNO3 with leaf extract of a medicinal plant periwinkle and characterized. Uptake of PD-AgNPs (ξ - 2.52 ± 4.31 mV) in HCT116 cells was 3 fold higher in comparison to synthetic AgNPs (ξ +2.293 ± 5.1 mV). A dose dependent increase in ROS production, activated JNK and decreased mitochondrial membrane potential (MMP) were noted in HCT116 but not in HCT116 p53(-/-) cells after PD-AgNP exposure. PD-AgNP-mediated apoptosis in CRCs is a p53 dependent process involving ROS and JNK cascade.</p>}},
  author       = {{Satapathy, Shakti Ranjan and Mohapatra, Purusottam and Das, Dipon and Siddharth, Sumit and Kundu, Chanakya Nath}},
  issn         = {{1532-2807}},
  keywords     = {{Antineoplastic Agents/pharmacology; Apoptosis/drug effects; Cell Line, Tumor; Cell Survival/drug effects; Colonic Neoplasms/metabolism; Cytochromes c/metabolism; Dose-Response Relationship, Drug; HCT116 Cells; Humans; MAP Kinase Signaling System/drug effects; Membrane Potential, Mitochondrial/drug effects; Mitogen-Activated Protein Kinase Kinases/metabolism; Nanoparticles; Plant Extracts/pharmacology; Reactive Oxygen Species/metabolism; Silver/pharmacology; Treatment Outcome; Tumor Suppressor Protein p53/metabolism}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{11--405}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Pathology and Oncology Research}},
  title        = {{The Apoptotic Effect of Plant Based Nanosilver in Colon Cancer Cells is a p53 Dependent Process Involving ROS and JNK Cascade}},
  url          = {{http://dx.doi.org/10.1007/s12253-014-9835-1}},
  doi          = {{10.1007/s12253-014-9835-1}},
  volume       = {{21}},
  year         = {{2015}},
}