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Cubosomes for in vivo fluorescence lifetime imaging

Biffi, Stefania; Andolfi, Laura; Caltagirone, Claudia; Garrovo, Chiara; Falchi, Angela M; Lippolis, Vito; Lorenzon, Andrea; Macor, Paolo; Meli, Valeria and Monduzzi, Maura, et al. (2017) In Nanotechnology 28(5).
Abstract

Herein we provided the first proof of principle for in vivo fluorescence optical imaging application using monoolein-based cubosomes in a healthy mouse animal model. This formulation, administered at a non-cytotoxic concentration, was capable of providing both exogenous contrast for NIR fluorescence imaging with very high efficiency and chemospecific information upon lifetime analysis. Time-resolved measurements of fluorescence after the intravenous injection of cubosomes revealed that the dye rapidly accumulated mainly in the liver, while lifetimes profiles obtained in vivo allowed for discriminating between free dye or dye embedded within the cubosome nanostructure after injection.

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type
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publication status
published
subject
keywords
fluorescence lifetime probe, fluorescence optical imaging in vivo, liquid crystalline nanoparticles
in
Nanotechnology
volume
28
issue
5
publisher
IOP Publishing
external identifiers
  • scopus:85009113821
  • wos:000412980600001
ISSN
0957-4484
DOI
10.1088/1361-6528/28/5/055102
language
English
LU publication?
yes
id
cb750a80-dd2a-4739-a7f2-7c2852079d8a
date added to LUP
2017-02-03 11:13:57
date last changed
2018-01-16 13:23:11
@article{cb750a80-dd2a-4739-a7f2-7c2852079d8a,
  abstract     = {<p>Herein we provided the first proof of principle for in vivo fluorescence optical imaging application using monoolein-based cubosomes in a healthy mouse animal model. This formulation, administered at a non-cytotoxic concentration, was capable of providing both exogenous contrast for NIR fluorescence imaging with very high efficiency and chemospecific information upon lifetime analysis. Time-resolved measurements of fluorescence after the intravenous injection of cubosomes revealed that the dye rapidly accumulated mainly in the liver, while lifetimes profiles obtained in vivo allowed for discriminating between free dye or dye embedded within the cubosome nanostructure after injection.</p>},
  articleno    = {055102},
  author       = {Biffi, Stefania and Andolfi, Laura and Caltagirone, Claudia and Garrovo, Chiara and Falchi, Angela M and Lippolis, Vito and Lorenzon, Andrea and Macor, Paolo and Meli, Valeria and Monduzzi, Maura and Obiols-Rabasa, Marc and Petrizza, Luca and Prodi, Luca and Rosa, Antonella and Schmidt, Judith and Talmon, Yeshayahu and Murgia, Sergio},
  issn         = {0957-4484},
  keyword      = {fluorescence lifetime probe,fluorescence optical imaging in vivo,liquid crystalline nanoparticles},
  language     = {eng},
  month        = {02},
  number       = {5},
  publisher    = {IOP Publishing},
  series       = {Nanotechnology},
  title        = {Cubosomes for in vivo fluorescence lifetime imaging},
  url          = {http://dx.doi.org/10.1088/1361-6528/28/5/055102},
  volume       = {28},
  year         = {2017},
}