Detection and purification of rat and goat immunoglobulin G antibodies using protein G-based solid-phase radioimmunoassays
Nilson, B LU
; Björck, L
LU
and Akerström, B
LU
(1986)
In Journal of Immunological Methods
91(2).
p.81-275
- Abstract
Using the newly described streptococcal surface protein, protein G, which has powerful immunoglobulin G binding properties, solid-phase radioimmunoassays were developed for the quantitation of goat and rat immunoglobulin G bound to the plastic surface of microtiter plates. The binding of goat immunoglobulin G to the surface via a specific antigen (guinea pig alpha 1-microglobulin) permitted the determination of antigen-specific antibodies with a detection limit of 50-100 ng. Optimum assay conditions were established and the whole assay procedure could be brought to completion at room temperature in less than a working day. The value of the assays was illustrated by monitoring rat and goat immunoglobulin G antibodies during their... (More)
Using the newly described streptococcal surface protein, protein G, which has powerful immunoglobulin G binding properties, solid-phase radioimmunoassays were developed for the quantitation of goat and rat immunoglobulin G bound to the plastic surface of microtiter plates. The binding of goat immunoglobulin G to the surface via a specific antigen (guinea pig alpha 1-microglobulin) permitted the determination of antigen-specific antibodies with a detection limit of 50-100 ng. Optimum assay conditions were established and the whole assay procedure could be brought to completion at room temperature in less than a working day. The value of the assays was illustrated by monitoring rat and goat immunoglobulin G antibodies during their purification from whole sera by classical chromatographic procedures.
(Less)
- author
- Nilson, B
LU
; Björck, L
LU
and Akerström, B
LU
- organization
- publishing date
- 1986-07-24
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Alpha-Globulins, Animals, Antibodies, Goats, Immunoglobulin G, Immunosorbent Techniques, Nerve Tissue Proteins, Radioimmunoassay, Rats, Temperature, Journal Article, Research Support, Non-U.S. Gov't
- in
- Journal of Immunological Methods
- volume
- 91
- issue
- 2
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:2426365
- scopus:0022535330
- ISSN
- 0022-1759
- language
- English
- LU publication?
- yes
- id
- cc95ec27-7a59-42eb-8dcc-872dd20f6628
- date added to LUP
- 2018-05-26 14:11:57
- date last changed
- 2024-01-14 20:55:59
@article{cc95ec27-7a59-42eb-8dcc-872dd20f6628,
abstract = {{<p>Using the newly described streptococcal surface protein, protein G, which has powerful immunoglobulin G binding properties, solid-phase radioimmunoassays were developed for the quantitation of goat and rat immunoglobulin G bound to the plastic surface of microtiter plates. The binding of goat immunoglobulin G to the surface via a specific antigen (guinea pig alpha 1-microglobulin) permitted the determination of antigen-specific antibodies with a detection limit of 50-100 ng. Optimum assay conditions were established and the whole assay procedure could be brought to completion at room temperature in less than a working day. The value of the assays was illustrated by monitoring rat and goat immunoglobulin G antibodies during their purification from whole sera by classical chromatographic procedures.</p>}},
author = {{Nilson, B and Björck, L and Akerström, B}},
issn = {{0022-1759}},
keywords = {{Alpha-Globulins; Animals; Antibodies; Goats; Immunoglobulin G; Immunosorbent Techniques; Nerve Tissue Proteins; Radioimmunoassay; Rats; Temperature; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
month = {{07}},
number = {{2}},
pages = {{81--275}},
publisher = {{Elsevier}},
series = {{Journal of Immunological Methods}},
title = {{Detection and purification of rat and goat immunoglobulin G antibodies using protein G-based solid-phase radioimmunoassays}},
volume = {{91}},
year = {{1986}},
}