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Detection and purification of rat and goat immunoglobulin G antibodies using protein G-based solid-phase radioimmunoassays

Nilson, B LU ; Björck, L LU and Akerström, B (1986) In Journal of Immunological Methods 91(2). p.81-275
Abstract

Using the newly described streptococcal surface protein, protein G, which has powerful immunoglobulin G binding properties, solid-phase radioimmunoassays were developed for the quantitation of goat and rat immunoglobulin G bound to the plastic surface of microtiter plates. The binding of goat immunoglobulin G to the surface via a specific antigen (guinea pig alpha 1-microglobulin) permitted the determination of antigen-specific antibodies with a detection limit of 50-100 ng. Optimum assay conditions were established and the whole assay procedure could be brought to completion at room temperature in less than a working day. The value of the assays was illustrated by monitoring rat and goat immunoglobulin G antibodies during their... (More)

Using the newly described streptococcal surface protein, protein G, which has powerful immunoglobulin G binding properties, solid-phase radioimmunoassays were developed for the quantitation of goat and rat immunoglobulin G bound to the plastic surface of microtiter plates. The binding of goat immunoglobulin G to the surface via a specific antigen (guinea pig alpha 1-microglobulin) permitted the determination of antigen-specific antibodies with a detection limit of 50-100 ng. Optimum assay conditions were established and the whole assay procedure could be brought to completion at room temperature in less than a working day. The value of the assays was illustrated by monitoring rat and goat immunoglobulin G antibodies during their purification from whole sera by classical chromatographic procedures.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Alpha-Globulins, Animals, Antibodies, Goats, Immunoglobulin G, Immunosorbent Techniques, Nerve Tissue Proteins, Radioimmunoassay, Rats, Temperature, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Immunological Methods
volume
91
issue
2
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:0022535330
ISSN
0022-1759
language
English
LU publication?
yes
id
cc95ec27-7a59-42eb-8dcc-872dd20f6628
date added to LUP
2018-05-26 14:11:57
date last changed
2018-06-03 05:07:02
@article{cc95ec27-7a59-42eb-8dcc-872dd20f6628,
  abstract     = {<p>Using the newly described streptococcal surface protein, protein G, which has powerful immunoglobulin G binding properties, solid-phase radioimmunoassays were developed for the quantitation of goat and rat immunoglobulin G bound to the plastic surface of microtiter plates. The binding of goat immunoglobulin G to the surface via a specific antigen (guinea pig alpha 1-microglobulin) permitted the determination of antigen-specific antibodies with a detection limit of 50-100 ng. Optimum assay conditions were established and the whole assay procedure could be brought to completion at room temperature in less than a working day. The value of the assays was illustrated by monitoring rat and goat immunoglobulin G antibodies during their purification from whole sera by classical chromatographic procedures.</p>},
  author       = {Nilson, B and Björck, L and Akerström, B},
  issn         = {0022-1759},
  keyword      = {Alpha-Globulins,Animals,Antibodies,Goats,Immunoglobulin G,Immunosorbent Techniques,Nerve Tissue Proteins,Radioimmunoassay,Rats,Temperature,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {07},
  number       = {2},
  pages        = {81--275},
  publisher    = {Elsevier},
  series       = {Journal of Immunological Methods},
  title        = {Detection and purification of rat and goat immunoglobulin G antibodies using protein G-based solid-phase radioimmunoassays},
  volume       = {91},
  year         = {1986},
}