Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation
Palma-Tortosa, Sara LU ; Martínez-Curiel, Raquel LU
; Aretio-Medina, Constanza
LU
; Avaliani, Natalia
LU
and Kokaia, Zaal
LU
(2022)
In Journal of Visualized Experiments
2022(190).
- Abstract
Neurodegenerative disorders are common and heterogeneous in terms of their symptoms and cellular affectation, making their study complicated due to the lack of proper animal models that fully mimic human diseases and the poor availability of post-mortem human brain tissue. Adult human nervous tissue culture offers the possibility to study different aspects of neurological disorders. Molecular, cellular, and biochemical mechanisms could be easily addressed in this system, as well as testing and validating drugs or different treatments, such as cell-based therapies. This method combines long-term organotypic cultures of the adult human cortex, obtained from epileptic patients undergoing resective surgery, and ex vivo intracortical... (More)
Neurodegenerative disorders are common and heterogeneous in terms of their symptoms and cellular affectation, making their study complicated due to the lack of proper animal models that fully mimic human diseases and the poor availability of post-mortem human brain tissue. Adult human nervous tissue culture offers the possibility to study different aspects of neurological disorders. Molecular, cellular, and biochemical mechanisms could be easily addressed in this system, as well as testing and validating drugs or different treatments, such as cell-based therapies. This method combines long-term organotypic cultures of the adult human cortex, obtained from epileptic patients undergoing resective surgery, and ex vivo intracortical transplantation of induced pluripotent stem cell-derived cortical progenitors. This method will allow the study of cell survival, neuronal differentiation, the formation of synaptic inputs and outputs, and the electrophysiological properties of human-derived cells after transplantation into intact adult human cortical tissue. This approach is an important step prior to the development of a 3D human disease modeling platform that will bring basic research closer to the clinical translation of stem cell-based therapies for patients with different neurological disorders and allow the development of new tools for reconstructing damaged neural circuits.
(Less)
- author
- Palma-Tortosa, Sara
LU
; Martínez-Curiel, Raquel
LU
; Aretio-Medina, Constanza
LU
; Avaliani, Natalia
LU
and Kokaia, Zaal
LU
- organization
- publishing date
- 2022-12
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Visualized Experiments
- volume
- 2022
- issue
- 190
- article number
- e64234
- publisher
- JoVE
- external identifiers
-
- scopus:85144636258
- pmid:36571400
- ISSN
- 1940-087X
- DOI
- 10.3791/64234
- language
- English
- LU publication?
- yes
- id
- ca1f9864-2873-49b6-b9cb-2eafb0d59c8a
- date added to LUP
- 2023-01-05 12:35:38
- date last changed
- 2024-05-30 21:58:58
@article{ca1f9864-2873-49b6-b9cb-2eafb0d59c8a,
abstract = {{<p>Neurodegenerative disorders are common and heterogeneous in terms of their symptoms and cellular affectation, making their study complicated due to the lack of proper animal models that fully mimic human diseases and the poor availability of post-mortem human brain tissue. Adult human nervous tissue culture offers the possibility to study different aspects of neurological disorders. Molecular, cellular, and biochemical mechanisms could be easily addressed in this system, as well as testing and validating drugs or different treatments, such as cell-based therapies. This method combines long-term organotypic cultures of the adult human cortex, obtained from epileptic patients undergoing resective surgery, and ex vivo intracortical transplantation of induced pluripotent stem cell-derived cortical progenitors. This method will allow the study of cell survival, neuronal differentiation, the formation of synaptic inputs and outputs, and the electrophysiological properties of human-derived cells after transplantation into intact adult human cortical tissue. This approach is an important step prior to the development of a 3D human disease modeling platform that will bring basic research closer to the clinical translation of stem cell-based therapies for patients with different neurological disorders and allow the development of new tools for reconstructing damaged neural circuits.</p>}},
author = {{Palma-Tortosa, Sara and Martínez-Curiel, Raquel and Aretio-Medina, Constanza and Avaliani, Natalia and Kokaia, Zaal}},
issn = {{1940-087X}},
language = {{eng}},
number = {{190}},
publisher = {{JoVE}},
series = {{Journal of Visualized Experiments}},
title = {{Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation}},
url = {{http://dx.doi.org/10.3791/64234}},
doi = {{10.3791/64234}},
volume = {{2022}},
year = {{2022}},
}