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Synthesis of the disaccharide 6-O-β-d-galactopyranosyl-2-acetamido-2-deoxy-d-galactose using immobilized β-galactosidase

Hedbys, Lars ; Larsson, Per-Olof LU ; Mosbach, Klaus LU and Svensson, Sigfrid (1984) In Biochemical and Biophysical Research Communications 123(1). p.8-15
Abstract
The disaccharide 6-O-β-d-galactopyranosyl-2-acetamido-2-deoxy-d-galactose has been synthesized by transfer of the β-d-galactopyranosyl residue from lactose to 2-acetamido-2-deoxy-d-galactose utilizing the transferase activity of β-galactosidase from
. To make the enzyme reusable, it was applied in an immobilized form covalently bound to Sepharose CL-4B. The yield of the disaccharide was about 20%, calculated on the amount of acetamido-deoxy-d-galactose added. The disaccharide could also be obtained by reversal of the hydrolytic activity of the enzyme, using d-galactose and 2-acetamido-2-deoxy-d-galactose as substrate. The yield in this reaction, however, was only 2–3 % under the conditions applied.
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Disaccharide synthesis, E.coli beta-galactosidase, Immobilized enzyme, Transferase reaction, rReversed hydrolysis reaction
in
Biochemical and Biophysical Research Communications
volume
123
issue
1
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:0021773804
ISSN
0006-291X
DOI
10.1016/0006-291X(84)90372-3
language
English
LU publication?
yes
id
cb575e5c-df3a-4d38-975c-d581c033e775
date added to LUP
2024-06-18 15:11:36
date last changed
2025-10-14 09:28:15
@article{cb575e5c-df3a-4d38-975c-d581c033e775,
  abstract     = {{The disaccharide 6-O-β-d-galactopyranosyl-2-acetamido-2-deoxy-d-galactose has been synthesized by transfer of the β-d-galactopyranosyl residue from lactose to 2-acetamido-2-deoxy-d-galactose utilizing the transferase activity of β-galactosidase from <br/>. To make the enzyme reusable, it was applied in an immobilized form covalently bound to Sepharose CL-4B. The yield of the disaccharide was about 20%, calculated on the amount of acetamido-deoxy-d-galactose added. The disaccharide could also be obtained by reversal of the hydrolytic activity of the enzyme, using d-galactose and 2-acetamido-2-deoxy-d-galactose as substrate. The yield in this reaction, however, was only 2–3 % under the conditions applied.}},
  author       = {{Hedbys, Lars and Larsson, Per-Olof and Mosbach, Klaus and Svensson, Sigfrid}},
  issn         = {{0006-291X}},
  keywords     = {{Disaccharide synthesis; E.coli beta-galactosidase; Immobilized enzyme; Transferase reaction; rReversed hydrolysis reaction}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{8--15}},
  publisher    = {{Elsevier}},
  series       = {{Biochemical and Biophysical Research Communications}},
  title        = {{Synthesis of the disaccharide 6-<i>O</i>-β-d-galactopyranosyl-2-acetamido-2-deoxy-d-galactose using immobilized β-galactosidase}},
  url          = {{http://dx.doi.org/10.1016/0006-291X(84)90372-3}},
  doi          = {{10.1016/0006-291X(84)90372-3}},
  volume       = {{123}},
  year         = {{1984}},
}