Quantitative characterization of light-harvesting efficiency in single molecules and nanoparticles by 2D polarization microscopy: Experimental and theoretical challenges
Camacho Dejay, Rafael LU ; Thomsson, Daniel LU ; Yadav, Dheerendra LU and Scheblykin, Ivan LU
(2012)
In Chemical Physics
406.
p.30-40
- Abstract
- General problem of extracting intramolecular energy transfer information from fluorescence and fluorescence excitation polarization experiments at single molecule level is presented. A single funnel approximation is shown to be a very powerful approach to model the polarization data obtained by recently emerged 2-dimensional polarization single molecule imaging technique [O. Mirzov et al., Small 5 (2009) 1877]. Using this approximation a parameter characterising quantitatively light-harvesting efficiency of an individual light-harvesting antenna can be readily obtained. Technical details of 2D polarization imaging and practical methods of avoiding polarization artefact in fluorescence microscopy are discussed. (C) 2012 Elsevier B. V. All... (More)
- General problem of extracting intramolecular energy transfer information from fluorescence and fluorescence excitation polarization experiments at single molecule level is presented. A single funnel approximation is shown to be a very powerful approach to model the polarization data obtained by recently emerged 2-dimensional polarization single molecule imaging technique [O. Mirzov et al., Small 5 (2009) 1877]. Using this approximation a parameter characterising quantitatively light-harvesting efficiency of an individual light-harvesting antenna can be readily obtained. Technical details of 2D polarization imaging and practical methods of avoiding polarization artefact in fluorescence microscopy are discussed. (C) 2012 Elsevier B. V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3276237
- author
- Camacho Dejay, Rafael
LU
; Thomsson, Daniel
LU
; Yadav, Dheerendra
LU
and Scheblykin, Ivan
LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Light-harvesting, Antenna, Single molecule spectroscopy, Anisotropy, Fluorescence, FRET, Energy funnelling
- in
- Chemical Physics
- volume
- 406
- pages
- 30 - 40
- publisher
- Elsevier
- external identifiers
-
- wos:000310569800006
- scopus:84868207513
- ISSN
- 0301-0104
- DOI
- 10.1016/j.chemphys.2012.03.001
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Chemical Physics (S) (011001060)
- id
- cb37fa57-3389-4f11-8c52-0bb478f2061a (old id 3276237)
- date added to LUP
- 2016-04-01 13:02:52
- date last changed
- 2023-11-12 11:18:49
@article{cb37fa57-3389-4f11-8c52-0bb478f2061a,
abstract = {{General problem of extracting intramolecular energy transfer information from fluorescence and fluorescence excitation polarization experiments at single molecule level is presented. A single funnel approximation is shown to be a very powerful approach to model the polarization data obtained by recently emerged 2-dimensional polarization single molecule imaging technique [O. Mirzov et al., Small 5 (2009) 1877]. Using this approximation a parameter characterising quantitatively light-harvesting efficiency of an individual light-harvesting antenna can be readily obtained. Technical details of 2D polarization imaging and practical methods of avoiding polarization artefact in fluorescence microscopy are discussed. (C) 2012 Elsevier B. V. All rights reserved.}},
author = {{Camacho Dejay, Rafael and Thomsson, Daniel and Yadav, Dheerendra and Scheblykin, Ivan}},
issn = {{0301-0104}},
keywords = {{Light-harvesting; Antenna; Single molecule spectroscopy; Anisotropy; Fluorescence; FRET; Energy funnelling}},
language = {{eng}},
pages = {{30--40}},
publisher = {{Elsevier}},
series = {{Chemical Physics}},
title = {{Quantitative characterization of light-harvesting efficiency in single molecules and nanoparticles by 2D polarization microscopy: Experimental and theoretical challenges}},
url = {{http://dx.doi.org/10.1016/j.chemphys.2012.03.001}},
doi = {{10.1016/j.chemphys.2012.03.001}},
volume = {{406}},
year = {{2012}},
}