Preparation of stable radioiodinated polypeptide hormones and proteins using polyacrylamide gel electrophoresis
Linde, Susanne ; Hansen, Bruno and Lernmark, Åke LU
(1983)
In Methods in enzymology
92.
p.309-335
- Abstract
- This chapter discusses the preparation of stable radioiodinated polypeptide hormones and proteins using polyacrylamide gel electrophoresis. Radioactively labeled polypeptide hormones and proteins are widely used as tracers in radioimmunoassays and receptor studies. The peptide or protein is most easily labeled using iodination with 125I or 131I. The radioactive iodine is substituted in the tyrosine groups of the peptide as monoiodotyrosine or di-iodotyrosine, resulting in a heterogeneous mixture of iodine-substituted molecules plus native peptide and unreacted iodide. The iodination mixture has to be fractionated to obtain a well-characterized iodinated product to be used as a tracer. An ideal tracer should retain the full biological... (More)
- This chapter discusses the preparation of stable radioiodinated polypeptide hormones and proteins using polyacrylamide gel electrophoresis. Radioactively labeled polypeptide hormones and proteins are widely used as tracers in radioimmunoassays and receptor studies. The peptide or protein is most easily labeled using iodination with 125I or 131I. The radioactive iodine is substituted in the tyrosine groups of the peptide as monoiodotyrosine or di-iodotyrosine, resulting in a heterogeneous mixture of iodine-substituted molecules plus native peptide and unreacted iodide. The iodination mixture has to be fractionated to obtain a well-characterized iodinated product to be used as a tracer. An ideal tracer should retain the full biological activity or immunological reactivity of the native peptide hormone or protein and have a high specific activity and a long shelf life. Studies in the laboratory have shown that it is possible to fractionate iodinated insulin preparations into homogeneous monoiodoinsulin derivatives by polyacrylamide gel electrophoresis using long rods. The same fractionation technique has been useful for the preparation of a number of radioiodinated polypeptide hormones and proteins. (Less)
Please use this url to cite or link to this publication:
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- author
- Linde, Susanne
; Hansen, Bruno
and Lernmark, Åke
LU
- publishing date
- 1983
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- Immunochemical Techniques Part E : Monoclonal Antibodies and General Immunoassay Methods - Monoclonal Antibodies and General Immunoassay Methods
- series title
- Methods in enzymology
- editor
- Langone, John J ; Van Vunakis, Helen ; Colowick, Sidney P and Kaplan, Nathan O
- volume
- 92
- pages
- 309 - 335
- publisher
- Academic Press
- ISBN
- 0-12-181992-2
- language
- English
- LU publication?
- no
- id
- cbee49fe-10fd-447e-8764-e8123b0d286f
- date added to LUP
- 2021-09-23 10:55:33
- date last changed
- 2021-09-24 04:01:21
@inbook{cbee49fe-10fd-447e-8764-e8123b0d286f,
abstract = {{This chapter discusses the preparation of stable radioiodinated polypeptide hormones and proteins using polyacrylamide gel electrophoresis. Radioactively labeled polypeptide hormones and proteins are widely used as tracers in radioimmunoassays and receptor studies. The peptide or protein is most easily labeled using iodination with 125I or 131I. The radioactive iodine is substituted in the tyrosine groups of the peptide as monoiodotyrosine or di-iodotyrosine, resulting in a heterogeneous mixture of iodine-substituted molecules plus native peptide and unreacted iodide. The iodination mixture has to be fractionated to obtain a well-characterized iodinated product to be used as a tracer. An ideal tracer should retain the full biological activity or immunological reactivity of the native peptide hormone or protein and have a high specific activity and a long shelf life. Studies in the laboratory have shown that it is possible to fractionate iodinated insulin preparations into homogeneous monoiodoinsulin derivatives by polyacrylamide gel electrophoresis using long rods. The same fractionation technique has been useful for the preparation of a number of radioiodinated polypeptide hormones and proteins.}},
author = {{Linde, Susanne and Hansen, Bruno and Lernmark, Åke}},
booktitle = {{Immunochemical Techniques Part E : Monoclonal Antibodies and General Immunoassay Methods}},
editor = {{Langone, John J and Van Vunakis, Helen and Colowick, Sidney P and Kaplan, Nathan O}},
isbn = {{0-12-181992-2}},
language = {{eng}},
pages = {{309--335}},
publisher = {{Academic Press}},
series = {{Methods in enzymology}},
title = {{Preparation of stable radioiodinated polypeptide hormones and proteins using polyacrylamide gel electrophoresis}},
volume = {{92}},
year = {{1983}},
}