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Active and separate secretion of fiber and penton base during the early phase of Ad2 or Ad5 infection

Yan, Yuhua; Zhang, Bo; Hou, Weihong; Lin, Hongyu; Rebetz, Johan LU ; Hong, Saw See; Wang, Youjun; Ran, Liang and Fan, Xiaolong LU (2017) In Virology 505. p.172-180
Abstract

Fiber and penton base overproduced in adenovirus (Ad) infected cells can be secreted prior to progeny release and thereby regulate progeny spread. We aimed to investigate the mechanisms of fiber and penton base secretion in Ad2- or Ad5-infected A549 cells. Our flow cytometry analyses detected abundant surface fiber molecules, but little penton base molecules at 12 h post infection. Immunogold staining combined with transmission electron microscopic analyses revealed separate, non-co-localized release of fiber and penton base in the proximity of the plasma membrane. Depolymerization of microtubule and actin cytoskeletons, and inhibition of Rock kinase and myosin II activity together demonstrated cytoskeletal network-dependent fiber... (More)

Fiber and penton base overproduced in adenovirus (Ad) infected cells can be secreted prior to progeny release and thereby regulate progeny spread. We aimed to investigate the mechanisms of fiber and penton base secretion in Ad2- or Ad5-infected A549 cells. Our flow cytometry analyses detected abundant surface fiber molecules, but little penton base molecules at 12 h post infection. Immunogold staining combined with transmission electron microscopic analyses revealed separate, non-co-localized release of fiber and penton base in the proximity of the plasma membrane. Depolymerization of microtubule and actin cytoskeletons, and inhibition of Rock kinase and myosin II activity together demonstrated cytoskeletal network-dependent fiber secretion. Inhibition of intracellular calcium [Ca2+]i signaling caused diminished fiber secretion, which was associated with diminished progeny production. Thus, fiber and penton base are actively and separately secreted during the early stages of Ad2 or Ad5 infection, their secretion may play important role in Ad life cycle.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adenovirus, Fiber protein, Penton base, Secretion
in
Virology
volume
505
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:85014250662
  • wos:000398650400017
ISSN
0042-6822
DOI
10.1016/j.virol.2017.02.018
language
English
LU publication?
yes
id
cb959486-0778-4d00-9846-71a99c3b150b
date added to LUP
2017-03-14 11:01:09
date last changed
2018-01-07 11:55:06
@article{cb959486-0778-4d00-9846-71a99c3b150b,
  abstract     = {<p>Fiber and penton base overproduced in adenovirus (Ad) infected cells can be secreted prior to progeny release and thereby regulate progeny spread. We aimed to investigate the mechanisms of fiber and penton base secretion in Ad2- or Ad5-infected A549 cells. Our flow cytometry analyses detected abundant surface fiber molecules, but little penton base molecules at 12 h post infection. Immunogold staining combined with transmission electron microscopic analyses revealed separate, non-co-localized release of fiber and penton base in the proximity of the plasma membrane. Depolymerization of microtubule and actin cytoskeletons, and inhibition of Rock kinase and myosin II activity together demonstrated cytoskeletal network-dependent fiber secretion. Inhibition of intracellular calcium [Ca<sup>2+</sup>]<sub>i</sub> signaling caused diminished fiber secretion, which was associated with diminished progeny production. Thus, fiber and penton base are actively and separately secreted during the early stages of Ad2 or Ad5 infection, their secretion may play important role in Ad life cycle.</p>},
  author       = {Yan, Yuhua and Zhang, Bo and Hou, Weihong and Lin, Hongyu and Rebetz, Johan and Hong, Saw See and Wang, Youjun and Ran, Liang and Fan, Xiaolong},
  issn         = {0042-6822},
  keyword      = {Adenovirus,Fiber protein,Penton base,Secretion},
  language     = {eng},
  month        = {05},
  pages        = {172--180},
  publisher    = {Elsevier},
  series       = {Virology},
  title        = {Active and separate secretion of fiber and penton base during the early phase of Ad2 or Ad5 infection},
  url          = {http://dx.doi.org/10.1016/j.virol.2017.02.018},
  volume       = {505},
  year         = {2017},
}