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CX3C chemokine receptor 1 deficiency modulates microglia morphology but does not affect lesion size and short-term deficits after experimental stroke

van der Maten, Gerlinde LU ; Henck, Vivien; Wieloch, Tadeusz LU and Ruscher, Karsten LU (2017) In BMC Neuroscience 18(1).
Abstract

Background: The fractalkine/CX3C chemokine receptor 1 (CX3CR1) pathway has been identified to play an essential role in the chemotaxis of microglia, leukocyte trafficking and microglia/macrophage recruitment. It has also been shown to be important in the regulation of the inflammatory response in the early phase after experimental stroke. The present study was performed to investigate if CX3CR1 deficiency affects microglia during the first 14days with consequences for tissue damage after experimental stroke. Results: CX3CR1 deficiency significantly increased the number of intersections of GFP positive microglia in the proximal peri-infarct area at 2, 7 and 14days following tMCAO compared to heterozygous... (More)

Background: The fractalkine/CX3C chemokine receptor 1 (CX3CR1) pathway has been identified to play an essential role in the chemotaxis of microglia, leukocyte trafficking and microglia/macrophage recruitment. It has also been shown to be important in the regulation of the inflammatory response in the early phase after experimental stroke. The present study was performed to investigate if CX3CR1 deficiency affects microglia during the first 14days with consequences for tissue damage after experimental stroke. Results: CX3CR1 deficiency significantly increased the number of intersections of GFP positive microglia in the proximal peri-infarct area at 2, 7 and 14days following tMCAO compared to heterozygous and wildtype littermates. In addition, the length of microglial branches increased until day 7 in CX3CR1 knockout mice while the presence of a functional CX3CR1 allele resulted in a gradual reduction of their length following tMCAO. After stroke, wildtype, heterozygous and CX3CR1 deficient mice did not show differences in the composite neuroscore and assessment of infarct volumes from CX3CR1 wildtype, heterozygous and deficient mice revealed no differences between the genotypes 7 and 14days after stroke. Conclusion: Results demonstrate that CX3CR1 deficiency affects the morphology of GFP positive microglia located in the proximal peri-infarct region during the first 14days after tMCAO. Our data also indicate that CX3CR1 deficiency does not affect definite infarct volumes. Modulation of the CX3CR1 pathway may have implication for microglia function contributing to mechanisms of tissue reorganization in the post-ischemic brain.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
BMC Neuroscience
volume
18
issue
1
publisher
BioMed Central
external identifiers
  • scopus:85008395770
  • wos:000392144200001
ISSN
1471-2202
DOI
10.1186/s12868-016-0325-0
language
English
LU publication?
yes
id
ccb2ede2-da89-4cca-8ae4-d6ffe3f490c4
date added to LUP
2017-03-02 13:54:33
date last changed
2018-03-18 05:10:22
@article{ccb2ede2-da89-4cca-8ae4-d6ffe3f490c4,
  abstract     = {<p>Background: The fractalkine/CX<sub>3</sub>C chemokine receptor 1 (CX<sub>3</sub>CR1) pathway has been identified to play an essential role in the chemotaxis of microglia, leukocyte trafficking and microglia/macrophage recruitment. It has also been shown to be important in the regulation of the inflammatory response in the early phase after experimental stroke. The present study was performed to investigate if CX<sub>3</sub>CR1 deficiency affects microglia during the first 14days with consequences for tissue damage after experimental stroke. Results: CX3CR1 deficiency significantly increased the number of intersections of GFP positive microglia in the proximal peri-infarct area at 2, 7 and 14days following tMCAO compared to heterozygous and wildtype littermates. In addition, the length of microglial branches increased until day 7 in CX<sub>3</sub>CR1 knockout mice while the presence of a functional CX3CR1 allele resulted in a gradual reduction of their length following tMCAO. After stroke, wildtype, heterozygous and CX3CR1 deficient mice did not show differences in the composite neuroscore and assessment of infarct volumes from CX3CR1 wildtype, heterozygous and deficient mice revealed no differences between the genotypes 7 and 14days after stroke. Conclusion: Results demonstrate that CX3CR1 deficiency affects the morphology of GFP positive microglia located in the proximal peri-infarct region during the first 14days after tMCAO. Our data also indicate that CX3CR1 deficiency does not affect definite infarct volumes. Modulation of the CX3CR1 pathway may have implication for microglia function contributing to mechanisms of tissue reorganization in the post-ischemic brain.</p>},
  articleno    = {11},
  author       = {van der Maten, Gerlinde and Henck, Vivien and Wieloch, Tadeusz and Ruscher, Karsten},
  issn         = {1471-2202},
  language     = {eng},
  month        = {01},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {BMC Neuroscience},
  title        = {CX<sub>3</sub>C chemokine receptor 1 deficiency modulates microglia morphology but does not affect lesion size and short-term deficits after experimental stroke},
  url          = {http://dx.doi.org/10.1186/s12868-016-0325-0},
  volume       = {18},
  year         = {2017},
}