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Stable Homogeneous Gel for Molecular-Sieving of DNA Fragments in Capillary Electrophoresis

Végvári, Ákos LU and Hjertén, Stellan (2002) In Journal of Chromatography A 960(1-2). p.221-227
Abstract
A polyacrylamide gel crosslinked with allyl-b-cyclodextrin can be used repeatedly for several weeks for the separation of DNA fragments, since bubbles are not generated during a run. Allyl-b-cyclodextrin can easily be synthesized in one step from allylglycidylether and b-cyclodextrin. The plate numbers for DNA fragments, up to about 1500 bp, are high: for the 21 separation of pBR322/HaeIII fragments they were in the range 450 000–1 600 000 m . The resolution was almost independent of the concentration of the crosslinker (allyl-b-cyclodextrin) — in sharp contrast to gels crosslinked with N,N9-methylenebisacrylamide.
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author
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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Capillary electrophoresis, DNA, Polyacrylamide, Allyl-b-cyclodextrin
in
Journal of Chromatography A
volume
960
issue
1-2
pages
221 - 227
publisher
Elsevier
external identifiers
  • scopus:0037172696
ISSN
0021-9673
DOI
10.1016/S0021-9673(01)01436-4,
language
English
LU publication?
no
id
cc510027-4876-49d2-a341-fdf6e796c72d (old id 3516236)
date added to LUP
2016-04-04 09:17:51
date last changed
2025-10-14 13:06:19
@article{cc510027-4876-49d2-a341-fdf6e796c72d,
  abstract     = {{A polyacrylamide gel crosslinked with allyl-b-cyclodextrin can be used repeatedly for several weeks for the separation of DNA fragments, since bubbles are not generated during a run. Allyl-b-cyclodextrin can easily be synthesized in one step from allylglycidylether and b-cyclodextrin. The plate numbers for DNA fragments, up to about 1500 bp, are high: for the 21 separation of pBR322/HaeIII fragments they were in the range 450 000–1 600 000 m . The resolution was almost independent of the concentration of the crosslinker (allyl-b-cyclodextrin) — in sharp contrast to gels crosslinked with N,N9-methylenebisacrylamide.}},
  author       = {{Végvári, Ákos and Hjertén, Stellan}},
  issn         = {{0021-9673}},
  keywords     = {{Capillary electrophoresis; DNA; Polyacrylamide; Allyl-b-cyclodextrin}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{221--227}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography A}},
  title        = {{Stable Homogeneous Gel for Molecular-Sieving of DNA Fragments in Capillary Electrophoresis}},
  url          = {{http://dx.doi.org/10.1016/S0021-9673(01)01436-4,}},
  doi          = {{10.1016/S0021-9673(01)01436-4,}},
  volume       = {{960}},
  year         = {{2002}},
}