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Increasing amperometric biosensor sensitivity by length fractionated single-walled carbon nanotubes.

Tasca, Federico LU ; Gorton, Lo LU ; Wagner, Jakob LU and Nöll, Gilbert LU (2008) In Biosensors & Bioelectronics 24(2). p.272-278
Abstract
In this work the sensitivity-increasing effect of single-walled carbon nanotubes (SWCNTs) in amperometric biosensors, depending on their average length distribution, was studied. For this purpose the SWCNTs were oxidatively shortened and subsequently length separated by size exclusion chromatography. Transmission electron micrographs of different fractions of SWCNTs were collected. Diaphorase "wired" to an osmium redox polymer was blended with the shortened SWCNTs of different lengths. Depending on the average length of the SWCNTs the sensitivity of the amperometric biosensor model system towards oxidation of 1,4-dihydronicotinamide adenine dinucleotide (NADH) was increased by a factor of five. The best performance was achieved with SWCNTs... (More)
In this work the sensitivity-increasing effect of single-walled carbon nanotubes (SWCNTs) in amperometric biosensors, depending on their average length distribution, was studied. For this purpose the SWCNTs were oxidatively shortened and subsequently length separated by size exclusion chromatography. Transmission electron micrographs of different fractions of SWCNTs were collected. Diaphorase "wired" to an osmium redox polymer was blended with the shortened SWCNTs of different lengths. Depending on the average length of the SWCNTs the sensitivity of the amperometric biosensor model system towards oxidation of 1,4-dihydronicotinamide adenine dinucleotide (NADH) was increased by a factor of five. The best performance was achieved with SWCNTs of medium length. The linear range for NADH detection was between 5muM and 7mM, the maximum sensitivity was 47nAmuM(-1)cm(-2), and the detection limit was 1muM. The biosensor exhibited excellent electrocatalytic properties. Even at relatively high NADH concentrations the oxidative current was limited by the diffusion rate of NADH. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amperometric biosensor, Diaphorase, NADH oxidation, Osmium redox polymer hydrogel, Length-separated single-walled carbon nanotubes (SWCNTs), Transmission electron microscopy (TEM)
in
Biosensors & Bioelectronics
volume
24
issue
2
pages
272 - 278
publisher
Elsevier
external identifiers
  • wos:000259711900017
  • pmid:18479907
  • scopus:48149115855
  • pmid:18479907
ISSN
1873-4235
DOI
10.1016/j.bios.2008.03.038
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Polymer and Materials Chemistry (LTH) (011001041)
id
cb8b0312-bb85-4386-96a5-4fc108374fd9 (old id 1154137)
date added to LUP
2016-04-01 13:19:18
date last changed
2020-01-12 11:54:07
@article{cb8b0312-bb85-4386-96a5-4fc108374fd9,
  abstract     = {In this work the sensitivity-increasing effect of single-walled carbon nanotubes (SWCNTs) in amperometric biosensors, depending on their average length distribution, was studied. For this purpose the SWCNTs were oxidatively shortened and subsequently length separated by size exclusion chromatography. Transmission electron micrographs of different fractions of SWCNTs were collected. Diaphorase "wired" to an osmium redox polymer was blended with the shortened SWCNTs of different lengths. Depending on the average length of the SWCNTs the sensitivity of the amperometric biosensor model system towards oxidation of 1,4-dihydronicotinamide adenine dinucleotide (NADH) was increased by a factor of five. The best performance was achieved with SWCNTs of medium length. The linear range for NADH detection was between 5muM and 7mM, the maximum sensitivity was 47nAmuM(-1)cm(-2), and the detection limit was 1muM. The biosensor exhibited excellent electrocatalytic properties. Even at relatively high NADH concentrations the oxidative current was limited by the diffusion rate of NADH.},
  author       = {Tasca, Federico and Gorton, Lo and Wagner, Jakob and Nöll, Gilbert},
  issn         = {1873-4235},
  language     = {eng},
  number       = {2},
  pages        = {272--278},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Increasing amperometric biosensor sensitivity by length fractionated single-walled carbon nanotubes.},
  url          = {http://dx.doi.org/10.1016/j.bios.2008.03.038},
  doi          = {10.1016/j.bios.2008.03.038},
  volume       = {24},
  year         = {2008},
}