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Bradykinin sequesters B2 bradykinin receptors and the receptor-coupled Ga SUBUNITS Gaq and Gai in caveolae

De Weerd, W. F C and Leeb-Lundberg, L. M F LU (1997) In FASEB Journal 11(9).
Abstract

Caveolae are small flask-like invaginations on the plasma membrane that contain both G-proteins aim G-protein coupled receptors. Consequently, these structures have been implicated to serve a role in transmembrane signaling. Bradykinin (BK) is a potent vasoactive peptide that acts through B2 BK receptors to stimulate both Gaq and Gai. DDT1 MF2 smooth muscle cells were used to show that eaveolae contain both Gaq, Gai and B2 receptors. Ga subunits and caveolin, a marker protein for eaveolae, were monitored by iminunoblotting, while B2 receptors were monitored as receptor radioligand complexes which were formed prior to cell disruption and caveolae enrichment. At 4°C, both agonist and antagonist receptor complexes were detected in... (More)

Caveolae are small flask-like invaginations on the plasma membrane that contain both G-proteins aim G-protein coupled receptors. Consequently, these structures have been implicated to serve a role in transmembrane signaling. Bradykinin (BK) is a potent vasoactive peptide that acts through B2 BK receptors to stimulate both Gaq and Gai. DDT1 MF2 smooth muscle cells were used to show that eaveolae contain both Gaq, Gai and B2 receptors. Ga subunits and caveolin, a marker protein for eaveolae, were monitored by iminunoblotting, while B2 receptors were monitored as receptor radioligand complexes which were formed prior to cell disruption and caveolae enrichment. At 4°C, both agonist and antagonist receptor complexes were detected in eaveolae, and both types of complexes were acid-sensitive. Elevation of the temperature to 37°C resulted in a recruitment of agonist receptor complexes to caveotae and an increased acid-resistance of these complexes. Furthermore, these con ditions also promoted the recruitment of Gaq and Gci subunits. In contrast, antagonist receptor complexes were not recruited and remained acid-sensitive, and antagonists did not recruit Ga subunits. These results show that agonists for some G-protein-coupled receptors sequester their receptors and receptorcoupled Ga subunits in caveolae.

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publication status
published
subject
in
FASEB Journal
volume
11
issue
9
publisher
Wiley
external identifiers
  • scopus:33750253667
ISSN
0892-6638
language
English
LU publication?
no
id
cc546473-c3b8-47f9-bfa6-98d7f7c67898
date added to LUP
2019-06-10 11:10:03
date last changed
2023-09-09 05:33:13
@article{cc546473-c3b8-47f9-bfa6-98d7f7c67898,
  abstract     = {{<p>Caveolae are small flask-like invaginations on the plasma membrane that contain both G-proteins aim G-protein coupled receptors. Consequently, these structures have been implicated to serve a role in transmembrane signaling. Bradykinin (BK) is a potent vasoactive peptide that acts through B2 BK receptors to stimulate both Gaq and Gai. DDT1 MF2 smooth muscle cells were used to show that eaveolae contain both Gaq, Gai and B2 receptors. Ga subunits and caveolin, a marker protein for eaveolae, were monitored by iminunoblotting, while B2 receptors were monitored as receptor radioligand complexes which were formed prior to cell disruption and caveolae enrichment. At 4°C, both agonist and antagonist receptor complexes were detected in eaveolae, and both types of complexes were acid-sensitive. Elevation of the temperature to 37°C resulted in a recruitment of agonist receptor complexes to caveotae and an increased acid-resistance of these complexes. Furthermore, these con ditions also promoted the recruitment of Gaq and Gci subunits. In contrast, antagonist receptor complexes were not recruited and remained acid-sensitive, and antagonists did not recruit Ga subunits. These results show that agonists for some G-protein-coupled receptors sequester their receptors and receptorcoupled Ga subunits in caveolae.</p>}},
  author       = {{De Weerd, W. F C and Leeb-Lundberg, L. M F}},
  issn         = {{0892-6638}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{9}},
  publisher    = {{Wiley}},
  series       = {{FASEB Journal}},
  title        = {{Bradykinin sequesters B2 bradykinin receptors and the receptor-coupled Ga SUBUNITS Gaq and Gai in caveolae}},
  volume       = {{11}},
  year         = {{1997}},
}