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Heterotrophic nitrification and aerobic denitrification in Alcaligenes faecalis strain TUD

van Niel, Ed LU ; Braber, K.J.; Robertson, L.A. and Kuenen, J.G. (1992) In Antonie van Leeuwenhoek 62(3). p.231-237
Abstract
Heterotrophic nitrification and aerobic and anaerobic denitrification byAlcaligenes faecalis strain TUD were studied in continuous cultures under various environmental conditions. Both nitrification and denitrification activities increased with the dilution rate. At dissolved oxygen concentrations above 46% air saturation, hydroxylamine, nitrite and nitrate accumulated, indicating that both the nitrification and denitrification were less efficient. The overall nitrification activity was, however, essentially unaffected by the oxygen concentration. The nitrification rate increased with increasing ammonia concentration, but was lower in the presence of nitrate or nitrite. When present, hydroxylamine, was nitrified preferentially. Relatively... (More)
Heterotrophic nitrification and aerobic and anaerobic denitrification byAlcaligenes faecalis strain TUD were studied in continuous cultures under various environmental conditions. Both nitrification and denitrification activities increased with the dilution rate. At dissolved oxygen concentrations above 46% air saturation, hydroxylamine, nitrite and nitrate accumulated, indicating that both the nitrification and denitrification were less efficient. The overall nitrification activity was, however, essentially unaffected by the oxygen concentration. The nitrification rate increased with increasing ammonia concentration, but was lower in the presence of nitrate or nitrite. When present, hydroxylamine, was nitrified preferentially. Relatively low concentrations of acetate caused substrate inhibition (KI=109 μM acetate). Denitrifying or assimilatory nitrate reductases were not detected, and the copper nitrite reductase, rather than cytochrome cd, was present. Thiosulphate (a potential inhibitor of heterotrophic nitrification) was oxidized byA. faecalis strain TUD, with a maximum oxygen uptake rate of 140–170nmol O2·min-1·mg prot-1. Comparison of the behaviour ofA. faecalis TUD with that of other bacteria capable of heterotrophic nitrification and aerobic denitrification established that the response of these organisms to environmental parameters is not uniform. Similarities were found in their responses to dissolved oxygen concentrations, growth rate and ammonia concentration. However, they differed in their responses to externally supplied nitrite and nitrate. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
keywords
Alcaligenes faecalis, denitrification, heterotrophic nitification, chemostat
in
Antonie van Leeuwenhoek
volume
62
issue
3
pages
7 pages
publisher
Springer
external identifiers
  • scopus:0026686892
ISSN
0003-6072
language
English
LU publication?
no
id
cb28215a-2ff7-4933-be5c-f7f0f916a0f8
date added to LUP
2016-08-26 13:40:16
date last changed
2017-08-20 05:01:27
@article{cb28215a-2ff7-4933-be5c-f7f0f916a0f8,
  abstract     = {Heterotrophic nitrification and aerobic and anaerobic denitrification byAlcaligenes faecalis strain TUD were studied in continuous cultures under various environmental conditions. Both nitrification and denitrification activities increased with the dilution rate. At dissolved oxygen concentrations above 46% air saturation, hydroxylamine, nitrite and nitrate accumulated, indicating that both the nitrification and denitrification were less efficient. The overall nitrification activity was, however, essentially unaffected by the oxygen concentration. The nitrification rate increased with increasing ammonia concentration, but was lower in the presence of nitrate or nitrite. When present, hydroxylamine, was nitrified preferentially. Relatively low concentrations of acetate caused substrate inhibition (KI=109 μM acetate). Denitrifying or assimilatory nitrate reductases were not detected, and the copper nitrite reductase, rather than cytochrome cd, was present. Thiosulphate (a potential inhibitor of heterotrophic nitrification) was oxidized byA. faecalis strain TUD, with a maximum oxygen uptake rate of 140–170nmol O2·min-1·mg prot-1. Comparison of the behaviour ofA. faecalis TUD with that of other bacteria capable of heterotrophic nitrification and aerobic denitrification established that the response of these organisms to environmental parameters is not uniform. Similarities were found in their responses to dissolved oxygen concentrations, growth rate and ammonia concentration. However, they differed in their responses to externally supplied nitrite and nitrate.},
  author       = {van Niel, Ed and Braber, K.J. and Robertson, L.A. and Kuenen, J.G.},
  issn         = {0003-6072},
  keyword      = {Alcaligenes faecalis,denitrification,heterotrophic nitification,chemostat},
  language     = {eng},
  number       = {3},
  pages        = {231--237},
  publisher    = {Springer},
  series       = {Antonie van Leeuwenhoek},
  title        = {Heterotrophic nitrification and aerobic denitrification in Alcaligenes faecalis strain TUD},
  volume       = {62},
  year         = {1992},
}