Active and separate secretion of fiber and penton base during the early phase of Ad2 or Ad5 infection
Yan, Yuhua ; Zhang, Bo ; Hou, Weihong ; Lin, Hongyu ; Rebetz, Johan LU
; Hong, Saw See
; Wang, Youjun
; Ran, Liang
and Fan, Xiaolong
LU
(2017)
In Virology
505.
p.172-180
- Abstract
Fiber and penton base overproduced in adenovirus (Ad) infected cells can be secreted prior to progeny release and thereby regulate progeny spread. We aimed to investigate the mechanisms of fiber and penton base secretion in Ad2- or Ad5-infected A549 cells. Our flow cytometry analyses detected abundant surface fiber molecules, but little penton base molecules at 12 h post infection. Immunogold staining combined with transmission electron microscopic analyses revealed separate, non-co-localized release of fiber and penton base in the proximity of the plasma membrane. Depolymerization of microtubule and actin cytoskeletons, and inhibition of Rock kinase and myosin II activity together demonstrated cytoskeletal network-dependent fiber... (More)
Fiber and penton base overproduced in adenovirus (Ad) infected cells can be secreted prior to progeny release and thereby regulate progeny spread. We aimed to investigate the mechanisms of fiber and penton base secretion in Ad2- or Ad5-infected A549 cells. Our flow cytometry analyses detected abundant surface fiber molecules, but little penton base molecules at 12 h post infection. Immunogold staining combined with transmission electron microscopic analyses revealed separate, non-co-localized release of fiber and penton base in the proximity of the plasma membrane. Depolymerization of microtubule and actin cytoskeletons, and inhibition of Rock kinase and myosin II activity together demonstrated cytoskeletal network-dependent fiber secretion. Inhibition of intracellular calcium [Ca2+]i signaling caused diminished fiber secretion, which was associated with diminished progeny production. Thus, fiber and penton base are actively and separately secreted during the early stages of Ad2 or Ad5 infection, their secretion may play important role in Ad life cycle.
(Less)
- author
- Yan, Yuhua
; Zhang, Bo
; Hou, Weihong
; Lin, Hongyu
; Rebetz, Johan
LU
; Hong, Saw See
; Wang, Youjun
; Ran, Liang
and Fan, Xiaolong
LU
- organization
- publishing date
- 2017-05-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adenovirus, Fiber protein, Penton base, Secretion
- in
- Virology
- volume
- 505
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85014250662
- pmid:28264780
- wos:000398650400017
- ISSN
- 0042-6822
- DOI
- 10.1016/j.virol.2017.02.018
- language
- English
- LU publication?
- yes
- id
- cb959486-0778-4d00-9846-71a99c3b150b
- date added to LUP
- 2017-03-14 11:01:09
- date last changed
- 2024-05-26 11:57:07
@article{cb959486-0778-4d00-9846-71a99c3b150b,
abstract = {{<p>Fiber and penton base overproduced in adenovirus (Ad) infected cells can be secreted prior to progeny release and thereby regulate progeny spread. We aimed to investigate the mechanisms of fiber and penton base secretion in Ad2- or Ad5-infected A549 cells. Our flow cytometry analyses detected abundant surface fiber molecules, but little penton base molecules at 12 h post infection. Immunogold staining combined with transmission electron microscopic analyses revealed separate, non-co-localized release of fiber and penton base in the proximity of the plasma membrane. Depolymerization of microtubule and actin cytoskeletons, and inhibition of Rock kinase and myosin II activity together demonstrated cytoskeletal network-dependent fiber secretion. Inhibition of intracellular calcium [Ca<sup>2+</sup>]<sub>i</sub> signaling caused diminished fiber secretion, which was associated with diminished progeny production. Thus, fiber and penton base are actively and separately secreted during the early stages of Ad2 or Ad5 infection, their secretion may play important role in Ad life cycle.</p>}},
author = {{Yan, Yuhua and Zhang, Bo and Hou, Weihong and Lin, Hongyu and Rebetz, Johan and Hong, Saw See and Wang, Youjun and Ran, Liang and Fan, Xiaolong}},
issn = {{0042-6822}},
keywords = {{Adenovirus; Fiber protein; Penton base; Secretion}},
language = {{eng}},
month = {{05}},
pages = {{172--180}},
publisher = {{Elsevier}},
series = {{Virology}},
title = {{Active and separate secretion of fiber and penton base during the early phase of Ad2 or Ad5 infection}},
url = {{http://dx.doi.org/10.1016/j.virol.2017.02.018}},
doi = {{10.1016/j.virol.2017.02.018}},
volume = {{505}},
year = {{2017}},
}