Synthesis of the disaccharide 6-O-β-d-galactopyranosyl-2-acetamido-2-deoxy-d-galactose using immobilized β-galactosidase
(1984) In Biochemical and Biophysical Research Communications 123(1). p.8-15- Abstract
- The disaccharide 6-O-β-d-galactopyranosyl-2-acetamido-2-deoxy-d-galactose has been synthesized by transfer of the β-d-galactopyranosyl residue from lactose to 2-acetamido-2-deoxy-d-galactose utilizing the transferase activity of β-galactosidase from
. To make the enzyme reusable, it was applied in an immobilized form covalently bound to Sepharose CL-4B. The yield of the disaccharide was about 20%, calculated on the amount of acetamido-deoxy-d-galactose added. The disaccharide could also be obtained by reversal of the hydrolytic activity of the enzyme, using d-galactose and 2-acetamido-2-deoxy-d-galactose as substrate. The yield in this reaction, however, was only 2–3 % under the conditions applied.
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https://lup.lub.lu.se/record/cb575e5c-df3a-4d38-975c-d581c033e775
- author
- Hedbys, Lars ; Larsson, Per-Olof LU ; Mosbach, Klaus LU and Svensson, Sigfrid
- organization
- publishing date
- 1984
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Disaccharide synthesis, E.coli beta-galactosidase, Immobilized enzyme, Transferase reaction, rReversed hydrolysis reaction
- in
- Biochemical and Biophysical Research Communications
- volume
- 123
- issue
- 1
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0021773804
- ISSN
- 0006-291X
- DOI
- 10.1016/0006-291X(84)90372-3
- language
- English
- LU publication?
- yes
- id
- cb575e5c-df3a-4d38-975c-d581c033e775
- date added to LUP
- 2024-06-18 15:11:36
- date last changed
- 2024-09-06 13:49:54
@article{cb575e5c-df3a-4d38-975c-d581c033e775, abstract = {{The disaccharide 6-O-β-d-galactopyranosyl-2-acetamido-2-deoxy-d-galactose has been synthesized by transfer of the β-d-galactopyranosyl residue from lactose to 2-acetamido-2-deoxy-d-galactose utilizing the transferase activity of β-galactosidase from <br/>. To make the enzyme reusable, it was applied in an immobilized form covalently bound to Sepharose CL-4B. The yield of the disaccharide was about 20%, calculated on the amount of acetamido-deoxy-d-galactose added. The disaccharide could also be obtained by reversal of the hydrolytic activity of the enzyme, using d-galactose and 2-acetamido-2-deoxy-d-galactose as substrate. The yield in this reaction, however, was only 2–3 % under the conditions applied.}}, author = {{Hedbys, Lars and Larsson, Per-Olof and Mosbach, Klaus and Svensson, Sigfrid}}, issn = {{0006-291X}}, keywords = {{Disaccharide synthesis; E.coli beta-galactosidase; Immobilized enzyme; Transferase reaction; rReversed hydrolysis reaction}}, language = {{eng}}, number = {{1}}, pages = {{8--15}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Synthesis of the disaccharide 6-<i>O</i>-β-d-galactopyranosyl-2-acetamido-2-deoxy-d-galactose using immobilized β-galactosidase}}, url = {{http://dx.doi.org/10.1016/0006-291X(84)90372-3}}, doi = {{10.1016/0006-291X(84)90372-3}}, volume = {{123}}, year = {{1984}}, }